一株Ⅰ型单纯疱疹病毒的分离鉴定及增殖特性

发布时间：2019-01-10 19:56

【摘要】：目的对一株Ⅰ型单纯疱疹病毒(HSV-1)进行分离鉴定和增值特性研究。方法从病人口唇疱液样本中分离得到一株病毒,经噬斑纯化、形态鉴定、序列分析,确定该分离株为HSV-1,被命名为HSVIZW6A。用其分别感染Vero细胞、KMB17细胞、A375细胞,观察其细胞病变情况,并绘制生长曲线,分析病毒的生物学特性。结果 HSVI-ZW6A株病毒以MOI=10感染不同细胞,细胞外和细胞内病毒在0~4h,病毒复制缓慢,4~12h病毒增殖进入对数生长期,12~24h病毒增殖缓慢,进入平台期,但24h之后病毒效价测定显示病毒滴度开始呈现下降的趋势,且细胞外病毒最大量高于细胞内,且HSVI-ZW6A株病毒毒力上高于HSVI-strain17病毒。结论通过本实验成功分离和鉴定了一株Ⅰ型HSV病毒,并对其生物学特性进行了分析,为今后的实验奠定了基础。
[Abstract]:Objective to isolate and identify a herpes simplex virus (HSV-1) strain. Methods A virus was isolated from a sample of oral blister fluid from patients. After plaque purification, morphological identification and sequence analysis, it was confirmed that the isolate was named HSV-1, as HSVIZW6A.. Vero cells, KMB17 cells and A375 cells were infected respectively. The cytopathic changes of A375 cells were observed, and the growth curves were plotted to analyze the biological characteristics of the virus. Results the virus of HSVI-ZW6A strain was infected with different cells by MOI=10. The replication of the virus was slow at 0hs and within the cells, the proliferation of the virus at 412h entered the logarithmic growth phase, and the virus proliferated slowly at 1224 h, and entered the plateau phase. However, after 24 hours, the titer of the virus began to decrease, and the maximum amount of the extracellular virus was higher than that of the intracellular virus, and the virulence of the HSVI-ZW6A strain was higher than that of the HSVI-strain17 virus. Conclusion A strain of HSV virus type I was successfully isolated and identified by this experiment, and its biological characteristics were analyzed, which laid a foundation for future experiments.
【作者单位】：中国医学科学院北京协和医学院医学生物学研究所;昆明医科大学第一附属医院;
【基金】：云南省科技计划项目(2013FZ138,2013FA053,2013FB138)
【分类号】：R373.11

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