胚胎骨髓来源的间充质干细胞对人T细胞免疫调节作用的研究
发布时间：2018-08-28 07:30
【摘要】： 第一部分胚胎骨髓来源的间充质干细胞对人T细胞免疫调节作用的研究 目的:研究胚胎骨髓来源的间充质干细胞(FBM-MSCs)对人T细胞的免疫调节作用。 方法:在FBM-MSCs与正常人来源的外周血单个核细胞(PBMCs)的共培养体系中,进行FBM-MSCs对PBMCs的增殖实验,分别从实时定量PCR,ELISA,FCM等方面观察FBM-MSCs对人Th17,Th1和Tregs细胞的调节作用。 结果:①在共培养体系中,FBM-MSCs可明显抑制人PBMCs的增殖(p＜0.05);②相对于PBMCs单独培养组,FBM-MSCs共培养组CD3+CD8-细胞比例上调(p＜0.05);③MSCs共培养组IL-17mRNA表达水平,上清IL-17水平和Th17细胞的数量明显升高(p＜0.05);④MSCs共培养组IFN-γmRNA表达水平和上清IFN-γ的蛋白水平明显减低(p＜0.05),同时,Th1和Tc1细胞数量也降低(p＜0.05);⑤FBM-MSCs可上调Foxp3 mRNA的表达水平(p＜0.05);⑥FBM-MSCs可上调IL-6 mRNA的水平(p＜0.05);⑦FBM-MSCs对IL-23的表达水平无明显影响(p＞0.05)。 结论:FBM-MSCs可促进人Th17和Tregs细胞的增殖,下调产IFN-γ的T细胞的产量。FBM-MSCs可能通过上调IL-6,而不是IL-23的表达而促进Th17细胞的增殖。 第二部分干扰素治疗肝炎病毒相关性血小板减少症 目的:评价干扰素(IFN-α)对肝炎病毒相关性血小板减少症患者的疗效。 方法:对20例肝炎病毒相关性血小板减少症患者进行IFN-α治疗,IFN-α具体 用法:3MU,皮下注射,一周两次,至少2月。 结果:20例患者中男性14例,女性6例,中位年龄36.5岁(7～50岁)。HBV阳性15例,HCV阳性4例,HBV和HCV同时阳性1例。所有患者均以血小板减少为首发表现,没有肝硬化、门静脉高压或脾脏明显增大的证据。9例患者获得完全反应(CR),6例部分反应(PR),2例次要反应(MR),CR率45%,总有效率85%,血小板反应持久。应用IFN-α治疗没有严重的不良反应。 结论:IFN-α是治疗肝炎病毒相关性血小板减少症切实有效的方法。
[Abstract]:The first part of the study on the immunomodulatory effect of mesenchymal stem cells derived from embryonic bone marrow on human T cells objective: to study the immunomodulatory effect of mesenchymal stem cells (FBM-MSCs) derived from embryonic bone marrow on human T cells. Methods: in the co-culture system of FBM-MSCs and (PBMCs) of peripheral blood mononuclear cells (PBMC) derived from normal people, the proliferation of FBM-MSCs to PBMCs was carried out, and the effect of FBM-MSCs on human Th17,Th1 and Tregs cells was observed from the aspects of real-time quantitative PCR,ELISA,FCM and so on. Results in the co-culture system, FBM-MSCs significantly inhibited the proliferation of human PBMCs (p < 0. 05). Compared with PBMCs alone, the proportion of CD3 CD8- cells increased (p < 0. 05) and the expression level of IL-17mRNA was up-regulated in FBM-MSCs co-cultured group (p < 0. 05). The expression level of IFN- 纬 mRNA and the protein level of IFN- 纬 in supernatant were significantly decreased (p < 0. 05), and the number of Th1 and Tc1 cells were also decreased (p < 0. 05). 5FBM-MSCs could upregulate the surface of Foxp3 mRNA. (P < 0. 05) 6FBM-MSCs upregulated the level of IL-6 mRNA (p < 0. 05). 7FBM-MSCs had no significant effect on the expression of IL-23 (p > 0. 05). Conclusion: FBM-MSCs can promote the proliferation of human Th17 and Tregs cells, and down-regulate the production of IFN- 纬 -producing T cells. FBM-MSCs may promote the proliferation of Th17 cells by up-regulating the expression of IL-6, rather than IL-23. Part two: interferon in the treatment of hepatitis virus associated thrombocytopenia objective: to evaluate the efficacy of interferon (IFN- 伪) in patients with hepatitis virus associated thrombocytopenia. Methods: 20 patients with hepatitis virus associated thrombocytopenia were treated with IFN- 伪. Results among the 20 patients, 14 were male and 6 were female. The median age was 36.5 years old (750 years) .15 cases were positive for HCV and 4 cases were positive for both HBV and HCV. Thrombocytopenia was the first manifestation in all patients. There was no evidence of cirrhosis, portal hypertension or splenic enlargement in 9 patients. Complete response (CR) was obtained in 6 cases, partial response (PR) in 2 cases, (MR) CR rate in 2 cases, total effective rate 85%, platelet reaction lasting. There was no serious adverse reaction in the treatment of IFN- 伪. ConclusionTwo-IFN- 伪 is an effective and effective method for the treatment of hepatitis virus associated thrombocytopenia.
