龟芪生精胶囊对家兔生精障碍模型促生精作用的实验研究

发布时间：2018-05-20 22:43

本文选题：龟芪生精胶囊 + 生精障碍　；参考：《昆明医科大学》2013年硕士论文

【摘要】：[目的]建立家兔机械性生精障碍模型,通过分析家兔精液参数变化和睾丸组织学改变,观察龟芪生精胶囊对机械性生精障碍模型家兔的促生精作用,为中药治疗男性不育提供实验依据。 [方法]以手术方法复制家兔单侧(日本雄性大耳白兔)、双侧(雄性加利福尼亚兔)睾丸生精障碍模型,并分别根据是否进行睾丸下降固定术予以分为不同的亚组,以黄精赞育胶囊和温水为对照,通过纵向和横向比较,观察龟芪生精胶囊对各组家兔的精子密度、活力、活率和精液量的影响及睾丸组织病理变化的影响。 [结果]1.日本雄性大耳白兔单侧生精障碍模型组： 1.1单侧造模前后、行睾丸下降固定或不予下降处理的各组纵向比较精子密度、活力、活率均具有显著差异(均为P0.05),精液量无显著差异(均为P0.05),睾丸组织病理改变明显。 1.2单侧造模后行睾丸下降固定手术后4个月的各亚组(a1、a2、a3、c1、 c2、c3)与未行睾丸下降处理4个月的各亚组(b1、b2、b3、d1、d2、d3)相比较(即a1与b1、a2与b2、a3与b3、c1与d1、c2与d2、c3与d3比较)精子密度、活力、活率具有显著差异(均为P0.05),精液量无显著差异(均为P0.05),睾丸组织病理变化明显。 1.3单侧造模后行睾丸下降固定手术后4个月的各亚组a1、a2、a3之间及c1、c2、c3之间两两比较精子密度、活力、活率及精液量,a2与a1、c2与c1有显著差异(P0.05),a3与al、c3与c1有显著差异(P0.05),a3与a2、c3与c2无明显差异(P0.05),各组间精液量无明显差异(均为P0.05)。单侧造模后未行睾丸下降处理4个月后的各亚组b1、b2、b3之间及d1、d2、d3之间两两比较精子密度、活力、活率及精液量都无明显差异(均为P0.05)。 2.雄性加利福尼亚兔双侧生精障碍组： 2.1双侧造模前后、行睾丸下降固定或不予下降处理的各组纵向比较精子密度、活力、活率均具有显著差异(均为P0.05),精液量无显著差异(均为P0.05),睾丸组织病理改变明显。 2.2双侧造模后行睾丸下降固定手术后4个月的各亚组(B1、B2、B3、C1、 C2、C3、D1、D2、D3)与未行睾丸下降处理4个月的各亚组(A1、A2、A3)相比较(即B1与A1、B2与A2、B3与A3、C1与A1、C2与A2、C3与A3、D1与A1、D2与A2、D3与A3比较)精子密度、活力、活率具有显著差异(均为P0.05),精液量无显著差异(均为P0.05)；双侧造模后行单侧睾丸下降固定各亚组(B1、 B2、B3、C1、C2、C3)与行双侧睾丸下降固定各亚组(D1、D2、D3)相比较(即B1与Dl、B2与D2、B3与D3、C1与D1、C2与D2、C3与D3比较)精子密度、活力、活率具有显著差异(均为P0.05),精液量无显著差异(均为P0.05)。 2.3双侧造模后行睾丸下降固定手术后4个月的各亚组B1、B2、B3之间、C1、C2、C3之间及D1、D2、D3之间两两比较精子密度、活力、活率及精液量,B2与B1、C2与C1、D2与D1有显著差异(P0.05),B3与B1、C3与C1、D3与D1有显著差异(P0.05),B3与B2、C3与C2、D3与D2无明显差异(P0.05),各组间精液量无明显差异(均为P0.05)。双侧造模后未行睾丸下降处理4个月后的各亚组A1、A2、A3之间两两比较精子密度、活力、活率及精液量都无明显差异(均为P0.05)。 [结论] 1.温度因素对家兔睾丸生精障碍的影响具有一定的可逆性。 2.造成生精障碍的诱因(温度)不解除,家兔精液质量(精子密度、活力、活率)呈进行性下降,睾丸组织病理变化继续加重。 3.解除造成生精障碍的诱因(温度)后再给予生精药物治疗能更好的促进家兔精液质量(精子密度、活率、活力)及睾丸组织病理变化的恢复,且龟芪生精胶囊与已经上市的国药准字号产品黄精赞育胶囊都有较好的疗效。这为以后研究龟芪生精胶囊的作用机理以及进行临床试验奠定了实验基础。
[Abstract]:[Objective] to establish a model of mechanical spermatogenesis disorder in rabbits. By analyzing the changes of the semen parameters and the histological changes of the testis in rabbits, the effect of turtling Qi Shengjing Capsule on the promoting spermatogenic effect of the rabbit model of mechanical spermatogenesis disorder was observed, and the experimental basis for the treatment of male infertility was provided.
[Methods] the unilateral (male, Japanese white rabbit) and bilateral (male California rabbit) model of testicular spermatogenesis were replicated by the operation method, and the testicular descent fixation was divided into different subgroups according to whether the testicular descent fixation was carried out. The effects of rabbit sperm density, viability, viability and semen volume on the pathological changes of testicular tissue were observed.
[results]1. model of unilateral spermatogenesis disorder in Japanese male white rabbits was established.
1.1 before and after the unilateral model, the sperm density, vitality and survival rate of each group with declines or no treatment were significantly different (all P0.05), and the amount of semen was not significantly different (all P0.05), and the pathological changes of the testicular tissue were obvious.
