骨桥蛋白在肝素结合表皮生长因子样生长因子介导的小鼠胚胎对子宫内膜黏附和扩展中的作用研究

发布时间：2018-11-28 19:44

【摘要】：目的:探讨肝素结合表皮生长因子样生长因子(HB-EGF)对小鼠胚胎黏附和扩展中的作用机制。方法:建立小鼠胚胎与小鼠子宫内膜细胞共培养模型,分A、B、C、D、E共5组,分别加入不同浓度的HB-EGF(0μg/L、20μg/L、50μg/L、100μg/L、200μg/L),于倒置显微镜下统计各组胚胎黏附率及扩展率;用免疫组织化学法检测小鼠子宫内膜细胞骨桥蛋白(OPN)的表达及定位,Western blotting法测定各浓度HB-EGF对子宫内膜细胞OPN表达水平的影响,并分析其与HB-EGF的关联。结果:OPN在小鼠子宫内膜细胞高表达,主要分布于胞质中,随HB-EGF浓度升高,小鼠子宫内膜细胞中OPN的表达增强,小鼠胚胎对子宫内膜的黏附和扩展率也逐渐增高。结论:HBEGF可能通过上调小鼠子宫内膜细胞OPN的表达,促进小鼠胚胎对子宫内膜的黏附和扩展。
[Abstract]:Aim: to investigate the mechanism of heparin binding epidermal growth factor like growth factor (HB-EGF) on mouse embryo adhesion and expansion. Methods: a co-culture model of mouse embryo and mouse endometrial cells was established. The mice were divided into 5 groups, and were treated with different concentrations of HB-EGF (0 渭 g / L, 20 渭 g / L, 50 渭 g / L, 100 渭 g / L, 200 渭 g / L), respectively. The rates of embryo adhesion and expansion were calculated under inverted microscope. The expression of osteopontin (OPN) in mouse endometrial cells was detected by immunohistochemical method, and the effect of HB-EGF concentration on OPN expression in endometrial cells was determined by localization, Western blotting method, and its association with HB-EGF was analyzed. Results: the expression of OPN was highly expressed in mouse endometrial cells, mainly distributed in the cytoplasm. With the increase of HB-EGF concentration, the expression of OPN in mouse endometrial cells increased, and the adhesion and expansion rate of mouse embryos to endometrium increased gradually. Conclusion: HBEGF may promote the adhesion and expansion of mouse embryo to endometrium by up-regulating the expression of OPN in mouse endometrial cells.
【作者单位】：广州医科大学附属广东省妇幼保健院生殖健康与不孕不育症科;
【基金】：广东省自然科学基金项目;项目号:S2013010013098
【分类号】：R321

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