亚血红素六肽抗氧化损伤作用的研究
发布时间：2018-12-11 06:18
【摘要】： 本实验室设计并合成的含有组氨酸残基的单体亚血红素六肽(Deuterohaemin-Ala-His-Thr-Val-Glu-Lys, DhHP-6),具有较高的抗坏血酸过氧化物酶活性,活性可达3.9x103U·μmol-1(天然微过氧化物酶MP-11的93%),是一种新的过氧化物酶模拟物,其分子量小,可进入心肌细胞;它能类似人体自然过程,有效地清除体内的过氧化物、超氧离子和自由基。 随着多种血管再通技术在临床的广泛应用,心肌缺血再灌注(IR)损伤已越来越受到广大科研工作者的重视,但其作用机制至今尚未完全清楚。本课题采用培养的HeLa细胞和H9C2细胞建立缺氧再给氧(HR)损伤模型,从细胞水平研究DhHP-6对培养细胞缺氧再给氧损伤的保护作用,并对其作用机制进行初步探讨。通过对培养的HeLa细胞和H9C2细胞的形态观察,细胞存活率的测定,Ca++-ATPase活力的测定,LDH活力的测定,细胞活力的测定,AO/EB双染以及H9C2细胞SOD活力的测定和MDA含量的测定,证明DhHP-6具有减轻细胞缺氧再给氧损伤的作用,其机制与对抗氧自由基、降低细胞内[Ca2+]有关。
[Abstract]:The monomer heme hexapeptide (Deuterohaemin-Ala-His-Thr-Val-Glu-Lys, DhHP-6) with histidine residue was designed and synthesized in our laboratory. It has high activity of ascorbic acid peroxidase (ascorbic acid peroxidase). The activity of 3.9x103U 渭 mol-1 (93% of the natural microperoxidase MP-11) is a new peroxidase mimic, and its molecular weight is small and it can enter cardiomyocytes. It can be similar to the natural process of the human body, effectively scavenging the body of peroxides, superoxide ions and free radicals. With the wide application of various vascular recanalization techniques, myocardial ischemia-reperfusion (IR) injury has been paid more and more attention by researchers, but its mechanism has not been fully understood. In this study, HeLa cells and H9C2 cells were used to establish the model of (HR) injury induced by hypoxia and reoxygenation. The protective effect of DhHP-6 on hypoxia and reoxygenation injury of cultured cells was studied at the cell level, and the mechanism was discussed. The morphology of cultured HeLa cells and H9C2 cells, cell survival rate, Ca ATPase activity, LDH activity, cell viability, AO/EB double staining, SOD activity of H9C2 cells and MDA content were measured. It is proved that DhHP-6 can alleviate the damage of hypoxia and reoxygenation, and its mechanism is related to the inhibition of oxygen free radical and the decrease of intracellular [Ca2].
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R341
本文编号：2372037
[Abstract]:The monomer heme hexapeptide (Deuterohaemin-Ala-His-Thr-Val-Glu-Lys, DhHP-6) with histidine residue was designed and synthesized in our laboratory. It has high activity of ascorbic acid peroxidase (ascorbic acid peroxidase). The activity of 3.9x103U 渭 mol-1 (93% of the natural microperoxidase MP-11) is a new peroxidase mimic, and its molecular weight is small and it can enter cardiomyocytes. It can be similar to the natural process of the human body, effectively scavenging the body of peroxides, superoxide ions and free radicals. With the wide application of various vascular recanalization techniques, myocardial ischemia-reperfusion (IR) injury has been paid more and more attention by researchers, but its mechanism has not been fully understood. In this study, HeLa cells and H9C2 cells were used to establish the model of (HR) injury induced by hypoxia and reoxygenation. The protective effect of DhHP-6 on hypoxia and reoxygenation injury of cultured cells was studied at the cell level, and the mechanism was discussed. The morphology of cultured HeLa cells and H9C2 cells, cell survival rate, Ca ATPase activity, LDH activity, cell viability, AO/EB double staining, SOD activity of H9C2 cells and MDA content were measured. It is proved that DhHP-6 can alleviate the damage of hypoxia and reoxygenation, and its mechanism is related to the inhibition of oxygen free radical and the decrease of intracellular [Ca2].
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R341
【引证文献】
相关硕士学位论文 前3条
1 朱媛媛;亚铁血红素的酶法制备及功能特性研究[D];吉林大学;2011年
2 张超;次血红素六肽抗纳米金介导的线虫热损伤作用研究[D];吉林大学;2010年
3 冯建宇;钙通道阻断剂对低钾状态下大鼠缺血—再灌注心功能恢复的作用观察[D];第四军医大学;2012年
，本文编号：2372037
本文链接：https://www.wllwen.com/yixuelunwen/shiyanyixue/2372037.html
最近更新
教材专著
