2005-2014年上海市O139群霍乱弧菌的分子特征和耐药性研究

发布时间：2019-01-15 07:03

【摘要】：目的分析2005-2014年上海市O139群霍乱弧菌的分子特征和耐药性。方法以聚合酶链反应(PCR)方法检测86株O139群霍乱弧菌的霍乱毒素基因(ctx A)、溶血素基因(hly A)、毒素协调菌毛基因(tcp A)、调控蛋白基因(toxR)和编码霍乱毒素基因的CTXφ基因组基因(zot、ace)。采用脉冲场凝胶电泳(PFGE)和多位点序列分型(MLST)方法对O139群霍乱弧菌菌株进行分子分型。采用WHO推荐的改良K-B纸片法,对O139群霍乱弧菌菌株进行11种抗菌药物(头孢曲松、强力霉素、诺氟沙星、环丙沙星、复方新诺明、丁胺卡那霉素、四环素、氯霉素、萘啶酸、氨苄西林、庆大霉素)敏感试验。结果患者来源O139群霍乱弧菌菌株93.2%(41/44)为产毒株,水产来源菌株58.3%(21/36)为产毒株,6株水体来源菌株均为非产毒株。PFGE分析将O139群霍乱弧菌分为35个型别。MLST分析将O139群霍乱弧菌分为9个ST型,其中流行菌型为ST 69型,非产毒株以ST 162型为主。86株O139群霍乱弧菌经药物敏感试验分析显示O139群霍乱弧菌产毒株的耐药情况比非产毒株严重。O139群霍乱弧菌产毒株对强力霉素、复方新诺明、四环素、萘啶酸、氨苄西林、庆大霉素的耐药率高。O139群霍乱弧菌非产毒株对萘啶酸耐药严重。结论上海市患者和水产来源O139群霍乱弧菌构成以产毒株为主。PFGE分型提示水产为霍乱疫情的主要传染源。O139群霍乱弧菌产毒株高度克隆化,流行菌型为ST 69型,耐药情况严重。
[Abstract]:Objective to analyze the molecular characteristics and drug resistance of Vibrio cholerae O139 in Shanghai from 2005 to 2014. Methods the cholera toxin gene (ctx A), hemolysin gene (hly A), toxin coordination gene (tcp A), of 86 strains of Vibrio cholerae O139 was detected by polymerase chain reaction (PCR). Regulating protein gene (toxR) and CTX 蠁 genome gene (zot,ace) encoding cholera toxin gene. The molecular typing of Vibrio cholerae O139 was carried out by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Eleven antibiotics (ceftriaxone, doxycycline, norfloxacin, ciprofloxacin, compound sulfampicin, amikacin, tetracycline, chloramphenicol) were applied to Vibrio cholerae O139 by modified K-B disk method recommended by WHO. Naphthyronic acid, ampicillin, gentamicin) sensitivity test. Results 93.2% (41 / 44) strains of Vibrio cholerae O139 were isolated from patients and 58.3% (21 / 36) from aquatic products. All of the 6 strains were non-virulent strains. PFGE analysis classified Vibrio cholerae O139 into 35 types. MLST analysis classified Vibrio cholerae O139 into 9 ST types, among which the prevalent type was ST 69. The drug resistance of 86 strains of Vibrio cholerae O139 was more serious than that of non-vibrio cholerae strain O139. The strains produced by Vibrio cholerae O139 were resistant to doxycycline, compound sulfamine and tetracycline. Naphthyronic acid, ampicillin and gentamicin have high drug resistance. Vibrio cholerae O139, a non-vibrio cholerae strain, is highly resistant to nalididinic acid. Conclusion Vibrio cholerae O139 strains from Shanghai patients and aquatic products were mainly composed of vibrio cholerae producing strains. PFGE typing indicated that aquatic products were the main source of infection for cholera. Vibrio cholerae O139 strains were highly cloned, ST 69 was prevalent, and the drug resistance was serious.
【作者单位】：上海市疾病预防控制中心;
【基金】：上海市公共卫生重点学科卫生微生物学(No.12GWZX0801) “十二五”国家科技重大专项(No.2013ZX10004216-001-004)~~
【分类号】：R378.3

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