腺嘌呤与氢化可的松所致大鼠肾阳虚证模型比较研究

发布时间：2018-11-04 13:57

【摘要】：目的:比较腺嘌呤与氢化可的松所致大鼠肾阳虚证模型的特点。方法:将50只雄性SD大鼠随机分为对照组(10只)、腺嘌呤组(20只)、氢化可的松组(20只),模型组分别给予腺嘌呤混悬液灌胃和肌内注射氢化可的松,造模21d后,将各模型组大鼠均分为模型组和给药组,给药组给予肾气丸混悬液灌胃21d。处死大鼠后,检测下丘脑促肾上腺激素释放激素(CRH),血清肾上腺皮质激素(ACTH)、肾上腺皮质酮(CORT)、促甲状腺素(TSH)、三碘甲状腺原氨酸(T3)、甲状腺素(T4),尿17-羟皮质类固醇(17-OHCS)及肾髓质水通道蛋白(AQP)1、AQP2含量,取肾组织进行HE染色。结果:与对照组比较,模型组大鼠下丘脑CRH、血清ACTH、CORT、T3、T4、TSH及尿17-OHCS含量显著下降(P0.01);腺嘌呤组大鼠肾髓质AQP1、AQP2含量显著下降,肾小球扩张,肾小管内有针状褐色结晶沉积,氢化可的松组这些指标无显著变化;肾气丸干预后,各给药组大鼠下丘脑CRH、血清ACTH、CORT、T3、T4、TSH及尿17-OHCS含量显著升高(P0.01,P0.05),腺嘌呤给药组大鼠肾髓质AQP1、AQP2含量显著升高,肾损伤明显改善。结论:腺嘌呤诱导的大鼠肾阳虚模型水液代谢紊乱明显,肾损伤明显氢化可的松诱导的大鼠肾阳虚模型表现为HPA轴受抑制。
[Abstract]:Objective: to compare the characteristics of kidney yang deficiency model induced by adenine and hydrocortisone in rats. Methods: fifty male SD rats were randomly divided into control group (n = 10), adenine group (n = 20) and hydrocortisone group (n = 20). The rats in each model group were divided into two groups: model group and administration group. The rats in the treatment group were given Shenqi pill suspension for 21 days. The hypothalamic adrenocorticotropin releasing hormone (CRH),) serum (ACTH), adrenocorticosterone (CORT), thyrotropin (TSH), triiodothyronine (T3) and thyroxine (T4) were detected after the rats were killed. Urine 17-hydroxycorticosteroid (17-OHCS) and renal medullary aquaporin (AQP) 1) were stained with HE. Results: compared with the control group, the content of serum ACTH,CORT,T3,T4,TSH and urinary 17-OHCS in the model group decreased significantly (P0.01). The content of AQP1,AQP2 in renal medulla decreased significantly in adenine group, glomerular dilatation, and acicular brown crystal deposition in renal tubules, but there was no significant change in hydrocortisone group. After intervention by Shenqi Pill, the contents of serum ACTH,CORT,T3,T4,TSH and urinary 17-OHCS in hypothalamus of rats in each group were significantly increased (P 0.05), and the content of AQP1,AQP2 in renal medulla of rats treated with adenine was significantly higher than that in control group. Renal injury was significantly improved. Conclusion: adenine induced rat model of kidney yang deficiency has obvious water and fluid metabolism disorder, and the model of kidney injury induced by hydrocortisone shows that the HPA axis is inhibited.
【作者单位】：浙江中医药大学;
【基金】：国家自然科学基金项目(No.81173143,No.81373507) 高等学校博士学科点专项科研基金资助项目(No.20103322110003)~~
【分类号】：R-332;R228

【参考文献】