青藤碱对痛风性关节炎模型大鼠TNF-α,IL-1β及COX-2的影响

发布时间：2018-07-28 15:19

【摘要】：目的:观察青藤碱对痛风性关节炎(GA)模型大鼠关节滑膜炎性细胞因子的影响,并进一步探讨其治疗GA的作用机制。方法:选用SD大鼠90只,随机选出10只作为正常组,其余80只采用尿酸钠诱导大鼠痛风性关节炎模型,于造模7 d后筛选出造模成功(关节炎指数大于4分)的大鼠共50只,随机分为模型组、双氯芬酸钾组、青藤碱低剂量组(25 mg/kg)、青藤碱中剂量组(50 mg/kg)、青藤碱高剂量组(100 mg/kg),每组10只;双氯芬酸钾组灌胃给予25 mg/kg双氯芬酸钾,青藤碱各剂量组分别在大鼠左后肢大腿上1/3外侧肌肉注射青藤碱注射液25,50,100 mg/kg,正常组、模型组大鼠同上述肌注方法注射1 ml/kg生理盐水,每天1次,连续给药21 d。观察GA大鼠踝关节肿胀程度,通过ELISA法测定各组大鼠滑膜悬液中TNF-α和IL-1β的含量,采用免疫组化检测大鼠滑膜组织COX-2的表达。结果:模型组大鼠踝关节肿胀程度随着时间推移逐渐增加,出现踝关节肿胀严重。与模型组比较,双氯芬酸钾组及青藤碱各剂量组关节肿胀明显抑制,差异有统计学意义(P㩳0.01)。双氯芬酸钾组及青藤碱各剂量组滑膜悬液中TNF-α、IL-1β、COX-2含量明显降低,与模型组比较,差异有统计学意义(P㩳0.01);与双氯芬酸钾组比较,青藤碱高剂量组大鼠滑膜悬液中IL-1β、TNF-α的含量有显著性差异(P㩳0.05);与双氯芬酸钾组比较,青藤碱各剂量组大鼠滑膜组织中COX-2表达略高,差异有统计学意义(P㩳0.05)。结论:青藤碱能有效降低GA大鼠滑膜中TNF-α、IL-1β及COX-2的水平,抑制炎性细胞因子的恶性循环,从而对GA起到治疗作用。
[Abstract]:Aim: to observe the effect of sinomenine on inflammatory cytokines in synovial membrane of gouty arthritis (GA) rats and to explore the mechanism of its therapeutic effect on GA. Methods: 90 Sprague-Dawley rats were selected randomly as normal group. The other 80 rats were induced by sodium uric acid to induce gouty arthritis. After 7 days of modeling, 50 rats were selected for successful modeling (arthritis index > 4 points). Model group, diclofenac potassium group, sinomenine low dose group (25 mg/kg), sinomenine middle dose group (50 mg/kg), sinomenine high dose group (100 mg/kg), 10 rats in each group, diclofenac potassium group was given 25 mg/kg diclofenac potassium intragastrally. Sinomenine was injected intramuscularly into the upper thigh of left hind limb of rats with sinomenine injection of 2550 mg / kg at 1 / 3 lateral side of thigh. Normal group and model group were injected with normal saline for 1 ml/kg once a day for 21 days. The degree of ankle swelling in GA rats was observed. The contents of TNF- 伪 and IL-1 尾 in synovial suspension of rats in each group were determined by ELISA method. The expression of COX-2 in synovial tissue of rats was detected by immunohistochemistry. Results: the swelling degree of ankle joint in the model group increased gradually with time, and the swelling of ankle joint was serious. Compared with the model group, the swelling of joints in diclofenac potassium group and sinomenine group was significantly inhibited (P0.01). The contents of TNF- 伪 -IL-1 尾 -COX-2 in synovial suspension of potassium diclofenac group and sinomenine group were significantly lower than those of model group (P0.01), and those of diclofenac potassium group were significantly lower than those of diclofenac potassium group (P0.01), and those of diclofenac potassium group were significantly lower than those of model group (P0.01). There was significant difference in the content of IL-1 尾 -TNF- 伪 in synovial suspension of rats with high dosage of sinomenine (P0. 05), and the expression of COX-2 in synovial tissue of sinomenine group was slightly higher than that of potassium diclofenac group (P0. 05). Conclusion: sinomenine can effectively reduce the levels of TNF- 伪 and IL-1 尾 and COX-2 in synovium of GA rats, inhibit the vicious circle of inflammatory cytokines, and thus play a therapeutic role in GA.
【作者单位】：柳州市中医院;
【基金】：广西卫生厅自筹计划科研课题(编号:Z2013663) 广西卫生厅自筹青年基金科研课题(编号:Z2014442)
【分类号】：R285.5;R-332

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