SD大鼠慢性非细菌性前列腺炎模型的建立及其生物学特性研究

发布时间：2018-05-17 16:56

本文选题：SD大鼠 + 模型　；参考：《复旦大学》2008年硕士论文

【摘要】： 目的: 建立两种SD大鼠慢性非细菌性前列腺炎的模型,研究其生物学特性,探讨慢性非细菌性前列腺炎的发病机制,观察模型对生殖功能的影响,观察舍尼通对模型的疗效,探讨舍尼通的作用机理。方法: 1、模型一:自体免疫性慢性非细菌性前列腺炎模型的建立(简称免疫性模型) 32只大鼠随机分成4组,其中一组为阴性对照组,其它三组为试验组,第0、7天多点皮内注射浓度分别为20、40、80mg/ml的大鼠前列腺蛋白提纯液联合完全弗氏佐剂(1:1的混悬液)1.0ml,腹腔注射百白破疫苗0.5ml,第21天腹腔注射大鼠前列腺蛋白提纯液0.5ml,阴性对照组在相同时间和部位注射等量的生理盐水,造模后第35天处死大鼠。 2、模型二:化学性慢性非细菌性前列腺炎模型的建立(简称化学性模型) 16只大鼠随机分为两组,阴性对照组和模型组。模型组用1%λ-角叉菜胶生理盐水溶液0.1ml直接注射前列腺内,阴性对照组注射生理盐水。造模后第35天处死大鼠。 3、观察大鼠的前列腺大体和组织病理学变化,评价模型是否成功,并测定血清中的Ca~(2+)、Mg~(2+)、雌二醇(E_2)、睾酮(T)、双氢睾酮(DHT)含量,观察其生物学特性变化,初步探讨慢性非细菌性前列腺炎的发病机制。 4、造模成功后,雌雄鼠1:1交配,观察雌鼠的妊娠率,仔鼠的生长发育,评价模型对生殖功能的影响。 5、用阳性药舍尼通模拟临床途径灌胃给药,观察舍尼通对两种模型的疗效,初步探讨舍尼通的作用机理。结果: 1、在大鼠前列腺蛋白提纯液联合双重免疫佐剂制作的SD大鼠自体免疫性前列腺炎模型中,高剂量(80mg/ml)模型组的前列腺部分腺腔破坏,明显的充血、水肿,间质和腺腔有淋巴细胞和单核细胞浸润,呈现明显的慢性炎症表现。 2、在SD大鼠前列腺内注射1%λ-角叉菜胶生理盐水溶液制作的化学性前列腺炎模型中,观察到前列腺间质有大量淋巴细胞和单核细胞侵润,血管增生和充血明显,呈现明显的慢性炎症表现。 3、在免疫模型组中,与阴性对照组相比,高剂量模型组的血液白细胞数明显升高(P≤0.05):低、中剂量模型组的血清Ca~(2+)升高,但差别不显著(P＞0.05),高剂量模型组的血清Ca~(2+)明显降低(P≤0.05);低、中、高剂量模型组的血清IgG均明显升高(P≤0.01);低剂量模型组的血清T明显升高(P≤0.05),中、高剂量模型组的血清T也升高,但差别不显著(P＞0.05);中、高剂量模型组的DHT升高,且高剂量模型组升高的幅度较大,但差别不显著(P＞0.05)。 4、在1%角叉菜胶模型组中,与阴性对照组相比,模型组的血清双氢睾酮含量明显降低(P≤0.01);前列腺脏器系数明显下降,说明前列腺重量明显减轻(P≤0.05);血清中的T、Ca~(2+)和Mg~(2+)浓度升高,但差别不显著(P＞0.05)。 5、两种模型对生殖的影响:精子活力分析结果为:A、B、D级活力的精子:阴性对照组与1%角叉菜胶模型组之间有显著差异(P≤0.05),C级活力的精子:阴性对照组与1%角叉菜胶模型组之间无显著差异(P≥0.05),各级活力的精子在阴性对照组和免疫模型组之间均无显著差异(P≥0.05);雌鼠妊娠率、产仔数和仔鼠出生的性别比例及外观畸形数和死亡数:三组之间无明显差别;仔鼠的前列腺病理学表现:与阴性对照组相比,模型组的仔鼠前列腺无明显改变,都是光滑、无结节,无充血水肿,无炎细胞侵润。 6、舍尼通对两种模型的作用:对免疫模型的作用表现在显著升高血清中的Ca~(2+)水平和T水平(P≤0.05);对化学模型的作用表现在用药后血液中的白细胞数显著下降(P≤0.05):对前列腺的组织病理学改变都显示出充血水肿减轻。结论: 1、第0、7天多点皮内注射浓度为80mg/ml的大鼠前列腺蛋白提纯液联合完全弗氏佐剂(1:1的混悬液),腹腔注射百白破疫苗0.5ml,第21天腹腔注射大鼠前列腺蛋白提纯液0.5ml,造模35天可以成功制作SD大鼠自体免疫性慢性前列腺炎的模型。 2、采用1%λ-角叉菜胶生理盐水溶液0.1ml直接注射前列腺内,造模35天可以成功制作SD大鼠化学性慢性非细菌性前列腺炎的模型。 3、化学性模型能明显降低精子活力,两种模型对雌鼠妊娠率、仔鼠的生长发育无明显影响。 4、舍尼通对两种慢性前列腺炎均有效,但作用机制不同。对免疫模型是通过升高Ca~(2+)和T的水平,对化学性模型是通过减少白细胞数来起作用。
[Abstract]:Objective:
To establish a model of two chronic non bacterial prostatitis in SD rats, study its biological characteristics, explore the pathogenesis of chronic non bacterial prostatitis, observe the effect of the model on the reproductive function, observe the effect of the model and explore the mechanism of the action of the model.
Method:
1, model 1: establishment of autoimmune chronic nonbacterial prostatitis model (Immune Model).
32 rats were randomly divided into 4 groups. One group was a negative control group and the other three groups were in the experimental group. The rat prostatic protein purify solution (20,40,80mg/ml) combined with complete Freund's adjuvant (1:1 suspension) 1.0ml, intraperitoneal injection of pestilence 0.5ml in the abdominal cavity and intraperitoneal injection of prostatic protein purification in the abdominal cavity on day twenty-first. The rats in the negative control group were injected with the same amount of normal saline at the same time and place, and rats were killed thirty-fifth days after modeling. 0.5ml
2, model two: establishment of a chemical chronic non bacterial prostatitis model (chemical model).
16 rats were randomly divided into two groups, the negative control group and the model group. The model group was injected with 1% lambda carrageenan physiological saline solution 0.1ml directly into the prostate, and the negative control group was injected with saline. The rats were killed thirty-fifth days after building the model.
3, observe the general and histopathological changes of the prostate in rats, evaluate the success of the model, and determine the content of Ca~ (2+), Mg~ (2+), estradiol (E_2), testosterone (T), and dihydrotestosterone (DHT) in the serum, and observe the changes in the biological characteristics of the rats. The pathogenesis of chronic non bacterial prostatitis is preliminarily discussed.
4, after successful modeling, male and female mice 1:1 mating, observe the pregnancy rate of female rats, the growth and development of offspring rats, and evaluate the effect of the model on reproductive function.
5, we used the positive drug Shinto Tong to simulate the clinical route of gavage, observe the curative effect of shinton on the two models, and preliminarily explore the mechanism of the effect of shinby.
