肝郁脾虚型肠易激综合征模型大鼠结肠组织差异蛋白质组学研究

发布时间：2019-01-09 18:15

【摘要】：目的建立肝郁脾虚型肠易激综合征(IBS)模型组和对照组结肠组织蛋白质双向电泳图谱,结合质谱技术鉴定并分析差异表达蛋白,对候选表达差异蛋白进行蛋白质功能和相关研究,进一步阐明肝郁脾虚型IBS病理机制。方法采用双向凝胶电泳法分离IBS模型组和正常对照组结肠粘膜差异蛋白,运用MALDI TOF/TOF串联飞行时间质谱仪进行鉴定,运用Gene Ontology和Pathway Studio生物信息学方法将所鉴定的差异蛋白进行功能分类,分析IBS病理机制中关键蛋白。结果首次运用蛋白组学的方法寻找IBS模型组和正常对照组结肠的差异表达蛋白特征谱,结果发现差异倍数在2.5倍以上的差异蛋白有15个,其中2个蛋白表达上调,13个蛋白表达下调。结论热休克蛋白、硫氧还蛋白、氯离子通道蛋白等可能是肝郁脾虚型IBS病理机制中潜在分子标志物。
[Abstract]:Objective to establish a two-dimensional electrophoresis map of colon protein in (IBS) model group and control group of irritable bowel syndrome with liver stagnation and spleen deficiency, and to identify and analyze the differentially expressed proteins by mass spectrometry. Protein function and related studies of candidate differentially expressed proteins were carried out to further elucidate the pathological mechanism of IBS with liver stagnation and spleen deficiency. Methods differential proteins of colon mucosa in IBS model group and normal control group were separated by two dimensional gel electrophoresis and identified by MALDI TOF/TOF series time of flight mass spectrometer. The differential proteins were classified by Gene Ontology and Pathway Studio bioinformatics, and the key proteins in the pathological mechanism of IBS were analyzed. Results the differential expression protein characteristic profiles of IBS model group and normal control group were searched by proteomics for the first time. The results showed that 15 differentially expressed proteins were more than 2.5 times different, 2 of which were up-regulated. 13 proteins were down-regulated. Conclusion Heat shock protein, thioredoxin and chloride channel protein may be potential molecular markers in the pathogenesis of IBS with liver stagnation and spleen deficiency.
【作者单位】：华南肿瘤学国家重点实验室/中山大学附属肿瘤医院药学部;广州中医药大学第一附属医院药学部;
【基金】：国家自然科学基金(No.81073160) 广东省自然科学基金(No.S2012010010539)
【分类号】：R-332;R228

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