乙酰胆碱（ACh）对离体人心房肌电生理特性的影响及其脱敏机制研究
发布时间：2018-10-23 11:46
【摘要】： 实验一乙酰胆碱(ACh)对离体人心房肌电生理特性的影响 目的:研究迷走神经递质乙酰胆碱(ACh)对离体人心房肌电生理特性的影响 方法:选择20例先天性心脏病手术患者,手术中切取患者右心耳组织,采用标准玻璃微电极技术及张力换能器,检测灌注乙酰胆碱(ACh)前后患者右心房细胞静息膜电位(RMP),动作电位达90%复极的时程(APD90),自发动作电位及收缩力的改变。 结果:乙酰胆碱能显著提高人心房肌肉静息电位(RMP)(54.7±2.19mv vs 61.4±1.8mv),显著缩短APD90(406.3±14.61ms vs 316.2±15.40ms),P0.05。乙酰胆碱能明显抑制人心房肌自发动作电位及收缩力(60.5±3.2次/分vs 0次/分),P0.05。并且以上效应随时间变化逐渐减弱。 结论:乙酰胆碱能显著提高人心房肌静息电位和缩短动作电位时程,明显抑制人心房肌自发动作电位及收缩力,并且以上效应存在脱敏现象。 实验二乙酰胆碱(ACh)对人心房肌作用的脱敏机制研究 目的:研究迷走神经递质乙酰胆碱(ACh)对离体人心房肌作用的脱敏机制 方法:选择20例先天性心脏病手术患者,手术中切取患者右心耳组织,采用全细胞膜片钳技术及Western Blotting免疫印迹技术分别记录人心房肌细胞IK,Ach通道电流密度变化以及灌流前后心房肌组织Kir3.4蛋白质的表达。 结果:乙酰胆碱能激活人心房肌细胞上的IK,Ach通道产生电流,而且此电流存在明显的脱敏现象。灌流前后心房肌组织Kir3.4蛋白质的表达无明显差异。 结论:乙酰胆碱对人心房肌作用的脱敏现象可能与人心房肌IK,Ach通道电流的脱敏有关,而与IK,Ach通道蛋白表达无明显关系。
[Abstract]:Effect of acetylcholine (ACh) on electrophysiological characteristics of isolated human atrial myography objective: to study the effect of vagus neurotransmitter acetylcholine (ACh) on the electrophysiological characteristics of human atrial myograph in vitro Methods: twenty patients with congenital heart disease were selected. The tissue of the right atrial appendage was removed during the operation, and the standard glass microelectrode technique and tension transducer were used. The time course (APD90) of resting membrane potential (RMP),) of right atrial cells before and after perfusion of acetylcholine (ACh) to 90% repolarization and the changes of spontaneous action potential and contractility were measured. Results: acetylcholine significantly increased the resting potential of human atrial muscle (RMP) (54.7 卤2.19mv vs 61.4 卤1.8mv), shortened APD90 (406.3 卤14.61ms vs 316.2 卤15.40ms), and significantly shortened APD90 (P 0.05). Acetylcholine could significantly inhibit the spontaneous action potential and contractility of human atrial muscle (60.5 卤3.2 times / min vs 0 times / min, P 0.05). And the above effects gradually weaken with time. Conclusion: acetylcholine can significantly increase the resting potential of human atrial muscle and shorten the duration of action potential, inhibit the spontaneous action potential and contractility of human atrial muscle, and the above effects have desensitization phenomenon. Desensitization of human atrial muscle by diacetylcholine (ACh) objective: to study the desensitization mechanism of vagus neurotransmitter acetylcholine (ACh) on human atrial muscle in vitro Methods: twenty patients with congenital heart disease were selected. The right atrial appendage tissue was removed. The changes of IK,Ach channel current density in human atrial myocytes and the expression of Kir3.4 protein in human atrial myocytes before and after perfusion were recorded by whole-cell patch clamp technique and Western Blotting Western blotting technique. Results: acetylcholine could activate the IK,Ach channel in human atrial myocytes, and this current had obvious desensitization phenomenon. There was no significant difference in the expression of Kir3.4 protein in atrial muscle before and after perfusion. Conclusion: the desensitization of acetylcholine on human atrial muscle may be related to the desensitization of IK,Ach channel current in human atrial muscle, but not to the expression of IK,Ach channel protein.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R331
本文编号：2289140
[Abstract]:Effect of acetylcholine (ACh) on electrophysiological characteristics of isolated human atrial myography objective: to study the effect of vagus neurotransmitter acetylcholine (ACh) on the electrophysiological characteristics of human atrial myograph in vitro Methods: twenty patients with congenital heart disease were selected. The tissue of the right atrial appendage was removed during the operation, and the standard glass microelectrode technique and tension transducer were used. The time course (APD90) of resting membrane potential (RMP),) of right atrial cells before and after perfusion of acetylcholine (ACh) to 90% repolarization and the changes of spontaneous action potential and contractility were measured. Results: acetylcholine significantly increased the resting potential of human atrial muscle (RMP) (54.7 卤2.19mv vs 61.4 卤1.8mv), shortened APD90 (406.3 卤14.61ms vs 316.2 卤15.40ms), and significantly shortened APD90 (P 0.05). Acetylcholine could significantly inhibit the spontaneous action potential and contractility of human atrial muscle (60.5 卤3.2 times / min vs 0 times / min, P 0.05). And the above effects gradually weaken with time. Conclusion: acetylcholine can significantly increase the resting potential of human atrial muscle and shorten the duration of action potential, inhibit the spontaneous action potential and contractility of human atrial muscle, and the above effects have desensitization phenomenon. Desensitization of human atrial muscle by diacetylcholine (ACh) objective: to study the desensitization mechanism of vagus neurotransmitter acetylcholine (ACh) on human atrial muscle in vitro Methods: twenty patients with congenital heart disease were selected. The right atrial appendage tissue was removed. The changes of IK,Ach channel current density in human atrial myocytes and the expression of Kir3.4 protein in human atrial myocytes before and after perfusion were recorded by whole-cell patch clamp technique and Western Blotting Western blotting technique. Results: acetylcholine could activate the IK,Ach channel in human atrial myocytes, and this current had obvious desensitization phenomenon. There was no significant difference in the expression of Kir3.4 protein in atrial muscle before and after perfusion. Conclusion: the desensitization of acetylcholine on human atrial muscle may be related to the desensitization of IK,Ach channel current in human atrial muscle, but not to the expression of IK,Ach channel protein.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R331
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1 张昌伟;乙酰胆碱（ACh）对离体人心房肌电生理特性的影响及其脱敏机制研究[D];华中科技大学;2008年
，本文编号：2289140
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