缺血缺氧状态下溶血磷脂酸对豚鼠心肌细胞作用的实验研究

发布时间：2018-11-06 15:26

【摘要】： 目的:通过观察外源性溶血磷脂酸(LPA)对缺血缺氧状态下豚鼠心室肌细胞动作电位及肌纤维膜上ATP敏感钾通道电流,豚鼠心室肌细胞线粒体膜电位的影响,探讨LPA对缺血缺氧心肌细胞的作用及其机理。方法:应用全细胞膜片钳技术,观察LPA对缺血缺氧状态下豚鼠心室肌细胞动作电位的影响;观察LPA对缺血缺氧状态下豚鼠心室肌细胞肌纤维膜上ATP敏感钾通道电流的影响;采用细胞培养和流式细胞技术,观察LPA对缺血缺氧豚鼠心室肌细胞线粒体膜电位的影响。结果:外源性LPA可以缩短缺血缺氧豚鼠心室肌细胞动作电位时程,增强缺血缺氧豚鼠心室肌细胞肌纤维膜上ATP敏感钾通道的电流幅度,并且升高缺血缺氧的豚鼠心室肌细胞线粒体膜电位。结论:外源性LPA通过增加缺血缺氧豚鼠心室肌细胞肌纤维膜上ATP敏感钾通道电流,缩短动作电位时程,减少钙离子内流,降低细胞内游离钙浓度,保护缺血缺氧心肌细胞;同时外源性LPA可以升高缺血缺氧豚鼠心室肌细胞线粒体膜电位,防止线粒体内钙超载,保护缺血缺氧心肌细胞。创新点: 1.外源性LPA缩短缺血缺氧豚鼠心室肌细胞动作电位时程。 2.外源性LPA增强缺血缺氧豚鼠心室肌细胞肌纤维膜上ATP敏感钾通道电流幅度。 3.外源性LPA提高缺血缺氧豚鼠心室肌细胞线粒体膜电位。
[Abstract]:Aim: to observe the effects of exogenous lysophosphatidic acid (LPA) on action potential of guinea pig ventricular myocytes, ATP sensitive potassium channel current on myofilm and mitochondrial membrane potential of guinea pig ventricular myocytes under ischemia and hypoxia. To investigate the effect and mechanism of LPA on ischemic and hypoxic cardiomyocytes. Methods: whole cell patch clamp technique was used to observe the effect of LPA on the action potential of guinea pig ventricular myocytes under ischemia and hypoxia, and to observe the effect of LPA on the current of ATP sensitive potassium channel in the myocyte membrane of guinea pig ventricular myocytes under the condition of ischemia and hypoxia. Cell culture and flow cytometry were used to observe the effect of LPA on mitochondrial membrane potential in guinea pig ventricular myocytes after ischemia and hypoxia. Results: exogenous LPA could shorten the duration of action potential of guinea pig ventricular myocytes after ischemia and hypoxia, and enhance the current amplitude of ATP sensitive potassium channel on the membrane of ventricular myocytes of ischemic hypoxia guinea pig ventricular myocytes. The mitochondrial membrane potential of guinea pig ventricular myocytes with ischemia and hypoxia was increased. Conclusion: exogenous LPA can shorten the duration of action potential, decrease the influx of calcium ion, decrease the concentration of intracellular free calcium and protect myocardial cells from ischemia and hypoxia by increasing the current of ATP sensitive potassium channel on the myocyte membrane of guinea pig ventricular myocytes after ischemia and hypoxia. At the same time, exogenous LPA could increase mitochondrial membrane potential of ventricular myocytes from ischemia and hypoxia, prevent calcium overload in mitochondria and protect myocardial cells from ischemia and hypoxia. Innovation: 1. Exogenous LPA shortens the duration of action potential in guinea pig ventricular myocytes after ischemia and hypoxia. 2. Exogenous LPA enhanced the current amplitude of ATP sensitive potassium channel in myocyte membrane of guinea pig ventricular myocytes after ischemia and hypoxia. 3. Exogenous LPA enhances mitochondrial membrane potential in guinea pig ventricular myocytes after ischemia and hypoxia.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R363

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