经前期综合征肝气郁证模型大鼠不同脑区ERα、ERβ的分布与表达
发布时间：2018-11-18 17:04
【摘要】：目的：检测经前期综合征(premenstrual syndrome,PMS)肝气郁证模型大鼠海马、下丘脑相关区域内ERα和ERβ蛋白分布、表达的变化,初步探讨PMS肝气郁证发病的中枢机制,并研究中药经前舒颗粒对该病证的干预作用。 方法：以旷场实验、宏观行为药理学、阴道涂片法筛选合适大鼠进入实验,采用束缚造模法复制PMS肝气郁证大鼠模型,以旷场实验结合宏观行为药理学评价模型,采用免疫组织化学技术ABC法检测正常对照组、PMS肝气郁模型组、模型给药组大鼠海马CA1、CA3、下丘脑腹内侧区(VMH)中ERα和ERβ的定位分布与积分光密度值(IOD),计算IOD(ERβ)/IOD(ERα). 结果：与正常组大鼠相比,模型组大鼠体重显著减轻(P0.01),旷场实验水平得分、垂直得分、旷场实验总分均显著减少(P0.01),大鼠表现出精神萎靡,眼神呆滞,对外界刺激不敏感的状态；而造模并给予经前舒颗粒的大鼠较模型组大鼠体重增长显著(P0.05),旷场实验三项得分均显著增加(P0.05或P0.01)。免疫组化结果显示,三组大鼠海马、下丘脑中ERα和ERβ的定位分布无显著性差异。与正常组大鼠相比,模型组大鼠海马CA1、CA3区锥体细胞出现形态变化,海马CA1、CA3、下丘脑VMH区ERα和ERβIOD显著升高(P0.05或P0.01),IOD(ERβ)/IOD(ERα)没有显著性差异。造模并给药的大鼠海马结构无异常；与模型组相比,海马CA1、CA3、下丘脑VMH区ERα和ERβIOD显著降低(P0.01),下丘脑VMH区IOD(ERβ)/IOD(ERα)显著下降(P0.05)。 结论：采用束缚造模法可以成功制备PMS肝气郁证大鼠模型,旷场实验结合宏观行为学可有效评价模型。大鼠海马CA1、CA3、下丘脑VMH区ERα、ERβ蛋白表达升高可能与PMS肝气郁证关系密切,可能是PMS肝气郁证发病的中枢机制之一；中药经前舒颗粒可以有效纠正模型大鼠上述脑区ERα、ERβ蛋白表达异常升高的状态,同时下调下丘脑VMH区ERβ/ERα,可能是该药治疗PMS肝气郁证的机制之一。
[Abstract]:Objective: to investigate the distribution and expression of ER 伪 and ER 尾 protein in hippocampus and hypothalamus of rats with liver qi depression syndrome (premenstrual syndrome,PMS), and to explore the central mechanism of PMS liver qi depression syndrome. And to study the intervention effect of Jingqianshu granule on the syndrome of the disease. Methods: open field experiment, macroscopic behavior pharmacology and vaginal smear were used to screen suitable rats to enter the experiment. The rat model of PMS liver qi depression was established by binding method. The open field experiment combined with macroscopic behavior pharmacological evaluation model was used. Localization and distribution of ER 伪 and ER 尾 in (VMH) of hypothalamic ventromedial area of hippocampus CA1,CA3, in rats of normal control group, PMS liver qi depression model group and model group by immunohistochemical technique ABC method and integrated optical density (IOD),) IOD (ER 尾 / IOD (ER 伪). Results: compared with the normal group, the rats in the model group were significantly reduced in body weight (P0.01), open field test level score, vertical score and total open field test score (P0.01). The state of being insensitive to external stimuli; Compared with the model group, the weight of the rats in the model group increased significantly (P0.05), and the scores of the three items in the open field test were significantly increased (P0.05 or P0.01). Immunohistochemical results showed that the localization of ER 伪 and ER 尾 in hippocampus and hypothalamus of the three groups had no significant difference. Compared with the normal group, the hippocampal CA1,CA3 pyramidal cells in the model group showed morphological changes, but ER 伪 and ER 尾 IOD in the VMH area of the hypothalamus of hippocampus CA1,CA3, were significantly increased (P0.05 or P0.01), IOD (ER 尾 / IOD (ER 伪). Compared with the model group, the ER 伪 and ER 尾 IOD in the hypothalamic VMH area of hippocampus CA1,CA3, decreased significantly (P0.01), and IOD (ER 尾 / IOD (ER 伪 in the VMH area of the hypothalamus decreased significantly (P0.05). Conclusion: the rat model of PMS liver qi depression can be successfully established by using the method of restraint. Open field experiment combined with macroscopical behavior can effectively evaluate the model. The increased expression of ER 伪 and ER 尾 in the hypothalamus of CA1,CA3, may be closely related to the stagnation of liver qi of PMS, and may be one of the central mechanisms of PMS syndrome of liver qi depression. Jingqianshu granule can effectively correct the abnormal increase of ER 伪 and ER 尾 protein expression in the above-mentioned brain regions of the model rats, and down-regulate ER 尾 / ER 伪 in the hypothalamic VMH area, which may be one of the mechanisms of the treatment of PMS liver qi stagnation syndrome.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332
