人胚胎多潜能干细胞向心肌样细胞转化的研究

发布时间：2018-05-13 00:33

本文选题：5-氮胞苷 + 多潜能干细胞　；参考：《吉林大学》2009年硕士论文

【摘要】： 急性心肌梗死(acute myocardial infarction,AMI)是严重的心血管系统疾病,威胁着人类的健康。目前包括药物治疗、介入治疗及手术治疗等各种临床治疗手段,虽然在一定程度上可改善患者的症状,但均不能再生坏死的心肌。因此,干细胞移植成为心肌损伤治疗一条很好的途径。本实验以人胚胎肝组织来源多潜能干细胞为研究对象,以5-aza为诱导剂,在体外将其诱导分化为心肌样细胞,通过形态学,RT-PCR,免疫细胞化学等方法,对诱导后细胞进行鉴定,探讨人胚胎肝组织来源多潜能干细胞分化为心肌样细胞的潜能及其分化机制。结果显示,体外人胚胎肝组织来源多潜能干细胞在适当的5-aza作用下,可分化为心肌样细胞,RT-PCR检测到人心肌细胞基因的表达。为今后治疗心脏病药物的研发及干细胞移植治疗提供了一定的理论依据和实验基础。
[Abstract]:Acute Myocardial Infarction (AMI) is a serious cardiovascular disease that threatens human health. At present, all kinds of clinical treatments, such as drug therapy, interventional therapy and surgical treatment, can improve the symptoms of patients to some extent, but can not regenerate necrotic myocardium. Therefore, stem cell transplantation is a good way to treat myocardial injury. In this study, multipotential stem cells derived from human embryonic liver tissue were used as the research object, 5-aza was used as inducer, the cells were induced to differentiate into cardiomyocyte-like cells in vitro, and the induced cells were identified by morphology RT-PCR, immunocytochemistry and so on. To investigate the potential and mechanism of multipotential stem cells derived from human embryonic liver tissue to differentiate into cardiomyocyte-like cells. The results showed that the multipotential stem cells derived from human embryonic liver tissue could differentiate into cardiomyocyte like cells by RT-PCR and detect the expression of human cardiomyocyte gene by RT-PCR. It provides a theoretical and experimental basis for the future development of heart disease drugs and stem cell transplantation therapy.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R329

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