非结核分枝杆菌MmpL家族同源性分析及拓扑结构预测

发布时间：2018-08-07 08:35

【摘要】：以非结核分枝杆菌中的胞分枝杆菌、鸟分枝杆菌和耻垢分枝杆菌三个标准株为研究对象,对其Mmp L家族蛋白的同源序列进行相似性分析和功能预测。根据NCBI数据库中分枝杆菌Mmp L蛋白序列进行多重序列比对;用TMHMM Server 2.0在线软件对Mmp L同源序列进行拓扑结构预测;用Interproscan对Mmp L3同源序列进行保守序列和结构域分析。同源分析结果显示:与H37Rv Mmp L家族比较,胞分枝杆菌中没有发现Mmp L2、Mmp L6、Mmp L7、Mmp L8、Mmp L9、Mmp L12和Mmp L13的同源序列;鸟分枝杆菌没有发现Mmp L6、Mmp L7、Mmp L8、Mmp L9、Mmp L12和Mmp L13的同源序列;耻垢分枝杆菌没有发现Mmp L2、Mmp L7、Mmp L9、Mmp L10、Mmp L12和Mmp L13的同源序列,推测可能与菌种差异性有关;拓扑结构预测发现大部分同源序列跨膜次数与H37Rv的对应Mmp L蛋白保持一致,少数序列与H37Rv的对应Mmp L蛋白有较小偏差;保守序列分析发现:与H37Rv比较三个标准株的Mmp L3同源序列有两个膜转运蛋白结构域(MMPL domain),没有固醇敏感多肽区(SSD domain)说明该同源序列有膜转运功能,无SSD介导的胆固醇自我平衡调节、物质运输以及细胞信号转导等功能。研究结果为进一步阐明非结核分枝杆菌的毒力、致病机制和疾病趋势提供基础资料。
[Abstract]:The homologous sequences of Mmp L family proteins from three standard strains of Mycobacterium spp., Mycobacterium avium and Mycobacterium smeareus were analyzed and their functions were predicted. Multiple sequence alignment was performed according to Mmp L protein sequence of Mycobacterium in NCBI database, topological structure of Mmp L homology sequence was predicted by TMHMM Server 2.0 online software, and conserved sequence and domain of Mmp L3 homology sequence were analyzed by Interproscan. The results of homology analysis showed that the homologous sequences of MMP L12 and MMP L12 of MMP L9, MMP L9, MMP L12 and MMP L13 were not found in Mycobacterium Mmp L2, MMP L6, MMP L6, MMP L9, MMP L12 and MMP L13 in Mycobacterium, and the homology of MMP L9 MMP L12 and MMP L13 were not found in Mycobacterium. The homologous sequences of MMP L9 and MMP L10 MMP L12 and Mmp L13 were not found in Mycobacterium smeareus, and the topological structure prediction revealed that most of the homologous sequences were consistent with the corresponding Mmp L protein of H37Rv, and the homologous sequences of MMP L7, MMP L9, MMP L10, MMP L12, MMP L12 and MMP L13 of MMP L9 were not found. There was a small deviation between a few sequences and the corresponding Mmp L protein of H37Rv. Conserved sequence analysis showed that there were two membrane transporter domains in Mmp L3 homologous sequence of three standard strains compared with H37Rv. There was no steroid-sensitive polypeptide region (SSD domain) in (MMPL domain), domain, which indicated that the homologous sequence had membrane transport function. No SSD-mediated self-regulation of cholesterol, substance transport and cell signal transduction. The results provide basic data for further elucidating the virulence, pathogenicity and disease trend of non-tuberculosis mycobacterium.
【作者单位】：武汉轻工大学生物与制药工程学院;
【基金】：武汉轻工大学研究生创新基金项目(2014cx019)
【分类号】：R378.91

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