体外诱导骨髓与脐血间充质干细胞向成骨细胞分化及其成骨活性的比较

发布时间：2018-09-14 09:27

【摘要】：背景:脐血与骨髓来源的间充质干细胞在一定诱导条件作用下均具有多向分化的能力。目的:比较脐血与骨髓来源间充质干细胞向成骨细胞诱导分化能力的差异。方法:采用密度梯度法分离培养人脐血间充质干细胞和骨髓间充质干细胞,当细胞汇合90%后胰蛋白酶消化传代,取第3代脐血间充质干细胞和骨髓间充质干细胞,以细胞密度为8×104/孔接种,当细胞达80%融合时,加入含体积分数为10%胎牛血清、0.1μmol/L地塞米松、50μmol/L维生素C、10 mmol/Lβ-甘油磷酸钠的低糖型DMEM成骨细胞诱导液培养。结果与结论:两种来源间充质干细胞的形态和生物学特性无明显差异,细胞表面均强表达CD44,CD29,不表达CD34;两者均具有向成骨细胞诱导分化的潜能,稳定表达了成骨细胞标志性的产物碱性磷酸酶、骨矿化结节,而且两种细胞的成骨活性差异无显著性意义。结果表明脐血和成人骨髓来源间充质干细胞向成骨细胞诱导分化能力相似,均可作为骨组织工程的种子细胞。
[Abstract]:Background: mesenchymal stem cells (MSCs) derived from cord blood and bone marrow have the ability of multi-differentiation under certain induction conditions. Aim: to compare the differentiation ability of mesenchymal stem cells from cord blood and bone marrow into osteoblasts. Methods: human umbilical cord blood mesenchymal stem cells and bone marrow mesenchymal stem cells were isolated and cultured by density gradient method. When the cell density was 8 脳 10 ~ 4 / well, when the cells were fused to 80%, the low sugar type DMEM osteoblast was cultured with 0.1 渭 mol/L dexamethasone 50 渭 mol/L vitamin C and 10 mmol/L 尾 -glycerophosphate sodium in fetal bovine serum containing 10% volume fraction. Results and conclusion: there was no significant difference in morphology and biological characteristics between the two mesenchymal stem cells. Both of them had the potential of inducing differentiation into osteoblasts by strongly expressing CD44,CD29, and not expressing CD34; on the surface of the two kinds of mesenchymal stem cells. Stable expression of osteoblast iconic product alkaline phosphatase, bone mineralized nodules, and there is no significant difference in osteogenic activity between the two kinds of cells. The results showed that cord blood and adult bone marrow-derived mesenchymal stem cells could be used as seed cells for bone tissue engineering.
【作者单位】：青岛科技大学;青岛市第九人民医院内分泌科;青岛大学附属医院内分泌科;
【分类号】：R329.2

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