碱性成纤维细胞生长因子调节大鼠心脏成纤维细胞β-catenin的表达

发布时间：2018-05-07 13:44

本文选题：低氧/复氧损伤 + 碱性成纤维细胞生长因子　；参考：《兰州大学》2010年硕士论文

【摘要】： 目的：探讨碱性成纤维细胞生长因子(bFGF)对心脏成纤维细胞低氧／复氧损伤后Wnt通路中β-catenin蛋白表达的调节作用及其机制。方法：采用物理缺氧法制作体外培养SD大鼠心脏成纤维细胞低氧／复氧损伤模型。将细胞随机分6组(1)对照组：细胞置于常氧培养箱内持续孵育。(2)低氧／复氧组(损伤组)：细胞置于低氧培养箱中低氧培养24小时,然后置于常氧培养箱中复氧培养半小时。(3-6)碱性成纤维细胞生长因子+低氧／复氧组(碱性成纤维细胞生长因子干预组),细胞低氧／复氧后以碱性成纤维细胞生长因子(basic fibroblast growth factor, bFGF)处理(碱性成纤维细胞生长因子浓度5μg/L、10μg/L、20μg/L、40μg/L)。实验采用采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)比色法、末端脱氧核糖核酸转移酶介导的dUTP切口末端标记(TUNEL)法、免疫荧光法分析检测心脏成纤维细胞相对活力、凋亡百分率、P-catenin蛋白的平均荧光值。结果：SD (Sprague Dawley)大鼠低氧／复氧后MTT检测结果提示碱性成纤维细胞生长因子+低氧／复氧组较低氧／复氧损伤组增值率增加(P0.05),而激光共聚焦显微镜结果提示碱性成纤维细胞生长因子+低氧／复氧组β-catenin蛋白平均荧光值高于低氧／复氧损伤组(P0.05),β-catenin蛋白增多；TUNEL阳性反应产物百分比较低氧／复氧损伤组减少(P0.05)。结论：碱性成纤维细胞生长因子能够减少低氧／复氧损后心脏成纤维细胞凋亡,并参与了Wnt通路中β-catenin蛋白表达的调节,对低氧／复氧损伤后的心脏成纤维细胞具有促进增殖作用。
[Abstract]:Aim: to investigate the effect of basic fibroblast growth factor (bFGF) on the expression of 尾 -catenin protein in Wnt pathway of cardiac fibroblasts after hypoxia / reoxygenation injury. Methods: hypoxia / reoxygenation injury model of rat cardiac fibroblasts in vitro was established by physical hypoxia. The cells were randomly divided into 6 groups: control group: the cells were incubated in a normoxic incubator for 24 hours in hypoxia / reoxygenation group (injury group: cells were incubated in hypoxia incubator for 24 hours). Then incubated in a normoxic incubator for half an hour after reoxygenation, the basic fibroblast growth factor hypoxia / reoxygenation group (basic fibroblast growth factor intervention group), cells hypoxia / reoxygenation after the basic fibroblast growth factor growth Basic fibroblast growth factor (bFGF) treatment (concentration of basic fibroblast growth factor 5 渭 g / L ~ 10 渭 g / L ~ (10) 渭 g / L ~ (20) 渭 g / L ~ (20) 渭 g / L ~ (40) 渭 g / L ~ (-1)). The relative viability of cardiac fibroblasts was detected by the method of 3- (4-dimethylthiazole-2-dimethylthiazolium) -2-diphenyltetrazolium bromide (MTT) colorimetry, terminal deoxyribonucleic acid transferase-mediated dUTP Nick end labeling (Tunel) method, and immunofluorescence assay. Percentage of apoptosis and average fluorescence value of P-catenin protein. Results the results of MTT detection after hypoxia / reoxygenation showed that the proliferation rate of basic fibroblast growth factor hypoxia / reoxygenation group was higher than that of hypoxia / reoxygenation injury group, while the results of laser confocal microscopy showed that basic fibroblast growth factor (bFGF) fibroblast growth factor in hypoxia / reoxygenation group was higher than that in hypoxia / reoxygenation group. The average fluorescence value of 尾 -catenin protein in the hypoxia / reoxygenation group was higher than that in the hypoxia / reoxygenation group, and the percentage of Tunel positive reaction products in the hypoxia / reoxygenation group was lower than that in the hypoxia / reoxygenation group. Conclusion: basic fibroblast growth factor can reduce the apoptosis of cardiac fibroblasts after hypoxia / reoxygenation, and participate in the regulation of 尾 -catenin protein expression in Wnt pathway. It can promote the proliferation of cardiac fibroblasts after hypoxia / reoxygenation injury.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R329

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