犬大脑中动脉近端栓塞建立类腔隙性脑梗死模型的研究

发布时间：2018-07-14 14:09

【摘要】：目的研究介入法血栓栓塞犬大脑中动脉(MCA)建立脑梗死模型的可行性、稳定性及急性期在磁共振扩散加权成像(DWI)上的演变过程,并探讨该模型的发生及其可能的发病机制。方法成年健康毕格犬6只,插管至左侧颈内动脉后采用1条直径约1.7 mm、长5 mm的白色血栓栓塞,栓塞成功后30 min~6 h内每30 min使用DWI序列连续扫描观察梗死病灶急性期的演变过程。栓塞后24 h、7天复查血管造影、MR扫描及神经功能评分,记录梗死灶的出现时间、位置及大小,分别比较6 h DWI病灶、24 h和7天的T2加权像上病灶的体积,并比较24 h与7天的神经功能评分。7天后处死动物取脑组织行病理检查。结果所有犬皆为左侧MCA的近端栓塞,DWI在栓塞后(1.24±0.51)h可以在基底节、脑白质区显示单一或数个异常信号病灶,6 h DWI病灶的直径为(6.90±2.08)mm。共有9个病灶在三个时间点都可以显示,病灶的大小均10 mm;栓塞后6 h DWI,24 h T2WI以及7 d T2WI病灶体积分别为(168.39±138.11)mm3、(186.88±161.54)mm3和(165.89±135.30)mm3,三者之间差异无明显统计学意义(P=0.0531)。7 d时动物均存活,24 h和7 d的神经功能评分分别为4.50±1.38和2.33±0.82(P=0.0009)。病理学检查证实为基底节梗死最常见,其次为脑白质区。结论介入单栓子栓塞法可以成功建立犬MCA近端栓塞的类腔隙性脑梗死模型,DWI能够在早期检测到小的脑缺血病灶。犬拥有丰富的颅内外血管吻合,近端大血管栓塞可形成类腔隙性脑梗死的病灶,因此栓塞可能是腔隙性脑梗死的病因之一。
[Abstract]:Objective to study the feasibility, stability and evolution process of interventional thromboembolism (MCA) on diffusion weighted magnetic resonance imaging (DWI) in the establishment of cerebral infarction model in dogs, and to explore the occurrence of the model and its possible pathogenesis. Methods six healthy adult Biger dogs were intubated to the left internal carotid artery with a white thromboembolism of about 1.7 mm in diameter and 5 mm in length. DWI sequence was used every 30 min after successful embolization to observe the course of acute stage of infarction. At 24 hours and 7 days after embolization, Mr scan and neurological function score were re-examined, the time, location and size of infarct were recorded, and the volume of lesions on T 2 weighted images at 24 h and 7 days on 6 h DWI were compared, respectively. The neurological function scores of 24 h and 7 d were compared. After 7 days, the animals were killed and the brain tissues were taken for pathological examination. Results in all dogs, the proximal DWI with left MCA could be seen in basal ganglion at (1.24 卤0.51) h after embolization, and the diameter of DWI was (6.90 卤2.08) mm on 6 h DWI with a single or several abnormal signal lesions in the white matter area. A total of 9 lesions can be displayed at all three points in time. At 6 h after embolization, the lesion volumes on 24 h T 2WI and 7 d T 2WI were (168.39 卤138.11) mm 3, (186.88 卤161.54) mm3 and (165.89 卤135.30) mm 3, respectively. There was no significant difference between the two groups at 24 h and 7 d after embolization (P0.0531). The neurological function scores were 4.50 卤1.38 and 2.33 卤0.82 on 24 h and 7 d after embolization, respectively. Pathological examination confirmed that basal ganglia infarction was the most common, followed by white matter area. Conclusion the model of lacunar infarction in dogs with proximal MCA embolization can be successfully established by single embolus embolization with interventional embolization. Small ischemic lesions can be detected in early stage on DWI. Dogs have abundant intracranial and external vascular anastomosis, and proximal large vessels embolization can form lacunar infarction lesions, so embolism may be one of the causes of lacunar cerebral infarction.
【作者单位】：南京医科大学第一附属医院放射科;
【分类号】：R743.3;R-332

【参考文献】