双足大鼠脊柱侧弯模型的建立

发布时间：2018-07-08 08:18

本文选题：脊柱侧弯 + 双足大鼠　；参考：《复旦大学》2009年硕士论文

【摘要】： 目的青少年特发性脊柱侧弯(adolescent idiopathic scoliosis,AIS)是一种严重危害青少年健康的疾病,AIS已经是青少年继视力异常、肥胖、包茎和社会心理障碍后的第五大常见病。它的病因至今未明,其中病因研究的热点集中到神经内分泌系统,而褪黑素与钙调蛋白的研究更是炙手可热,已有研究表明褪黑素减少是其中一个重要病因,钙调蛋白与脊柱侧弯的严重性成正比。动物模型是病因学研究的基础。很多研究通过已知的病因制作了许多动物模型,但是至今仍未找到制作理想脊柱侧弯动物模型的办法。本实验希望通过向双足大鼠腹腔注射褪黑素拮抗剂制作特发性脊柱侧弯模型,从而探讨褪黑素、钙调蛋白与特发性脊柱侧弯的关系,为进一步研究其病因奠定一定的实验基础。方法健康的刚断乳的SD大鼠30只,适应性生长一周,随机分为5组,每组为6只。ABCD四组为双足大鼠组,截去双前肢和尾巴。ABC三组每天晚上6点到8点,腹腔注射褪黑素拮抗剂luzindole,剂量分别为0.2mg/kg、0.5mg/kg、1mg/kg,使用生理盐水的体积均为10ml。D组每天相同时间腹腔注射相同体积的生理盐水。E组为四足大鼠组,作为空白对照。处死前,抽各组的静脉血,离心分离出血小板,用ELISA法测定各组血小板中的钙调蛋白水平。结果 1.ABC三组均发生了脊柱侧弯,发生率为38.9%,其中A组发生的脊柱侧弯显著,DE两组均未发生脊柱侧弯,未发生率为100%。 2.五组平均体重最重的是注射褪黑素拮抗剂剂量最大的C组。 3.各组血小板中的钙调蛋白水平未见明显的差异(p＞0.05)。结论 1.本实验可成功建立双足大鼠脊柱侧弯模型,重复性好,并证实了脊柱侧弯的发生与褪黑素的减少有关。使用褪黑素拮抗剂luzindole的剂量0.2mg/kg能成功建立脊柱侧弯模型。 2.褪黑素拮抗剂luzindole可促进大鼠的生长。 3.血小板中的钙调蛋白水平与特发性脊柱侧弯未见明显的相关性。
[Abstract]:Objective: adolescent idiopathic scoliosis (adolescent idiopathic) is the fifth most common disease in adolescents after abnormal vision, obesity, phimosis and psychosocial disorders. The etiology of melatonin is still unknown, among which the focus of etiological research is focused on the neuroendocrine system, and the studies of melatonin and calmodulin are even hotter. It has been shown that the decrease of melatonin is one of the important causes. Calmodulin is proportional to the severity of scoliosis. Animal models are the basis of etiological research. Many studies have produced many animal models based on known etiology, but no method has been found to make ideal animal models of scoliosis. The purpose of this study was to investigate the relationship between melatonin, calmodulin and idiopathic scoliosis by intraperitoneal injection of melatonin antagonist in bipedal rats, so as to lay a foundation for further study on the etiology of idiopathic scoliosis. Methods Thirty healthy weaned SD rats were randomly divided into 5 groups: 6 rats in each group. ABCD group 4 was biped rats. The forelimbs and tail were amputated from 6 pm to 8 o'clock every night in the three groups. The dose of melatonin antagonist luzindolewas 0.2 mg / kg ~ 0.5 mg / kg ~ (-1 mg / kg), and the volume of normal saline was 10 ml 路d ~ (-1) mg 路kg ~ (-1) 路g ~ (-1) 路kg ~ (-1) respectively. Before death, platelets were isolated by centrifugation, and calmodulin levels in platelets were measured by Elisa. Results 1. The incidence of scoliosis was 38.9 in ABC group, and there was no scoliosis in group A (100.2%). The heaviest average weight of the five groups was group C, which received the largest dose of melatonin antagonist. There was no significant difference in calmodulin levels in platelets of each group (p > 0.05). Conclusion 1. The results showed that the occurrence of scoliosis was related to the decrease of melatonin. The model of scoliosis was successfully established by using the dose 0.2mg/kg of melatonin antagonist luzindole. 2. Melatonin antagonist luzindole can promote the growth of rats. There was no significant correlation between calmodulin levels in platelets and idiopathic scoliosis.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R682;R-332

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