在皂甙和丙烯酰胺诱导下海兔脑神经节及神经连索的差异蛋白质组研究
发布时间：2018-12-11 16:35
【摘要】： 海兔(Aplysia)归属于腹足纲软体动物,它的中枢神经系统(central nervoussystem,CNS)是开展分子神经生物学研究的经典模式之一。研究海兔CNS功能蛋白质和多肽结构与功能有助于科学地揭示人及高等动物神经系统的活动规律和调控机制,例如:记忆形成、神经性生理调控和神经系统疾病起因等。三七(Panax notoginseng,PN)是中国传统中草药之一,其主要成分为皂甙,具有促智、延缓衰老等保护神经系统功能。 采用薄层层析(TLC)、高效液相色谱(HPLC)和基质辅助激光解吸附飞行时间质谱(MALDI-TOF-MS)分析三七提取液(Panax notoginseng extract,PNE)组成,指出三七的主要成分为人参皂甙Rb1、Rg1、Re和三七皂甙R1,占总皂甙80%以上。 选用蓝斑背纲海兔(Notarcus Ieachii cirrosus Stimpson,NLCS)的CNS为实验材料,采用蛋白质组技术,初步鉴定了NLCS受PNE诱导后,其神经连索表达差异蛋白有肌动蛋白、3-羟酯酰辅酶A脱氢酶、ABC转运子、甲基转移酶等。经R1诱导后,其神经连索表达的差异蛋白是凋亡抑制蛋白、26S蛋白酶体、酰基辅酶A脱氢酶和甲基转移酶等。为了获得更详细的信息,对这些差异蛋白进行了亚细胞定位,通过比对分析后,作者认为这些差异蛋白可能与学习记忆,神经系统疾病有关。阐明PNE和R1的作用机制起着重要的作用,其结果可为今后开展神经疾病预防和治疗提供科学依据和新颖途径。 丙烯酰胺(Acrylamide,ACR)是一种常见的神经毒剂,它直接损伤神经系统。三七皂甙组成能起到减缓神经损伤的作用。选用杂斑海兔(Aplysia juliana,AJ)的CNS为实验材料,筛选出AJ经ACR毒害后,PNE对AJ大脑神经节产生恢复(拮抗)过程中的差异蛋白质。指出AJ的大脑神经节经ACR毒害后,产生了24个差异蛋白,但经PNE恢复处理后,产生了拮抗效应,消除了多数差异蛋白质的表达,使其表达量接近对照组,PNE+ACR和ACR+PNE的效果并不完全一样。经过数据库检索,发现有3个蛋白质已经报道与神经系统损伤有关,分别是热休克蛋白20、磷酸丙糖异构酶和短链脱氢酶。此研究有助于阐明ACR在脑神经节代谢过程中所产生的毒性机理,以及探讨PNE在保护ACR毒性效应的方式,对实践应用具有指导意义。
[Abstract]:(Aplysia) belongs to Gastropoda mollusc and its central nervous system (central nervoussystem,CNS) is one of the classical models of molecular neurobiology. The study of the structure and function of CNS functional proteins and polypeptides in sea rabbits is helpful to reveal the activity and regulation mechanism of nervous system in human and higher animals, such as memory formation, neurophysiological regulation and the causes of nervous system diseases. Panax notoginseng (Panax notoginseng,PN) is one of the traditional Chinese herbal medicines, its main component is saponin, which has the function of promoting intelligence, delaying senescence and protecting nervous system. (TLC), high performance liquid chromatography (HPLC) and matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF-MS) were used to analyze the composition of Panax notoginseng extract (Panax notoginseng extract,PNE. It was pointed out that the main component of Panax notoginseng was ginsenoside Rb1,Rg1,. Re and Panax notoginsenoside R 1 accounted for more than 80% of the total saponins. The CNS of (Notarcus Ieachii cirrosus Stimpson,NLCS was selected as the experimental material, and the differential expression of actin and 3-hydroxyester coenzyme A dehydrogenase in the neuronal connectors of NLCS induced by PNE were preliminarily identified by proteome technique. ABC transporter, methyltransferase, etc. After induction by R1, the differentially expressed proteins were apoptosis inhibitor protein, 26s proteasome, acyl coenzyme A dehydrogenase and methyltransferase. In order to obtain more detailed information, subcellular localization of these differential proteins was carried out. After comparative analysis, the authors concluded that these differential proteins might be related to learning, memory and nervous system diseases. The mechanism of PNE and R1 plays an important role, and the results may provide scientific basis and novel approaches for the prevention and treatment of neurological diseases in the future. Acrylamide (Acrylamide,ACR) is a common nerve agent that directly damages the nervous system. The composition of Panax notoginseng saponins can attenuate the nerve injury. The CNS of Aplysia juliana,AJ rabbits was used as the experimental material to screen the differential proteins in the recovery (antagonism) of AJ brain ganglion induced by PNE after AJ was poisoned by ACR. It was pointed out that after being poisoned by ACR, 24 differentially expressed proteins were produced in AJ's cerebral ganglion, but after PNE was restored, antagonistic effect was produced, and the expression of most differentially expressed proteins was eliminated, and the expression of most differentially expressed proteins was close to that of the control group. The effects of PNE ACR and ACR PNE are not exactly the same. By searching the database, we found that three proteins have been reported to be related to nervous system injury, namely heat shock protein 20, propanoisomerase phosphate and short chain dehydrogenase. This study is helpful to clarify the toxic mechanism of ACR in the metabolism of cerebral ganglion, and to explore the way of protecting ACR toxicity by PNE, which is of guiding significance for practical application.
