大鼠骨移植癌痛模型的建立及其机制的初步研究
发布时间：2018-08-30 10:41
【摘要】： 癌症痛严重影响病人生活质量,其机制研究刚刚起步。本工作参照文献,通过测定模型大鼠体重,胫骨病理。胫骨X光片,后肢热痛觉过敏,机械性痛觉超敏(触诱发痛),自发性疼痛等综合评估方法建立癌症实验动物模型。并运用免疫组化等方法,观察癌痛时大鼠脊髓背角胶质细胞功能活动变化,探讨胶质细胞在癌痛中的作用。结果提示胫骨内接种walker256细胞可产生多因素依赖的疼痛行为学改变,并与临床表现相吻合,适合作为癌症性疼痛动物模型用于癌症性疼痛的机制及治疗研究;大鼠胫骨癌痛时,肿瘤细胞接种对侧后肢也出现机械性痛觉超敏,提示该模型可能存在镜像痛;癌痛时双侧脊髓背角星型胶质细胞和小胶质细胞的激活是双侧的,提示可能与镜像痛有关,并可能参与癌痛的过程,但是脊髓胶质细胞在癌痛中的确切作用还有待于进一步深入研究。
[Abstract]:Cancer pain seriously affects the quality of life of patients, its mechanism research has just started. In this work, the body weight and tibia pathology of the model rats were measured with reference to the literature. The animal model of cancer was established by comprehensive evaluation methods of tibia X-ray, thermal hyperalgesia in hind limbs, mechanical hyperalgesia (touch-induced pain) and spontaneous pain. The functional changes of glial cells in the spinal dorsal horn of rats were observed by immunohistochemical method, and the role of glial cells in cancer pain was discussed. The results suggest that walker256 cells inoculated in the tibia can produce multifactor dependent pain behavior changes, which are consistent with the clinical manifestations, and are suitable for the mechanism and treatment of cancer pain as an animal model of cancer pain. Mechanical pain hypersensitivity was also found in the contralateral hindlimb inoculated with tumor cells, suggesting that there might be mirror pain in the model, and that the activation of astrocytes and microglia in the dorsal horn of the spinal cord was bilateral, suggesting that it might be related to mirror pain. And may be involved in the process of cancer pain, but the exact role of spinal glial cells in cancer pain needs further study.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R-332;R73-3
本文编号：2212826
[Abstract]:Cancer pain seriously affects the quality of life of patients, its mechanism research has just started. In this work, the body weight and tibia pathology of the model rats were measured with reference to the literature. The animal model of cancer was established by comprehensive evaluation methods of tibia X-ray, thermal hyperalgesia in hind limbs, mechanical hyperalgesia (touch-induced pain) and spontaneous pain. The functional changes of glial cells in the spinal dorsal horn of rats were observed by immunohistochemical method, and the role of glial cells in cancer pain was discussed. The results suggest that walker256 cells inoculated in the tibia can produce multifactor dependent pain behavior changes, which are consistent with the clinical manifestations, and are suitable for the mechanism and treatment of cancer pain as an animal model of cancer pain. Mechanical pain hypersensitivity was also found in the contralateral hindlimb inoculated with tumor cells, suggesting that there might be mirror pain in the model, and that the activation of astrocytes and microglia in the dorsal horn of the spinal cord was bilateral, suggesting that it might be related to mirror pain. And may be involved in the process of cancer pain, but the exact role of spinal glial cells in cancer pain needs further study.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R-332;R73-3
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