幽门螺杆菌对GES-1细胞BaxmRNA影响的研究

发布时间：2018-07-11 18:18

本文选题：幽门螺杆菌 + Bax　；参考：《现代诊断与治疗》2015年22期

【摘要】：目的通过不同基因型的幽门螺杆菌(Hp)培养上清作用GES-1细胞,研究细胞Bax表达的变化。方法分组:(1)不同浓度NCTC11637Hp上清液的试验组,浓度分别为A组(1.3875mg/ml),B组(2.775mg/ml),C组(5.55mg/ml);(2)不同浓度NCTC11639Hp上清液的试验组,浓度分别为A(1.875mg/ml),B(3.75mg/ml),C(7.5mg/ml);(3)阴性对照组(Hp培养液);(4)空白对照组(DMEM)。各组作用GES-1细胞时间均为24h、48h、72h,用RT-PCR检测mRNA的表达。结果 2.775mg/ml NCTC11637上清和3.75mg/ml NCTC11639上清作用GES-1细胞48h后BaxmRNA均显著上调(P0.01~0.05)。结论不同基因型的Hp培养上清液可上调胃黏膜上皮细胞内Bax的表达,提示Hp可能通过激活Bax而诱导细胞凋亡。
[Abstract]:Objective to study the changes of Bax expression in GES-1 cells cultured with different genotypes of Helicobacter pylori (HP). Methods: (1) the supernatants of NCTC11637Hp were divided into two groups: group A (1.3875mg/ml), group B (2.775mg/ml), group C (5.55mg/ml); (2), group A (1.875mg/ml), group B (3.75mg/ml), group C (7.5mg/ml); (3), blank control group (); (4). The time of GES-1 cells in each group was 24 h, 48 h and 72 h, and the mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results 2.775mg/ml NCTC11637 supernatant and 3.75mg/ml NCTC11639 supernatant significantly upregulated the expression of Bax mRNA in GES-1 cells for 48 h. Conclusion the supernatant of HP culture with different genotypes can up-regulate the expression of Bax in gastric mucosal epithelial cells, suggesting that HP may induce apoptosis by activating Bax.
【作者单位】：赣南医学院第一附属医院消化内科;南昌大学第一附属医院消化内科;
【基金】：国家自然科学基金资助课题;NO.30660067
【分类号】：R378

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