1.2 the subgroups (A1, A2, A3, C1, C2, C3) were compared with those of the subgroups (B1, B2, B3, D1, D2, D3) for 4 months after the 4 months after the unilateral model of the testicular descending operation (B1, B2, B3, D1, D2, D3). The pathological changes of the testis were obvious (all P0.05).
1.3 A1, A2, A3 and C1, C2, C3 between the subgroups of the unilateral model after the descending operation of the testis, and C1, C2, and C3, compared with the sperm density, vigor, the viability and the semen quantity, A2 and A1, C2 and C1 significant difference (P0.05). All of the subgroups of B1, B2, B3 and D1, D2, D3 after 4 months after the unilateral model of the unilateral model were compared with D1, D2, and D3, and there were no significant differences in sperm density, activity, viability and semen quantity (P0.05).
2. male California rabbit bilateral spermatogenic disorder group:
2.1 there were significant differences in sperm density, vitality and survival rate (all P0.05), and no significant difference in semen volume (all P0.05), and the pathological changes of testicular tissue were obvious in each group.
2.2 compared with each subgroup (B1, B2, B3, C1, C2, C3, D1, D2, D3) in the 4 months after the bilateral testicular descending operation (D1, D2, D3) in each subgroup (A1, A2, A3) There was no significant difference in semen volume (all P0.05), and bilateral testicular descending groups (B1, B2, B3, C1, C2, C3) were compared with each subgroup (D1, D2, D3) of bilateral testicular descent (D1, D2, D3) in bilateral spermatozoa after bilateral model building. Significant differences (all P0.05).
2.3 B1, B2, B3, C1, C2, C3, and D1, D2, and D3 were compared with D1, D2, and D3, compared with D1, D2, and D3, and there were significant differences between B2 and B1 (B2 and B1). 5) there was no significant difference between the semen of each group (all P0.05). There was no significant difference in sperm density, activity, viability and semen amount (all P0.05) between the subgroups of A1, A2, and A3 after 4 months after bilateral modeling.
[Conclusion]
1. the influence of temperature on spermatogenesis impairment of rabbit testis is reversible.
2. the semen quality (sperm density, vitality, viability) decreased progressively, and the pathological changes of testicular tissue continued to increase.
3. relieving the cause of spermatogenesis disorder (temperature) and giving the treatment of spermatogenic drugs can better promote the quality of the rabbit's semen (sperm density, survival rate, vitality) and the recovery of the pathological changes of the testis. The mechanism of Shengjing capsule and its clinical trials have laid an experimental foundation.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R277.5;R-332

【参考文献】