身痛逐瘀汤对腰椎退变模型大鼠纤维环细胞p38MAPK信号通路的影响

发布时间：2018-11-11 19:31

【摘要】：目的:观察身痛逐瘀汤对腰椎退变模型大鼠纤维环细胞p38MAPK信号通路的影响。方法:24只雄性SD大鼠按随机数字表法分为身痛逐瘀汤组、模型组、假手术组,每组8只。身痛逐瘀汤组、模型组采用纤维环穿刺法建立大鼠腰椎退变模型,造模1个月后行中药灌胃治疗,身痛逐瘀汤组予身痛逐瘀汤灌胃,模型组及假手术组予等量生理盐水灌胃,每日1次,连续4周。治疗结束后处死大鼠取出椎间盘,采用免疫组化法观察纤维环细胞p-p38、p38和NF-κB蛋白表达,Western Blot法测定p-p38、p38和NF-κB蛋白含量。结果:身痛逐瘀汤组p-p38、NF-κB蛋白表达较模型组明显减少(P0.05),模型组较假手术组明显增加(P0.01),假手术组p-p38、NF-κB蛋白少或无表达;3组间p38蛋白表达差异无统计学差异(P0.05)。结论:身痛逐瘀汤可延缓椎间盘退变,其机制可能与下调p-p38和NF-κB蛋白表达,抑制p38MAPK信号通路激活有关。
[Abstract]:Aim: to observe the effect of Shentong Zhuyu decoction on p38MAPK signal pathway of fibrocytic cells in lumbar degenerative model rats. Methods: Twenty-four male SD rats were randomly divided into three groups: Shentong Zhuyu decoction group, model group, sham-operation group, 8 rats in each group. The model group was treated with traditional Chinese medicine one month later, the model group was treated with Shentong Zhuyu decoction, and the model group and sham-operation group were given the same amount of normal saline. Once a day for 4 weeks. After the treatment, the intervertebral discs were removed and the expression of p-p38 and NF- 魏 B protein in fibroblasts was observed by immunohistochemical method. The protein levels of p-p38 and NF- 魏 B were measured by, Western Blot method. Results: compared with the model group, the expression of p-p38 + NF- 魏 B protein in the Shentong Zhuyu decoction group was significantly lower than that in the model group (P0.05), and the expression of p-p38 + NF- 魏 B protein in the model group was significantly higher than that in the sham-operation group (P0.01). There was no significant difference in the expression of p38 protein among the three groups (P0.05). Conclusion: Shentong Zhuyu decoction can delay the degeneration of intervertebral disc, and its mechanism may be related to the down-regulation of p-p38 and NF- 魏 B protein expression and the inhibition of activation of p38MAPK signaling pathway.
【作者单位】：福建中医药大学康复医学院;福建生物工程职业技术学院;
【基金】：福建省卫生厅医学创新项目(2014-CXB-20)
【分类号】：R285.5;R-332

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