应用表皮干细胞、间充质干细胞构建复合皮肤及移植实验

发布时间：2018-10-08 12:15

【摘要】：目的探讨应用表皮干细胞、间充质干细胞构建复合皮肤修复全层缺损皮肤的可行性。方法选择新西兰大白兔18只,在每只兔背上用常规手术方法切去3个大小相同的全层皮肤缺损创面,面积约2 cm×2 cm。将经处理的脱细胞真皮(acellular dermal matrix,ADM)接种骨髓间充质干细胞(mesenchymal stemcells,MSCs)和表皮干细胞(epidermal stemcells,ESCs)制成复合皮肤。实验分A、B、C3组,A组移植复合皮肤;B组移植脱细胞真皮;C组用油纱布敷盖创面。观察各组移植后1~3周内创面愈合、肉芽组织生长及上皮化等情况,并进行创面组织HE染色。结果术后A、B、C3组创面愈合时间分别为(14.6±1.1)、(18.3±1.2)、(23.0±1.4)d,组间比较差异有统计学意义(P0.05)。术后14 d创面标本HE染色观察:A组创面已上皮化,毛细血管、成纤维细胞数量增加,胶原合成增多,肉芽组织形成丰富;B、C组创面上皮化不全,仍可见许多炎性细胞,肉芽组织中毛细血管、成纤维细胞数量不如A组。结论表皮干细胞、间充质干细胞接种于脱细胞真皮构建复合皮肤,可加速新生血管形成及创面上皮化,可用于全层皮肤缺损创面的治疗,提高创面愈合质量。
[Abstract]:Objective to investigate the feasibility of using epidermal stem cells and mesenchymal stem cells to construct composite skin to repair skin defect. Methods A total of 18 New Zealand white rabbits were selected and 3 full-thickness skin defect wounds of the same size were removed by conventional surgical method on the back of each rabbit, with an area of about 2 cm 脳 2 cm.. The treated acellular dermis (acellular dermal matrix,ADM was inoculated with bone marrow mesenchymal stem cells (mesenchymal stemcells,MSCs) and epidermal stem cells (epidermal stemcells,ESCs) to produce composite skin. The wounds were covered with oil gauze in group A and group B after transplantation of acellular dermis in group A and group B respectively. Wound healing, granulation tissue growth and epithelization were observed within 1 to 3 weeks after transplantation, and HE staining of wound tissue was performed. Results the wound healing time was (14.6 卤1.1), () 18.3 卤1.2), (23.0 卤1.4) d in group A (P 0.05). On the 14th day after operation, HE staining of wound specimens showed that the wound in group A was epithelialized, the number of capillary and fibroblasts increased, the synthesis of collagen was increased, and the granulation tissue was rich in the formation of granulation tissue. In group C, many inflammatory cells could still be seen. The number of capillary and fibroblasts in granulation tissue was lower than that in group A. Conclusion Epidermal stem cells and mesenchymal stem cells inoculated into acellular dermis to construct composite skin can accelerate the formation of neovascularization and wound epithelization and can be used in the treatment of full-thickness skin defect wounds and improve the quality of wound healing.
【作者单位】：南昌大学第一附属医院烧伤科;
【分类号】：R318

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