猪胰腺直接注射与介入注射磁流体的安全性实验研究

发布时间：2018-09-09 14:50

【摘要】： 目的:观察Fe_3O_4磁流体(magnetic fluid,MF)注射到猪胰腺后的安全性,并探索采用动脉介入的方法向猪胰腺注射Fe_3O_4磁流体的可行性。方法:1.Fe_3O_4磁流体经手术直接局部注射到猪的胰腺,注射前后测量并对比实验动物血淀粉酶、空腹血糖、血常规值的变化;磁流体注射6天后观察胰腺及其他内脏器官磁流体的分布及病理变化。2.对猪胰腺进行解剖学观察并绘制猪胰腺血管图,采用seldinger法经股动将sp管插到脾动脉,向胰腺供血动脉注射浓度为100mg/ml的Fe_3O_4磁流体1ml。磁流体注射6天后处死实验动物,取胰腺及其他内脏器官行组织切片HE染色。结果:1.磁流体注射前后实验动物血淀粉酶、空腹血糖、血常规值均无明显变化,磁流体沉积于注射部位,磁流体周围胰腺组织有巨噬细胞浸润,其他脏器没有明显磁流体分布,组织结构正常。2.实验成功绘制了胰腺血管供应图,通过动脉介入的方式成功向胰腺内注射磁流体,经病检显示磁流体较好地分布于注射部位胰腺内,其他脏器未见明显的磁流体分布。结论:通过对实验动物血淀粉酶、空腹血糖、血常规等指标的检测可以观察到Fe_3O_4磁流体胰腺注射是安全可行的。我们成功绘制了胰腺动脉血管图,将为人类胰腺疾病的猪模型实验研究提供参考。实验结果显示采用动脉介入的方式向胰腺内注射磁流体是可行的,且没有引起胰腺组织的明显损伤,这一技术可用于进一步的胰腺癌磁靶向热疗研究。
[Abstract]:Aim: to observe the safety of Fe_3O_4 magnetic fluid (magnetic fluid,MF) injection into porcine pancreas and to explore the feasibility of injecting Fe_3O_4 magnetic fluid into porcine pancreas by arterial intervention. Methods 1. Fe3O4 magnetic fluid was injected directly into pig pancreas after operation. The changes of serum amylase, fasting blood glucose and blood routine were measured and compared before and after injection. The distribution and pathological changes of magnetic fluid in pancreas and other visceral organs were observed 6 days after MHD injection. The porcine pancreas was anatomically observed and the porcine pancreatic angiogram was drawn. The sp tube was inserted into the splenic artery by seldinger method and the Fe_3O_4 magnetic fluid containing 100mg/ml was injected into the pancreatic artery. The animals were killed 6 days after magnetic fluid injection. The pancreas and other visceral organs were taken for HE staining. The result is 1: 1. Before and after magnetic fluid injection, there were no significant changes in serum amylase, fasting blood glucose, blood routine value, magnetic fluid deposition in the injection site, infiltration of macrophages in pancreatic tissue around magnetic fluid, and no obvious distribution of magnetic fluid in other organs. Normal structure. The pancreatic vascular supply map was successfully drawn, and the magnetic fluid was injected into the pancreas successfully by arterial intervention. The pathological examination showed that the magnetic fluid was well distributed in the pancreas at the injection site, but there was no obvious magnetic fluid distribution in other organs. Conclusion: it is safe and feasible to observe Fe_3O_4 magnetic fluid pancreatic injection by detecting serum amylase, fasting blood glucose and blood routine. We successfully mapped the pancreatic artery, which will provide reference for the pig model study of human pancreatic disease. The experimental results show that it is feasible to inject magnetic fluid into the pancreas by arterial intervention without causing obvious damage to pancreatic tissue. This technique can be used in the further study of magnetic targeted hyperthermia of pancreatic cancer.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R735.9;R312

【参考文献】