舒郁胶囊对PMS肝气郁证模型大鼠下丘脑μ-阿片受体的影响

发布时间：2018-05-21 02:32

本文选题：舒郁胶囊 + PMS肝气郁证　；参考：《中国实验方剂学杂志》2015年13期

【摘要】：目的:通过观察经前期综合征(PMS)肝气郁证模型大鼠下丘脑中μ-阿片受体(MOR)的蛋白表达,探讨舒郁胶囊治疗PMS肝气郁证的机制。方法:Wistar大鼠56只,随机分为正常组、模型组、舒郁胶囊组(0.408 g·kg-1)和纳洛酮组(0.4mg·kg-1),每组14只,旷场实验和阴道涂片筛选动情周期规律非接受期大鼠进入实验,除正常组,其余各组采用慢性束缚应激法复制PMS肝气郁证模型,旷场实验对模型进行行为学评价,各组大鼠下丘脑采用免疫荧光化学技术(IF)和Western blot分别观察MOR分布及蛋白表达变化。结果:宏观行为学表明,与正常组比较,模型组大鼠旷场总分显著降低(P0.01);免疫荧光结果显示,与正常组比较,模型组大鼠下丘脑细胞分布排列杂乱且增多,与模型组比较,舒郁胶囊、纳洛酮给药组均得到显著改善;蛋白免疫印迹结果显示,与正常组比较,模型组下丘脑中MOR表达水平显著升高(P0.01),与模型组比较,舒郁胶囊、纳洛酮给药组MOR表达水平均显著下降(P0.01)。结论:舒郁胶囊可通过对下丘脑中MOR的拮抗作用而改善PMS肝气郁证的症状,另外,下丘脑中MOR上调可能是PMS肝气郁证的发病机制之一。
[Abstract]:Objective: to observe the protein expression of 渭 -opioid receptor moor in hypothalamus of rats with PMS-PMS-liver-qi depression syndrome, and to explore the mechanism of Shuyu capsule in treating PMS syndrome of liver-qi depression. Methods Fifty-six Wistar rats were randomly divided into normal group, model group, Shuyu capsule group (0.408 g 路kg -1) and naloxone group (14 rats in each group). Open field test and vaginal smear were used to screen non-accepted rats in estrus cycle, except the normal group. The other groups were used to establish the model of PMS liver qi stagnation by chronic restraint stress. The behavior of the model was evaluated by open field experiment. The distribution of MOR and the expression of protein were observed by immunofluorescence technique and Western blot in hypothalamus of rats in each group. Results: compared with the normal group, the total open field score of the model group was significantly lower than that of the normal group, and the immunofluorescence results showed that the distribution of hypothalamus cells in the model group was disordered and increased compared with the normal group, and compared with the model group, the results of immunofluorescence showed that the distribution of hypothalamus cells in the model group was disordered and increased compared with the model group. The results of Western blot showed that the expression of MOR in the hypothalamus of the model group was significantly higher than that in the normal group, and that in the model group, compared with the model group, the expression of MOR in the hypothalamus of the model group was significantly higher than that in the model group, and the expression of MOR in the hypothalamus of the model group was significantly higher than that in the model group. The expression of MOR in naloxone group decreased significantly (P 0.01). Conclusion: Shuyu capsule can improve the symptoms of liver qi depression syndrome of PMS by antagonistic effect of MOR in hypothalamus. In addition, upregulation of MOR in hypothalamus may be one of the pathogenesis of PMS liver qi depression syndrome.
【作者单位】：山东中医药大学中医药经典理论教育部重点实验室;威海市立医院;
【基金】：国家自然科学基金面上项目(81173162)
【分类号】：R285.5;R-332

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