兔单侧输尿管可逆性不全梗阻模型制作及实验观察

发布时间：2018-07-29 15:27

【摘要】： 目的:建立可逆性输尿管部分梗阻(PUUO)动物模型,并观察部分梗阻及梗阻解除后肾脏功能及形态学改变。方法:成年健康雄性大白兔40只,用缝合线在左侧输尿管近肾盂处半缝扎,建立PUUO动物模型,分别于1、2、4、6、8月各随机抽取8只,行超声检查,然后将其中4只取双侧肾脏送病检,另外4只解除梗阻继续饲养,6个月后复查; 结果:梗阻后积水肾盂前后径(APD)、肾盂容积(V)逐渐增大,最大肾实质厚度(RCT)逐渐减小,与正常值比较有显著性差异( P 0.01) ,各时间段之间比较有显著性差异( P 0.01) ;梗阻解除后,APD、V逐渐变小,RCT逐渐增加,与梗阻解除前比较有显著性差异( P 0.01) ,时间段之间比较有显著性差异( P 0.01)。梗阻解除越早,恢复越快,病理学检查也支持这一结果。结论:该模型成功复制了PUUO所致肾脏形态学改变,是一种较理想的PUUO动物模型。
[Abstract]:Objective: to establish a reversible ureteral obstruction (PUUO) animal model and observe the renal function and morphology after partial obstruction and obstruction.
Methods: 40 adult healthy male rabbits were sutured with suture on the left ureter near the renal pelvis. The PUUO animal model was established. 8 rats were randomly selected at 1,2,4,6,8 month, respectively, and 4 of them were taken from bilateral kidney for examination, and the other 4 were reared for 6 months after 6 months.
Results: after the obstruction of the hydronephrosis (APD), the volume of renal pelvis (V) increased gradually and the maximum renal parenchyma thickness (RCT) gradually decreased, and there was a significant difference compared with the normal value (P 0.01). There was a significant difference between each time period (P 0.01). After the obstruction was relieved, APD, V gradually became smaller, RCT gradually increased, and was more significant than before the removal of obstruction. Differences (P 0.01), there was a significant difference between the time periods (P 0.01). The earlier the obstruction was relieved, the quicker the recovery. Pathological examination also supported the result.
Conclusion: the model successfully replicated the renal morphological changes induced by PUUO, and was an ideal PUUO animal model.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R-332;R693

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