高脂饮食喂养肥胖模型小鼠的构建:分析营养因素和代谢综合征的关系

发布时间：2018-08-30 14:38

【摘要】：背景:代谢综合征对人体的危害极大,且受到多种因素的影响。通过构建饮食诱导动物模型,可以更好的分析营养因素和代谢综合征之间的关系,为临床治疗等提供可靠的参考依据。目的:构建高脂饮食喂养肥胖小鼠模型,探讨营养因素和代谢综合征的关系。方法:选择30只小鼠随机分为模型组20只和对照组10只,分别予以高脂饮食纯化饲料和普通饲料喂养,连续喂养10周。结果与结论:与对照组相比,高脂饮食纯化饲料喂养后1周,模型组小鼠即出现体质量升高,且随着喂养时间增加呈现出差异逐渐增大的情况;喂养后8周,模型组的体质量指数和显著升高(P0.05)。喂养后4周,模型组的空腹静脉全血血糖即显著升高,且随着喂养时间的增长呈现出逐渐上升的情况;喂养后5周,模型组的空腹胰岛素水平也开始出现升高;经口服糖耐量实验,随着喂养时间的增长,模型组小鼠表现出糖耐量逐渐下降的趋势;喂养后8周,模型组的血清总胆固醇、高密度脂蛋白胆固醇水平均出现显著性上升高(P0.05);喂养后10周,模型组的三酰甘油、血清总胆固醇、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇均升高(P0.05)。结果证实,实验成功构建了高脂饮食喂养肥胖小鼠模型,可能模拟人类的代谢综合征的自然发病过程,且营养因素与代谢综合征密切相关。
[Abstract]:Background: metabolic syndrome is harmful to human body and affected by many factors. The relationship between nutritional factors and metabolic syndrome can be better analyzed by establishing animal model induced by diet and providing reliable reference for clinical treatment. Objective: to study the relationship between nutritional factors and metabolic syndrome in obese mice fed with high fat diet. Methods: thirty mice were randomly divided into model group (n = 20) and control group (n = 10). Results and conclusion: compared with the control group, the body mass of mice in the model group increased 1 week after feeding with high fat diet purified feed, and the difference gradually increased with the increase of feeding time, and at 8 weeks after feeding, the body mass of the mice in the model group increased with the increase of the feeding time. The body mass index and body mass index of the model group were significantly increased (P0.05). 4 weeks after feeding, the fasting whole blood glucose of the model group increased significantly, and gradually increased with the increase of feeding time, and the fasting insulin level of the model group began to increase 5 weeks after feeding. After oral glucose tolerance test, with the increase of feeding time, the model group mice showed a decreasing trend of glucose tolerance, and 8 weeks after feeding, the serum total cholesterol of model group, The level of high density lipoprotein cholesterol increased significantly (P0.05). Ten weeks after feeding, the level of triacylglycerol, serum total cholesterol, low density lipoprotein cholesterol and high density lipoprotein cholesterol in model group were all increased (P0.05). The results showed that the obese mice model of high fat diet feeding was successfully constructed, which may mimic the natural pathogenesis of human metabolic syndrome, and the nutritional factors are closely related to the metabolic syndrome.
【作者单位】：辽河油田总医院干诊科;辽河油田总医院手足外科;辽河油田总医院神经外科;中国医科大学附属第一医院老年医学科;
【分类号】：R589.2;R-332

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