基于纳米金探针的6-巯基嘌呤分析方法研究

发布时间：2019-01-14 15:55

【摘要】：近年来,金纳米粒子因其具有很多宏观粒子所不具备的小尺寸效应、表面效应、光学效应以及特殊的生物亲和效应,已经广泛应用于临床医学、材料学等领域。而且在医学检验领域,金纳米粒子标记技术成为目前第四大标记技术。金纳米粒子所具备的特殊的物理与化学性质,在许多研究领域中表现出了潜在的应用价值。目前,金纳米粒子的分析应用主要是通过化学修饰后作为光谱探针与其它物质结合反应,但是用不经过任何化学改性的金纳米粒子作探针,结合光度法或RRS法测定药物的分析方法报道还很少见。研究的主要内容有：综述了金纳米粒子的制备和表征方法、光谱性质及其在分析化学中的应用和抗癌药物6-巯基嘌呤的性质、作用及分析现状等。利用金纳米粒子作探针,建立了一种分光光度法检测微量抗癌药物6-巯基嘌呤的新方法。6-巯基嘌呤自组装到金纳米粒子上以后,溶液的颜色由亮红色变为深蓝色,吸收光谱发生明显的变化。反应前,金纳米粒子在520 nm处出现了其典型的等离子共振吸收带,而6-巯基嘌呤在可见区无吸收峰；反应后,520 nm吸收峰处的吸光度降低,而在680 nm处出现一较强吸收峰,该吸收峰为结合产物的待征吸收峰。并且,在该波长下吸光度与6-巯基嘌呤的浓度呈良好线性关系,检出限为17μg/L。对浓度为0.25mg/L的6-巯基嘌呤进行了11次平行测定,RSD为2.9%。将该法用于检测人尿样中的6-巯基嘌呤含量,方法简便、灵敏度高、抗干扰性强,检测结果令人满意。以金纳米粒子为探针,建立了一种共振瑞利散射光谱法检测6-巯基嘌呤的新方法。在pH为5.50的B-R缓冲溶液中,金纳米粒子与6-巯基嘌呤自组装成体积更大的结合产物,从而使体系的共振瑞利散射光谱强度急剧增强。在实验中,研究了其共振瑞利散射光谱特征,并且考察了影响反应的一些因素和共存物质的影响。结果表明：在最佳条件下,6-巯基嘌呤浓度在0.017～0.32 mg/L范围内与共振瑞利散射光谱强度成线性关系,方法的灵敏度较高,其检出限(3σ)为8μg/L,对浓度为0.12mg/L的6-巯基嘌呤进行了11次平行测定,RSD为5.1%。考察共存物质对6-MP测定的影响,发现该方法具有较好的选择性。根据所确定的最佳条件,建立了一种共振瑞利散射光谱法检测6-巯基嘌呤的新方法,并将该方法用于人尿样中6-巯基嘌呤含量的测定,获得较好的结果。
[Abstract]:In recent years, gold nanoparticles have been widely used in clinical medicine, materials science and other fields because of their small size effect, surface effect, optical effect and special biological affinity effect that many macroscopical particles do not have. And in the field of medical testing, gold nanoparticles labeling technology has become the fourth largest marking technology. Gold nanoparticles have special physical and chemical properties, which have shown potential application value in many research fields. At present, the analysis of gold nanoparticles is mainly through chemical modification as a spectral probe to react with other substances, but not through any chemical modification of gold nanoparticles as a probe, Analytical methods combined with photometry or RRS are rarely reported. The main contents of this paper are as follows: the preparation and characterization of gold nanoparticles, the spectral properties and their applications in analytical chemistry, the properties, functions and analytical status of the anticancer drug 6-mercaptopurine are reviewed. A new spectrophotometric method for the determination of trace antitumor drug 6-mercaptopurine was established using gold nanoparticles as a probe. After self-assembly of 6-mercaptopurine onto gold nanoparticles, the color of the solution changed from bright red to dark blue. The absorption spectrum changed obviously. Before the reaction, gold nanoparticles appeared their typical plasmon resonance absorption band at 520 nm, while 6-mercaptopurine had no absorption peak in visible region. After the reaction, the absorbance at the absorption peak of 520 nm decreased, but a strong absorption peak appeared at 680 nm, which was the absorption peak of the bound product. Moreover, the absorbance has a good linear relationship with the concentration of 6-mercaptopurine at this wavelength, and the detection limit is 17 渭 g 路L ~ (-1) 路L ~ (-1). The concentration of 6-mercaptopurine with 0.25mg/L was determined for 11 times, and RSD was 2.9. The method has been applied to the determination of 6-mercaptopurine in human urine. The method is simple, sensitive, anti-interference and satisfactory. A new method for the determination of 6-mercaptopurine by resonance Rayleigh scattering spectroscopy was developed using gold nanoparticles as probe. In B-R buffer solution with pH of 5.50, the gold nanoparticles self-assembled with 6-mercaptopurine to form a larger volume, thus the resonance Rayleigh scattering spectra of the system were greatly enhanced. In the experiment, the resonance Rayleigh scattering spectra were studied, and some factors affecting the reaction and the influence of coexisting substances were investigated. The results show that under the optimum conditions, the concentration of 6-mercaptopurine is linearly related to the resonance Rayleigh scattering intensity in the range of 0.017 ~ 0.32 mg/L. The sensitivity of the method is high, and the detection limit (3 蟽) is 8 渭 g / L. The concentration of 6-mercaptopurine with 0.12mg/L was determined for 11 times, and RSD was 5.1. The effect of coexisting substances on the determination of 6-MP was investigated, and the method was found to have good selectivity. A new method for the determination of 6-mercaptopurine by resonance Rayleigh scattering spectroscopy was established, and the method was applied to the determination of 6-mercaptopurine in human urine with good results.
【学位授予单位】：东北大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R341

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