角质细胞生长因子对γ-射线辐射损伤小鼠的防护作用及其作用机理初步研究

发布时间：2018-09-12 12:21

【摘要】： 目的:探讨KGF对60Coγ-射线辐射损伤小鼠的防护作用及其作用机理。方法:本试验中,我们以昆明种小鼠构建动物模型,60只小鼠随机分为60Co模型组、KGF低剂量组、KGF中剂量组、KGF高剂量组以及正常对照组和阳性对照组(京尼平苷),在相同条件下饲养7天,模型组、KGF组和京尼平苷组小鼠给予6.0 Gy 60Co-γ(1.0 Gy/min)射线一次性整体照射,5天后处死动物。分别在实验开始时、试验后第7天、辐照后第5天观察各组小鼠体重的变化;辐照后5天处死动物,计算小鼠的胸腺指数和脾脏指数;观察肝、肺及肠的组织切片;测定肝、肺组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)、谷胱甘肽硫转移酶(GST)和丙二醛(MDA)的含量以及总抗氧化能力( T-AOC)指标;测定小鼠空肠绒毛长度变化。结果: 1)KGF可以有效改善辐射引起的小鼠饮食和体重的下降; 2)与对照组相比,KGF可以显著提高小鼠的胸腺系数、脾脏系数,减轻辐射对小鼠免疫系统的危害; 3)KGF对辐射引起的小鼠肝、肺及肠损伤有较明显的保护作用; 4)KGF可以抑制辐射引起的肝、肺组织中SOD、GSH-px、GST和T-AOC活性,提高MDA活性,并具有一定的剂量依赖性; 5)与60Co模型组相比,用KGF处理辐照小鼠,空肠绒毛长度明显较长。结论KGF能对γ-射线诱导的小鼠损伤起防护作用,其机制可能与提高机体免疫功能,调节抗氧化酶含量有关
[Abstract]:Objective: to investigate the protective effect and mechanism of KGF on 60Co 纬-ray irradiated mice. Methods: in this experiment, 60 mice were randomly divided into 60Co model group, low dose group, middle dose group, high dose group, normal control group and positive control group (geniposide). The mice were fed under the same conditions for 7 days. The mice in the model group and geniposide group were given 6.0 Gy 60 Co- 纬 (1.0 Gy/min) irradiation for 5 days. At the beginning of the experiment, on the 7th day after the experiment and on the fifth day after irradiation, the weight changes of the mice in each group were observed; the animals were killed on the 5th day after irradiation to calculate the thymus index and spleen index of the mice; the liver, lung and intestine sections were observed; and the liver was measured. The contents of superoxide dismutase (SOD), glutathione peroxidase (GSH-px), glutathione S-transferase (GST), malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in lung tissue were measured. Results: 1) KGF could effectively improve the diet and body weight loss of mice induced by radiation, 2) compared with the control group, KGF could significantly increase the thymus coefficient and spleen coefficient of mice. KGF has obvious protective effect on liver, lung and intestine injury induced by radiation. 4) KGF can inhibit the activities of SOD,GSH-px,GST and T-AOC in liver and lung tissue induced by radiation. The activity of MDA was increased in a dose-dependent manner. 5) compared with the model group of 60Co, the length of jejunum villi was longer when treated with KGF. Conclusion KGF can protect mice against 纬 -ray induced injury, and its mechanism may be related to the improvement of immune function and the regulation of antioxidant enzyme content.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R363

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