不同饮食结构构建小鼠非酒精性脂肪性肝炎模型的实验研究

发布时间：2018-07-15 18:05

【摘要】：目的采用高果糖、高脂、复合高果糖饲料构建小鼠非酒精性脂肪性肝炎模型,探讨最佳饮食结构。方法将60只C57BL/6J雄性小鼠随机分为高果糖组、高脂组、复合组和对照组,比较造模4、8和12周各组间一般情况、肝功能、脂质代谢、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)和病理学改变的差异。结果与对照组比较,造模组小鼠各时间点体质量、肝湿重和Lee's指数均明显升高(P0.01);12周时复合组体质量增长较高脂及高果糖组更明显(P0.05);H-E染色显示:造模组均有不同程度肝脏脂肪变,仅复合组肝小叶内见明显的炎症细胞浸润;血清检测结果显示:12周时复合组和高脂组总胆固醇、三酰甘油明显增高;复合组谷丙转氨酶(ALT)、谷草转氨酶(AST)、TNF-α、IL-6水平较高脂组、高果糖组升高明显。结论 12周的高脂、高糖饮食可成功构建适用于脂肪性肝炎研究的小鼠模型。
[Abstract]:Objective to establish the model of non-alcoholic fatty hepatitis in mice with high fructose, high fat and high fructose diet, and to explore the best diet structure. Methods 60 C57BL / 6J male mice were randomly divided into high fructose group, high fat group, compound group and control group. The differences of liver function, lipid metabolism, tumor necrosis factor- 伪 (TNF- 伪), interleukin-6 (IL-6) and pathological changes were compared. Results compared with the control group, the body weight, liver wet weight and Leehs index in the model group were significantly higher than those in the high fat group and high fructose group at 12 weeks (P0.01). The results of serum test showed that total cholesterol and triacylglycerol increased significantly in the compound group and hyperlipidemia group at 12 weeks, and the levels of alt, AST and TNF- 伪 IL-6 in the compound group were higher than those in the lipid group. High fructose group increased significantly. Conclusion 12 weeks of high fat and high sugar diet can be used to establish a mouse model suitable for the study of fatty hepatitis.
【作者单位】：苏州大学附属第一医院感染科;泰州市人民医院肝病科;
【基金】：国家“十二五”科技重大专项课题(2012ZX10002-004) 中国肝炎防治基金会天晴肝病研究基金(CFHPC20132110)~~
【分类号】：R575.5;R-332

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