微流控芯片上胰岛素样生长因子-1（IGF-1）、碱性成纤维细胞生长因子（bFGF）对兔关节软骨细胞体外增殖的影响

发布时间：2018-05-01 17:00

本文选题：软骨细胞 + 组织工程　；参考：《大连医科大学》2010年博士论文

【摘要】： 目的：微流控芯片平台上探索三维培养环境下胰岛素样生长因子-1(Insulin-Like Growth Factor-1, IGF-1)、碱性成纤维细胞生长因子(basic Fibroblast Growth Factor, bFGF)对软骨细胞体外增殖的影响；通过验证微流控平台的有效性将微流控芯片技术引入软骨组织工程领域。方法：微流控芯片上,分别在IGF-1、bFGF以及二者组合浓度梯度下三维培养软骨细胞,两周后计算软骨细胞增殖率并与96孔板传统方法所获得的数据进行比较。结果：IGF-1在57.14ng/ml产生最大增殖效应(平均倍增2.38倍),bFGF在5.72ng/ml产生最大增殖效应(平均倍增3.85倍),85.71ng/ml的IGF-1与1.43ng/ml bFGF的组合使软骨细胞产生最大的增殖效应(平均倍增4.76倍)；芯片与传统96孔板所得增殖率不存在统计学显著性差异。结论：一定浓度组合的IGF-1和bFGF可协同产生最大的增殖效应；微流控芯片技术可有效应用于软骨组织工程领域,其在软骨组织工程领域存在广阔的应用前景。
[Abstract]:Objective: to explore the effects of insulin-like Growth Factor-1 (IGF-1) and basic fibroblast growth factor (basic Fibroblast Growth Factor, bFGF) on the proliferation of chondrocytes in three dimensional culture on microfluidic chip. The microfluidic chip technology is introduced into the cartilage tissue engineering field by verifying the effectiveness of the microfluidic platform. Methods: the chondrocytes were cultured on microfluidic chip under IGF-1bFGF and their combined concentration gradient. The proliferation rate of chondrocytes was calculated two weeks later and compared with the data obtained by the traditional method of 96-well plate. Results the maximum proliferative effect (average doubling 2.38 times) of 57.14ng/ml was produced by the combination of IGF-1 and 1.43ng/ml bFGF (the combination of IGF-1 and 1.43ng/ml bFGF was 3.85 times and 85.71ng / ml), and the proliferation effect of chondrocytes was 4.76 times (average doubling 4.76 times). There was no significant difference in the proliferation rate between the plate and the traditional 96 hole plate. Conclusion: the combination of IGF-1 and bFGF with a certain concentration can produce the greatest proliferative effect and microfluidic chip technology can be effectively applied in cartilage tissue engineering and has a broad application prospect in the field of cartilage tissue engineering.
【学位授予单位】：大连医科大学
【学位级别】：博士
【学位授予年份】：2010
【分类号】：R329

【共引文献】