自旋捕捉剂修饰及生物学效应研究

发布时间：2018-07-11 22:01

本文选题：自旋捕捉剂 + 氧化还原状态　；参考：《浙江大学》2010年硕士论文

【摘要】：本文通过对自旋捕捉剂结构优化,研究其在细胞体系的应用。本文主要的研究进展 1、借助gaussian,优化分子结构,比较新型自旋捕捉剂反应前后键级、键长、二面角、前线轨道等参数,解释了为何间位取代的PBN加快能较快和甲基自由基反应。并通过比较各种自旋捕捉剂不同自由基自旋加合物的热力学能量,反应焓变,生成热,确定了较稳定的自旋捕捉剂结构,并通过实验验证了这种结构的稳定性。 2、电子顺磁共振(ESR)技术能够检测自旋捕捉剂自由基加合物的信号。但检测细胞内的自由基信号鲜有报道。本文利用ESR技术检测,用自旋捕捉剂BMPO捕捉了肿瘤细胞HepG2内的自由基,并通过模拟及实验的方法验证了该信号为超氧阴离子信号。 3、本文利用还原性荧光探针和氧化性荧光探针分别比较了肿瘤细胞HepG2和正常细胞L02两种细胞株的氧化还原水平,发现在HepG2细胞内的氧化性水平比L02细胞株高,而且还原水平HepG2细胞也同样要高。
[Abstract]:In this paper, the application of spin trapping agent in cell system was studied by optimizing its structure. The main research progress in this paper is as follows: 1. With the aid of gaussian, the molecular structure is optimized, and the parameters such as bond order, bond length, dihedral angle, frontier orbit and so on are compared before and after the reaction of the new spin trapping agent. It is explained why the meso-substituted PBN can accelerate the reaction with methyl radical. By comparing the thermodynamic energy, enthalpy change and heat of formation of different free radical spin adducts of various spin trapping agents, the stable spin trapping agent structure was determined. The stability of the structure is verified by experiments. 2. The electron paramagnetic resonance (ESR) technique can detect the signal of the free radical adducts of the spin trapping agent. However, the detection of free radical signals in cells is rarely reported. In this paper, the free radicals in HepG2 cells were captured by the spin trapping agent BMPO using ESR technique. The signal was proved to be superoxide anion signal by simulation and experiment. 3. In this paper, we compared the two kinds of tumor cells, HepG2 and L02, by reducing fluorescence probe and oxidizing fluorescence probe, respectively. The redox level of the strain, It was found that the level of oxidation in HepG2 cells was higher than that in L02 cells, and the reduction level of HepG2 cells was also higher than that of L02 cells.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R346

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