铝佐剂吸附CpG ODN增强重组乙肝疫苗免疫效应

发布时间：2018-10-25 07:38

【摘要】：目的探索新佐剂CpG ODN在重组乙肝疫苗中的免疫效应及吸附状态。方法 CpG ODN佐剂与重组乙肝疫苗混合免疫小鼠,收集小鼠血清检测。通过免疫小鼠后的体液和细胞免疫应答水平来评价双佐剂乙肝疫苗对重组乙肝疫苗的增强作用。CpG ODN佐剂与乙肝疫苗在不同温度条件下混合吸附,计算在不同吸附时间下CpG ODN佐剂在乙肝疫苗中的吸附率。结果CpG ODN佐剂可以提高重组乙肝疫苗在体液及细胞中的免疫效果。CpG ODN佐剂在室温条件下1 h即可在乙肝疫苗中达到吸附平衡,于2~8℃放置5 d的条件下其吸附率约在50%左右。结论 CpG ODN能够增加乙肝疫苗的免疫应答,其免疫效果可提高5倍以上。
[Abstract]:Objective to investigate the immune effect and adsorption state of new adjuvant CpG ODN in recombinant hepatitis B vaccine. Methods mice were immunized with CpG ODN adjuvant and recombinant hepatitis B vaccine. The effects of double adjuvant hepatitis B vaccine on the enhancement of recombinant hepatitis B vaccine were evaluated by the humoral and cellular immune response levels of mice immunized. The. CpG ODN adjuvant and hepatitis B vaccine were mixed and adsorbed at different temperatures. The adsorption rate of CpG ODN adjuvant in hepatitis B vaccine was calculated at different adsorption time. Results CpG ODN adjuvant could improve the immune effect of recombinant hepatitis B vaccine in body fluid and cells. The adsorption equilibrium of. CpG ODN adjuvant in hepatitis B vaccine could be reached at room temperature for 1 h, and the adsorption rate of. CpG ODN adjuvant was about 50% at 2 ~ 8 鈩，

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