低剂量UVB照射经AKT通路调节HaCaT细胞凋亡与增殖
发布时间：2019-01-17 13:57
【摘要】：目的：研究低强度紫外线UVB（Ultraviolet B，280-315nm）照射HaCaT细胞后，对HaCaT细胞凋亡和增殖的影响及其影响Akt信号通路的潜在机制。 方法：3mJ/cm2、5mJ/cm2和7mJ/cm2强度的UVB分别照射HaCaT细胞18，36和72小时后，收集细胞进行细胞凋亡和增殖检测；选取5mJ/cm2强度的UVB分别照射HaCaT细胞18小时后，收集细胞进行增殖检测；提取照射后HaCaT细胞的总蛋白进行免疫印迹实验，检测Akt，Bad和Bad-p136的表达情况。 结果：3mJ/cm2、5mJ/cm2和7mJ/cm2强度的UVB照射HaCaT细胞18，36和72小时后均不影响HaCaT细胞的凋亡水平。5mJ/cm2和7mJ/cm2强度的UVB照射HaCaT细胞18和36小时后不影响HaCaT细胞的增殖能力，而5mJ/cm2和7mJ/cm2强度的UVB照射72小时后可以显著促进HaCaT细胞的增殖。免疫印迹实验证实5mJ/cm2UVB照射后18小时后，，Bad的表达量基本维持正常，而AKT和Bad-p136表达水平显著升高。 结论：3mJ/cm2、5mJ/cm2和7mJ/cm2强度的UVB照射不影响细胞的凋亡水平，然而长时间5mJ/cm-2强度的UVB照射能促进HaCaT细胞的增殖，且这种作用可能是通过Akt信号通路进行调节，这可能是紫外线照射相关皮肤肿瘤发生发展的潜在危险因素。
[Abstract]:Aim: to study the effects of low-intensity ultraviolet UVB (Ultraviolet Bon 280-315 nm irradiation on apoptosis and proliferation of HaCaT cells and the potential mechanism of affecting Akt signaling pathway. Methods: HaCaT cells were irradiated with 3mJ / cm2 5mJ / cm2 and UVB with 7mJ/cm2 intensity for 1836 hours and 72 hours, respectively. Cell apoptosis and proliferation were detected. After the HaCaT cells were irradiated with UVB with 5mJ/cm2 intensity for 18 hours, the cells were collected for proliferation detection, and the total protein of HaCaT cells was extracted for Western blot assay to detect the expression of Akt,Bad and Bad-p136. Results: the apoptosis level of HaCaT cells was not affected by 3mJ / cm2 and 7mJ/cm2 intensity UVB irradiation for 1836 and 72 hours, but the proliferation ability of HaCaT cells was not affected by 5mJ/cm2 and 7mJ/cm2 UVB irradiation on HaCaT cells for 18 and 36 hours. The proliferation of HaCaT cells was significantly promoted by 5mJ/cm2 and 7mJ/cm2 intensive UVB irradiation for 72 hours. Western blot analysis showed that the expression of Bad remained normal after 18 hours of 5mJ/cm2UVB irradiation, while the expression of AKT and Bad-p136 increased significantly. Conclusion: UVB irradiation with 3mJ / cm2 and 7mJ/cm2 intensity does not affect the apoptosis level of HaCaT cells. However, UVB irradiation with 5mJ/cm-2 intensity for a long time can promote the proliferation of HaCaT cells, and this effect may be regulated by Akt signaling pathway. This may be a potential risk factor for the development of skin tumors associated with ultraviolet radiation.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R329.2
本文编号：2410125
[Abstract]:Aim: to study the effects of low-intensity ultraviolet UVB (Ultraviolet Bon 280-315 nm irradiation on apoptosis and proliferation of HaCaT cells and the potential mechanism of affecting Akt signaling pathway. Methods: HaCaT cells were irradiated with 3mJ / cm2 5mJ / cm2 and UVB with 7mJ/cm2 intensity for 1836 hours and 72 hours, respectively. Cell apoptosis and proliferation were detected. After the HaCaT cells were irradiated with UVB with 5mJ/cm2 intensity for 18 hours, the cells were collected for proliferation detection, and the total protein of HaCaT cells was extracted for Western blot assay to detect the expression of Akt,Bad and Bad-p136. Results: the apoptosis level of HaCaT cells was not affected by 3mJ / cm2 and 7mJ/cm2 intensity UVB irradiation for 1836 and 72 hours, but the proliferation ability of HaCaT cells was not affected by 5mJ/cm2 and 7mJ/cm2 UVB irradiation on HaCaT cells for 18 and 36 hours. The proliferation of HaCaT cells was significantly promoted by 5mJ/cm2 and 7mJ/cm2 intensive UVB irradiation for 72 hours. Western blot analysis showed that the expression of Bad remained normal after 18 hours of 5mJ/cm2UVB irradiation, while the expression of AKT and Bad-p136 increased significantly. Conclusion: UVB irradiation with 3mJ / cm2 and 7mJ/cm2 intensity does not affect the apoptosis level of HaCaT cells. However, UVB irradiation with 5mJ/cm-2 intensity for a long time can promote the proliferation of HaCaT cells, and this effect may be regulated by Akt signaling pathway. This may be a potential risk factor for the development of skin tumors associated with ultraviolet radiation.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R329.2
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相关期刊论文 前2条
1 王淑安;潘建玲;孙瑞;;环氧化酶-2的表达与皮肤鳞状细胞癌的关系[J];哈尔滨医药;2007年03期
2 朱东宁;简强;薛柯;刘博强;王玲;李承新;;UVB照射对PIG1细胞增殖活性的影响[J];中国美容医学;2011年06期
本文编号：2410125
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