不同剂量野百合碱诱导大鼠肺动脉高压模型的实验研究

发布时间：2018-05-04 23:10

本文选题：肺动脉高压 + 野百合碱　；参考：《中西医结合心脑血管病杂志》2015年10期

【摘要】：目的观察不同剂量的野百合碱诱导大鼠肺动脉高压模型的建立,探索合适的野百合碱造模剂量。方法将60只雄性SD大鼠随机分成4组,正常对照组、野百合碱低剂量组、野百合碱中剂量组和野百合碱高剂量组,一次性给予颈背部皮下注射不同剂量野百合碱诱导建立肺动脉高压大鼠模型。4周后,测量各组大鼠肺动脉压力、心输出量、肺血管阻力以及右心室肥厚程度。结果野百合碱高剂量组大鼠死亡率、肺动脉压力、右心室室壁厚度高于中剂量组、低剂量组和正常对照组(P0.001),心输出量低于中剂量组、低剂量组和正常对照组(P0.001);高剂量组肺动脉压力、肺血管阻力高于中剂量组、低剂量组(P0.001)。结论中剂量组野百合碱诱导的大鼠肺动脉高压模型更为接近大鼠肺动脉模型的理想数值。
[Abstract]:Objective to observe the establishment of pulmonary hypertension model induced by monocrotaline in rats and to explore the appropriate dose of monocrotaline. Methods Sixty male Sprague-Dawley rats were randomly divided into 4 groups: normal control group, low dose monocrotaline group, middle dose monocrotaline group and high monocrotaline group. Pulmonary artery pressure, cardiac output, pulmonary vascular resistance and right ventricular hypertrophy were measured in rats with pulmonary hypertension induced by subcutaneous injection of monocrotaline. Results the mortality, pulmonary artery pressure and right ventricular wall thickness in the high dose group of monocrotaline were higher than those in the middle dose group, while in the low dose group and the normal control group, the cardiac output was lower than that in the middle dose group. The pulmonary artery pressure and pulmonary vascular resistance in the high dose group were higher than those in the middle dose group. Conclusion the model of pulmonary hypertension induced by monocrotaline in middle dose group is closer to the ideal value of pulmonary artery model in rats.
【作者单位】：河南中医学院第一附属医院;
【基金】：国家自然科学基金资助项目(No.81273948)
【分类号】：R544.1;R-332

【参考文献】