西罗莫司促进小鼠心脏移植模型中调节性T细胞的分化和增殖

发布时间：2018-10-23 06:38

【摘要】：目的探讨西罗莫司(SRL)对小鼠异位心脏移植模型的移植物存活时间以及脾脏中调节性T细胞(Treg)分化和增殖的影响。方法应用Cuff法建立雄性BALB/c→C57BL/6小鼠颈部异位心脏移植模型。术后随机分为3组各10只受体:对照组术后不予特殊药物治疗,SRL组术后1~14 d予SRL 10 mg/(kg·d)灌胃,环孢素(Cs A)组术后1~14 d予Cs A 30 mg/(kg·d)灌胃。记录移植心脏存活时间,于移植心停搏或术后14 d取脾,分离单个核细胞,上流式细胞仪检测CD4+CD25+Treg占CD4+T细胞比例(CD4+CD25+Treg%),采用逆转录-聚合酶链反应(RT-PCR)法半定量检测Foxp3信使核糖核酸(mRNA)表达水平。结果与对照组比较,Cs A组和SRL组均明显延长小鼠移植心的生存时间(均为P0.01),而两者之间比较差异无统计学意义(P0.05)。与对照组比较,Cs A组脾脏中CD4+CD25+Treg%明显降低,而SRL组则明显升高(均为P0.01),后两组比较差异有统计学意义(P0.01)。SRL组脾脏T细胞的Foxp3 mRNA表达明显高于对照组和Cs A组,其中对照组亦明显高于Cs A组(均为P0.01)。结论在小鼠心脏移植模型中,SRL明显延长移植物的存活期,促进CD4+CD25+Treg的增殖与生长,有利于免疫耐受的形成。
[Abstract]:Objective to investigate the effects of sirolimus (SRL) on graft survival time and (Treg) differentiation and proliferation of regulatory T cells in the spleen of mice with heterotopic heart transplantation. Methods the cervical heterotopic heart transplantation model of male BALB/c C57BL/6 mice was established by Cuff method. Each group was randomly divided into 3 groups: the control group received no special drug therapy, the SRL group received SRL 10 mg/ (kg d) intragastric perfusion on the 14th day after operation, and the cyclosporine (Cs A) group received Cs A30 mg/ (kg d) intragastric perfusion on the 1th day after operation. The survival time of the transplanted heart was recorded, and the spleen was taken 14 days after cardiac arrest, and the mononuclear cells were isolated. The ratio of CD4 CD25 Treg to CD4 T cells (CD4 CD25 Treg%) was detected by upflow cytometry, and the (mRNA) expression of Foxp3 messenger RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results compared with the control group, Cs A group and SRL group significantly prolonged the survival time of heart transplantation in mice (P0.01), but there was no significant difference between the two groups (P0.05). Compared with the control group, CD4 CD25 Treg% in the spleen of the, Cs A group was significantly lower than that in the control group, while that in the SRL group was significantly higher than that in the control group (all P0.01). There was a significant difference between the latter two groups (P0.01). SRL group was significantly higher than the control group and Cs A group in Foxp3 mRNA expression of spleen T cells. The control group was also significantly higher than Cs A group (P0.01). Conclusion in mouse heart transplantation model, SRL significantly prolonged the survival period of the graft, promoted the proliferation and growth of CD4 CD25 Treg, and was beneficial to the formation of immune tolerance.
【作者单位】：北京海军总医院普外科;
【分类号】：R-332;R654.2

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