【学位授予单位】：中国协和医科大学
【学位级别】：博士
【学位授予年份】：2008
【分类号】：R392
本文编号：2208710
[Abstract]:The first part of the study on the immunomodulatory effect of mesenchymal stem cells derived from embryonic bone marrow on human T cells objective: to study the immunomodulatory effect of mesenchymal stem cells (FBM-MSCs) derived from embryonic bone marrow on human T cells. Methods: in the co-culture system of FBM-MSCs and (PBMCs) of peripheral blood mononuclear cells (PBMC) derived from normal people, the proliferation of FBM-MSCs to PBMCs was carried out, and the effect of FBM-MSCs on human Th17,Th1 and Tregs cells was observed from the aspects of real-time quantitative PCR,ELISA,FCM and so on. Results in the co-culture system, FBM-MSCs significantly inhibited the proliferation of human PBMCs (p < 0. 05). Compared with PBMCs alone, the proportion of CD3 CD8- cells increased (p < 0. 05) and the expression level of IL-17mRNA was up-regulated in FBM-MSCs co-cultured group (p < 0. 05). The expression level of IFN- 纬 mRNA and the protein level of IFN- 纬 in supernatant were significantly decreased (p < 0. 05), and the number of Th1 and Tc1 cells were also decreased (p < 0. 05). 5FBM-MSCs could upregulate the surface of Foxp3 mRNA. (P < 0. 05) 6FBM-MSCs upregulated the level of IL-6 mRNA (p < 0. 05). 7FBM-MSCs had no significant effect on the expression of IL-23 (p > 0. 05). Conclusion: FBM-MSCs can promote the proliferation of human Th17 and Tregs cells, and down-regulate the production of IFN- 纬 -producing T cells. FBM-MSCs may promote the proliferation of Th17 cells by up-regulating the expression of IL-6, rather than IL-23. Part two: interferon in the treatment of hepatitis virus associated thrombocytopenia objective: to evaluate the efficacy of interferon (IFN- 伪) in patients with hepatitis virus associated thrombocytopenia. Methods: 20 patients with hepatitis virus associated thrombocytopenia were treated with IFN- 伪. Results among the 20 patients, 14 were male and 6 were female. The median age was 36.5 years old (750 years) .15 cases were positive for HCV and 4 cases were positive for both HBV and HCV. Thrombocytopenia was the first manifestation in all patients. There was no evidence of cirrhosis, portal hypertension or splenic enlargement in 9 patients. Complete response (CR) was obtained in 6 cases, partial response (PR) in 2 cases, (MR) CR rate in 2 cases, total effective rate 85%, platelet reaction lasting. There was no serious adverse reaction in the treatment of IFN- 伪. ConclusionTwo-IFN- 伪 is an effective and effective method for the treatment of hepatitis virus associated thrombocytopenia.
【学位授予单位】：中国协和医科大学
【学位级别】：博士
【学位授予年份】：2008
【分类号】：R392
【参考文献】
相关期刊论文 前1条
1 曹兰;王曦;谢俊丽;;HCV和HIV感染并发血小板减少性紫癜1例[J];临床血液学杂志;2006年03期
，本文编号：2208710
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