改良式液氮冷冻法制备犬股骨头坏死动物模型的研究
发布时间：2018-12-16 23:28
【摘要】： 研究背景:股骨头缺血坏死的治疗是骨科临床难题之一,目前尚未发现一种可终止或者逆转已发生股骨头坏死病理过程,从而阻止股骨头进一步塌陷的有效方法。虽然我们已知在股骨头坏死区的修复与重建过程中,坏死骨的吸收导致股骨头强度的下降是塌陷的主要原因,减少或终止坏死骨的吸收可延迟或避免坏死股骨头塌陷的发生。但我们对其发病机理和何种方法能有效治疗股骨头缺血性坏死尚不十分清楚,原因之一可归咎于缺乏一个能真正反映股骨头坏死的病理过程的动物模型。 目的:通过改良式液氮冷冻法造成犬股骨头缺血坏死,探讨股骨头缺血坏死的动物模型制备,建立能模拟人股骨头坏死(osteonecrosis of femoral head ,ONFH)病理生理过程的实验动物模型。 方法:16只犬随机分成4组,采用改良式液氮冷冻法即刻冷冻单侧股骨头,普通饲料饲养2、4、8、16周分别处死4组动物,分别观察X线摄片、大体形态、光镜下形态变化。 结果:改良式液氮冷冻法冷冻犬股骨头X线、大体及组织学形态不同阶段均出现明显变化。 结论:改良式液氮冷冻法为建立股骨头坏死动物模型提供了一种简便、易行的方法,值得进一步深入研究。
[Abstract]:Background: the treatment of avascular necrosis of femoral head is one of the clinical problems in orthopedic department. At present, there is no effective method to stop or reverse the pathological process of femoral head necrosis and prevent the further collapse of femoral head. Although it is known that the reduction of the strength of the femoral head due to the absorption of the necrotic bone is the main cause of the collapse during the repair and reconstruction of the necrotic area of the femoral head, the reduction or termination of the absorption of the necrotic bone can delay or avoid the collapse of the necrotic femoral head. However, the pathogenesis and the effective treatment of avascular necrosis of femoral head are not well understood. One of the reasons can be attributed to the lack of an animal model which can truly reflect the pathological process of avascular necrosis of femoral head. Objective: to establish an experimental animal model of avascular necrosis of femoral head (ANFH) in dogs by using modified liquid nitrogen freezing method to establish an animal model of avascular necrosis of femoral head (ANFH), which can simulate the pathophysiological process of human avascular necrosis of femoral head (osteonecrosis of femoral head, ONFH). Methods: sixteen dogs were randomly divided into 4 groups, and the animals were killed for 16 weeks by using modified liquid nitrogen freezing method to freeze the unilateral femoral head immediately. The animals were killed for 16 weeks. The X-ray films, the gross morphology and the morphological changes under light microscope were observed. Results: there were obvious changes in X-ray, gross and histological morphology of femoral head frozen by modified liquid nitrogen freezing method. Conclusion: the modified liquid nitrogen freezing method provides a simple and easy method for the establishment of animal model of femoral head necrosis.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R681.8;R-332
本文编号：2383233
[Abstract]:Background: the treatment of avascular necrosis of femoral head is one of the clinical problems in orthopedic department. At present, there is no effective method to stop or reverse the pathological process of femoral head necrosis and prevent the further collapse of femoral head. Although it is known that the reduction of the strength of the femoral head due to the absorption of the necrotic bone is the main cause of the collapse during the repair and reconstruction of the necrotic area of the femoral head, the reduction or termination of the absorption of the necrotic bone can delay or avoid the collapse of the necrotic femoral head. However, the pathogenesis and the effective treatment of avascular necrosis of femoral head are not well understood. One of the reasons can be attributed to the lack of an animal model which can truly reflect the pathological process of avascular necrosis of femoral head. Objective: to establish an experimental animal model of avascular necrosis of femoral head (ANFH) in dogs by using modified liquid nitrogen freezing method to establish an animal model of avascular necrosis of femoral head (ANFH), which can simulate the pathophysiological process of human avascular necrosis of femoral head (osteonecrosis of femoral head, ONFH). Methods: sixteen dogs were randomly divided into 4 groups, and the animals were killed for 16 weeks by using modified liquid nitrogen freezing method to freeze the unilateral femoral head immediately. The animals were killed for 16 weeks. The X-ray films, the gross morphology and the morphological changes under light microscope were observed. Results: there were obvious changes in X-ray, gross and histological morphology of femoral head frozen by modified liquid nitrogen freezing method. Conclusion: the modified liquid nitrogen freezing method provides a simple and easy method for the establishment of animal model of femoral head necrosis.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R681.8;R-332
【参考文献】
相关期刊论文 前1条
1 王卫明,赵德伟;液氮冷冻制备股骨头缺血性坏死动物模型研究探讨[J];骨与关节损伤杂志;2001年06期
，本文编号：2383233
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