【学位授予单位】：厦门大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R285.5;R363
本文编号：2372873
[Abstract]:(Aplysia) belongs to Gastropoda mollusc and its central nervous system (central nervoussystem,CNS) is one of the classical models of molecular neurobiology. The study of the structure and function of CNS functional proteins and polypeptides in sea rabbits is helpful to reveal the activity and regulation mechanism of nervous system in human and higher animals, such as memory formation, neurophysiological regulation and the causes of nervous system diseases. Panax notoginseng (Panax notoginseng,PN) is one of the traditional Chinese herbal medicines, its main component is saponin, which has the function of promoting intelligence, delaying senescence and protecting nervous system. (TLC), high performance liquid chromatography (HPLC) and matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF-MS) were used to analyze the composition of Panax notoginseng extract (Panax notoginseng extract,PNE. It was pointed out that the main component of Panax notoginseng was ginsenoside Rb1,Rg1,. Re and Panax notoginsenoside R 1 accounted for more than 80% of the total saponins. The CNS of (Notarcus Ieachii cirrosus Stimpson,NLCS was selected as the experimental material, and the differential expression of actin and 3-hydroxyester coenzyme A dehydrogenase in the neuronal connectors of NLCS induced by PNE were preliminarily identified by proteome technique. ABC transporter, methyltransferase, etc. After induction by R1, the differentially expressed proteins were apoptosis inhibitor protein, 26s proteasome, acyl coenzyme A dehydrogenase and methyltransferase. In order to obtain more detailed information, subcellular localization of these differential proteins was carried out. After comparative analysis, the authors concluded that these differential proteins might be related to learning, memory and nervous system diseases. The mechanism of PNE and R1 plays an important role, and the results may provide scientific basis and novel approaches for the prevention and treatment of neurological diseases in the future. Acrylamide (Acrylamide,ACR) is a common nerve agent that directly damages the nervous system. The composition of Panax notoginseng saponins can attenuate the nerve injury. The CNS of Aplysia juliana,AJ rabbits was used as the experimental material to screen the differential proteins in the recovery (antagonism) of AJ brain ganglion induced by PNE after AJ was poisoned by ACR. It was pointed out that after being poisoned by ACR, 24 differentially expressed proteins were produced in AJ's cerebral ganglion, but after PNE was restored, antagonistic effect was produced, and the expression of most differentially expressed proteins was eliminated, and the expression of most differentially expressed proteins was close to that of the control group. The effects of PNE ACR and ACR PNE are not exactly the same. By searching the database, we found that three proteins have been reported to be related to nervous system injury, namely heat shock protein 20, propanoisomerase phosphate and short chain dehydrogenase. This study is helpful to clarify the toxic mechanism of ACR in the metabolism of cerebral ganglion, and to explore the way of protecting ACR toxicity by PNE, which is of guiding significance for practical application.
【学位授予单位】：厦门大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R285.5;R363
【引证文献】
相关博士学位论文 前1条
1 应剑;药物活性评价、代谢分析和晶型技术在药物发现中的应用研究[D];北京协和医学院;2011年
相关硕士学位论文 前1条
1 薛晓昀;大豆根瘤菌与促生菌复合系筛选及机理研究[D];中国农业科学院;2011年
，本文编号：2372873
本文链接：https://www.wllwen.com/yixuelunwen/shiyanyixue/2372873.html
最近更新
教材专著