Result:
1, in the SD rat autoimmune prostatitis model of rat prostatic protein purification fluid combined with double immuno adjuvant, the partial adenocarcinoma of the prostate in the high dose (80mg/ml) model group was obviously congested, edema, interstitial and adenocavities were infiltrated with lymphocytes and mononuclear cells, showing obvious chronic inflammatory performance.
2, in the chemical prostatitis model produced by injection of 1% lambda - carrageenan saline solution in the prostate of SD rats, a large number of lymphocytes and monocytes were infiltrated, vascular proliferation and hyperemia were evident in the prostatic interstitium, showing obvious chronic inflammatory manifestations.
3, in the immune model group, the number of white blood cells in the high dose model group was significantly higher than that in the negative control group (P < 0.05). The serum Ca~ (2+) in the middle dose model group increased, but the difference was not significant (P > 0.05). The serum Ca~ (2+) of the high dose model group decreased significantly (P < 0.05), and the serum IgG in the high dose model group increased significantly (P). In the low dose model group, the serum T increased significantly (P < 0.05). In the high dose model group, the serum T increased, but the difference was not significant (P > 0.05). In the high dose model group, the DHT increased, and the high dose model group increased significantly, but the difference was not significant (P > 0.05).
4, in the 1% corner carrageenan model group, the serum dihydrotestosterone content of the model group was significantly lower than that of the negative control group (P < 0.01), and the prostate organ coefficient decreased significantly, indicating that the prostate weight was significantly reduced (P < 0.05), and the concentration of T, Ca~ (2+) and Mg~ (2+) in the serum was increased, but the difference was not significant (P > 0.05).
5, the effect of two models on reproduction: sperm motility analysis results were: A, B, D class vitality sperm: negative control group and 1% carrageenan model group significant difference (P < 0.05), C class vitality sperm: negative control group and 1% corner carrageenan model group no significant difference (P > 0.05), the vitality of sperm at all levels in negative control and immunization There was no significant difference between the two groups (P > 0.05). The pregnancy rate, the number of offspring and the sex ratio of the offspring, the number of deformities and the number of deaths were no significant difference between the three groups. No inflammatory cells invade.
6, the effect of seronont on the two models: the effect on the immune model was shown to significantly increase the level of Ca~ (2+) and T (P < 0.05) in the serum; the effect of the chemical model on the number of white blood cells in the blood decreased significantly (P < 0.05) after the drug use: the histopathological changes in the prostatic group showed the reduction of congestion and edema.
Conclusion:
1, on day 0,7, the rat prostatic protein purify solution with intradermal injection of 80mg/ml combined with complete Freund's adjuvant (1:1 suspension), intraperitoneal injection of pertussis vaccine 0.5ml, and intraperitoneal injection of prostatic protein purified liquid 0.5ml in rats on the twenty-first day, and the model of autoimmune chronic prostatitis in SD rats could be successfully made for 35 days.
2, 1% lambda carrageenan physiological saline solution 0.1ml was injected into the prostate directly. The model of chemical chronic non bacterial prostatitis in SD rats could be successfully made for 35 days.
3, the chemical model can significantly reduce sperm motility, and the two models have no significant effect on the pregnancy rate and the growth and development of offspring rats.
4, all of the two kinds of chronic prostatitis were effective, but the mechanism was different. The immune model was used to increase the level of Ca~ (2+) and T, and the chemical model was used to reduce the number of white cells.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R-332;R697.33

【引证文献】