本文编号：2340609
[Abstract]:Objective: to investigate the distribution and expression of ER 伪 and ER 尾 protein in hippocampus and hypothalamus of rats with liver qi depression syndrome (premenstrual syndrome,PMS), and to explore the central mechanism of PMS liver qi depression syndrome. And to study the intervention effect of Jingqianshu granule on the syndrome of the disease. Methods: open field experiment, macroscopic behavior pharmacology and vaginal smear were used to screen suitable rats to enter the experiment. The rat model of PMS liver qi depression was established by binding method. The open field experiment combined with macroscopic behavior pharmacological evaluation model was used. Localization and distribution of ER 伪 and ER 尾 in (VMH) of hypothalamic ventromedial area of hippocampus CA1,CA3, in rats of normal control group, PMS liver qi depression model group and model group by immunohistochemical technique ABC method and integrated optical density (IOD),) IOD (ER 尾 / IOD (ER 伪). Results: compared with the normal group, the rats in the model group were significantly reduced in body weight (P0.01), open field test level score, vertical score and total open field test score (P0.01). The state of being insensitive to external stimuli; Compared with the model group, the weight of the rats in the model group increased significantly (P0.05), and the scores of the three items in the open field test were significantly increased (P0.05 or P0.01). Immunohistochemical results showed that the localization of ER 伪 and ER 尾 in hippocampus and hypothalamus of the three groups had no significant difference. Compared with the normal group, the hippocampal CA1,CA3 pyramidal cells in the model group showed morphological changes, but ER 伪 and ER 尾 IOD in the VMH area of the hypothalamus of hippocampus CA1,CA3, were significantly increased (P0.05 or P0.01), IOD (ER 尾 / IOD (ER 伪). Compared with the model group, the ER 伪 and ER 尾 IOD in the hypothalamic VMH area of hippocampus CA1,CA3, decreased significantly (P0.01), and IOD (ER 尾 / IOD (ER 伪 in the VMH area of the hypothalamus decreased significantly (P0.05). Conclusion: the rat model of PMS liver qi depression can be successfully established by using the method of restraint. Open field experiment combined with macroscopical behavior can effectively evaluate the model. The increased expression of ER 伪 and ER 尾 in the hypothalamus of CA1,CA3, may be closely related to the stagnation of liver qi of PMS, and may be one of the central mechanisms of PMS syndrome of liver qi depression. Jingqianshu granule can effectively correct the abnormal increase of ER 伪 and ER 尾 protein expression in the above-mentioned brain regions of the model rats, and down-regulate ER 尾 / ER 伪 in the hypothalamic VMH area, which may be one of the mechanisms of the treatment of PMS liver qi stagnation syndrome.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332
【参考文献】
相关期刊论文 前10条
1 乔明琦,张惠云,陈雨振,韩秀珍,姚泽贤,刘长华,宋承木;肝郁证动物模型研究的理论思考[J];中国医药学报;1997年05期
2 张艳美;慢性应激、大脑损害与抑郁症[J];国外医学.精神病学分册;2001年02期
3 刘晓伟,张红梅,曲宏达,殷平善;“怒伤气”大鼠行为观察与检测[J];江苏中医药;2005年03期
4 张吉强,蔡文琴;雌激素 β受体免疫阳性神经元在成年雌性大鼠脑内的分布[J];解剖学报;2002年02期
5 张庆长,郭晓红,沈伟,李云生,田德润;雌性大鼠下丘脑腹内侧核神经元雌激素受体研究[J];天津医科大学学报;1999年01期
6 鲁明源;情志刺激内伤发病的机理探讨[J];山东中医药大学学报;1995年01期
7 屠霞芬;大运动量游泳对小白鼠动情周期的影响[J];中国临床康复;2004年36期
8 陈芳萍,李运曼,刘国卿;5-HT_(1A)受体激动剂类抗抑郁药的研究与开发[J];药学进展;2003年04期
9 乔明琦,张珍玉,徐旭杰,韩秀琴,慈玉珍,叶青;经前期综合征证候分布规律的流行病学调查研究[J];中国中医基础医学杂志;1997年03期
10 彭伟锋;汪昕;马昱;;雌激素和克罗米酚对致vN大鼠海马γ-氨基丁酸免疫反应细胞和GABA_A受体α1亚单位表达的影响[J];中华神经科杂志;2005年12期
相关博士学位论文 前1条
1 陈煜辉;中医情志学科建设及情志病证临床防治框架构建探讨[D];广州中医药大学;2005年
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