小鼠孤雌胚胎干细胞直接向神经细胞诱导分化的实验研究

发布时间：2018-12-19 14:29

【摘要】：目的探讨小鼠孤雌胚胎干细胞在体外不经过拟胚体(EB)阶段,直接诱导分化为神经细胞的可行性。方法采用阶段诱导的方法,首先由孤雌胚胎干细胞向神经前体细胞定向分化,通过巢蛋白(nestin)免疫荧光细胞化学染色对神经前体细胞进行鉴定。在此基础上,撤除丝裂原,加入5%胎牛血清,观察已分化的神经前体细胞能否进一步分化为神经细胞,并对分化出的细胞进行免疫荧光细胞化学鉴定。结果经选择培养基培养3 d后的孤雌干细胞绝大多数可诱导为神经前体细胞,nestin呈阳性。加入血清进一步诱导,可分化出β-Ⅲ-tubline阳性的神经细胞。结论小鼠孤雌胚胎干细胞在体外不经EB培养阶段,可直接诱导分化为神经细胞,并且前期可得到大量的神经前体细胞。
[Abstract]:Objective to investigate the feasibility of differentiation of mouse parthenogenetic embryonic stem cells into neural cells without embryoid (EB) in vitro. Methods the neural progenitor cells were differentiated from parthenogenetic embryonic stem cells to neural precursor cells by the method of stage induction. The neural progenitor cells were identified by (nestin) immunofluorescence cytochemical staining. On this basis, the mitogen was removed and 5% fetal bovine serum was added to observe whether the differentiated neural precursor cells could be further differentiated into nerve cells, and the differentiated cells were identified by immunofluorescence cytochemistry. Results the majority of parthenogenetic stem cells cultured in selected medium for 3 days could be induced to be neural precursor cells and nestin was positive. The 尾-鈪，

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