Micro-CT和力学测试观察环磷酰胺诱导大鼠骨质疏松模型的时效特点

发布时间：2018-09-09 11:02

【摘要】：目的应用骨生物力学测试和micro-CT技术,观察环磷酰胺诱导大鼠骨质疏松的时效关系。方法 3月龄SPF级雄性SD大鼠用环磷酰胺4.5 mg·kg-1·d-1连续灌胃15 d,停药后的0、15和30 d,取右侧股骨进行生物力学检测,然后进行MicroCT扫描及三维重建。结果大鼠环磷酰胺诱导15 d后,股骨松质骨的三维结构和评价参数均呈现明显的微观结构退化,小梁骨结构由板状向杆状变化,而生物力学性能降低,骨脆性增加,骨折风险增加。停药后15d和30d,股骨松质骨的三维结构和评价参数仍然呈现明显的骨质疏松变化,除韧性系数和刚性系数外,其他生物力学参数仍然低下。停药后,环磷酰胺组大鼠以上各骨评价参数虽呈现了稳定的增长趋势,但较正常大鼠明显减弱。结论环磷酰胺诱导的大鼠骨质疏松模型近期效果好,停药后1个月内,骨增长的抑制效果虽有所减弱,但骨丢失仍然明显。
[Abstract]:Objective to investigate the effect of cyclophosphamide on osteoporosis in rats by bone biomechanical test and micro-CT technique. Methods 3-month-old SPF male SD rats were administrated with cyclophosphamide 4.5 mg kg-1 d-1 for 15 days. The right femur was taken for biomechanical examination at 15 and 30 days after withdrawal. Then MicroCT scan and 3D reconstruction were performed. Results after 15 days of cyclophosphamide induction, the three-dimensional structure and evaluation parameters of femur cancellous bone showed obvious degeneration, trabecular bone structure changed from plate to rod, biomechanical properties decreased and bone brittleness increased. Increased risk of fracture. The three-dimensional structure and evaluation parameters of cancellous bone of femur still showed obvious changes in osteoporosis at 15 and 30 days after withdrawal, except for toughness coefficient and rigidity coefficient, other biomechanical parameters were still low. After withdrawal, the bone evaluation parameters of cyclophosphamide group showed a steady increasing trend, but decreased significantly compared with normal rats. Conclusion the model of osteoporosis induced by cyclophosphamide has a good short-term effect. The inhibitory effect of bone growth is weakened but bone loss is still obvious within one month after withdrawal of cyclophosphamide.
【作者单位】：广东医学院药学院;
【基金】：国家自然科学基金资助项目(No 81273518) 湛江市科技计划项目(2012C3104017);湛江市科技计划项目(2007C07003)
【分类号】：R580;R-332

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