年青人骨髓间充质干细胞来源生物活性因子对年老人骨髓间充质干细胞功能的影响
发布时间：2019-01-19 13:41
【摘要】：目的探讨年青人骨髓间充质干细胞(h MSCs)来源生物活性因子对年老人h MSCs功能的影响。方法低氧无血清培养条件下获得年青及年老h MSCs条件培养基(CM)。将年老h MSCs置于年青h MSCs来源CM中,或相反将年青h MSCs置于年老h MSCs来源CM中。通过Transwell实验、油红O染色、茜素红染色、β半乳糖苷酶染色、qRT-PCR分别检测不同来源CM中生物活性因子对h MSCs迁移能力、向脂肪细胞及成骨细胞分化能力、衰老、microRNA表达的影响。结果年青h MSCs来源CM生物活性因子可促进年老h MSCs功能再生,增强迁移能力和向脂肪细胞及成骨细胞分化能力,减少衰老。相反,将年青h MSCs置于年老h MSCs来源CM中,年青h MSCs功能受损,迁移能力下降,向脂肪细胞及成骨细胞分化能力下降,衰老增加。qRT-PCR检测发现,年青h MSCs来源CM生物活性因子可促进h MSCs中miR-10a表达,而年老h MSCs来源CM生物活性因子可抑制其表达。结论年青h MSCs来源生物活性因子可促进miR-10a表达,促进h MSCs迁移、分化,减少细胞衰老,促进年老h MSCs功能再生。
[Abstract]:Objective to investigate the effects of bioactive factors derived from (h MSCs) from bone marrow mesenchymal stem cells (BMSCs) on the function of MSCs in the elderly. Methods (CM). Was obtained from young and old MSCs conditioned medium under hypoxic serum-free culture condition. The old h MSCs was placed in the young h MSCs source CM, or the younger h MSCs was placed in the old h MSCs source CM. Transwell assay, oil red O staining, alizarin red staining, 尾 -galactosidase staining and qRT-PCR were used to detect the ability of bioactive factors in CM to migrate to h MSCs, differentiate into adipocytes and osteoblasts, and senescence. The effect of microRNA expression. Results the bioactive factor of CM derived from young h MSCs could promote the functional regeneration of MSCs, enhance the ability of migration and differentiation into adipocytes and osteoblasts, and reduce senescence. On the contrary, the young h MSCs was placed in the aged h MSCs source CM, the function of the young h MSCs was impaired, the migration ability was decreased, the differentiation ability to adipocytes and osteoblasts was decreased, and the senescence was increased. The bioactive factor of CM derived from young h MSCs could promote the expression of miR-10a in h MSCs, but the bioactive factor derived from CM from old h MSCs could inhibit its expression. Conclusion the bioactive factor derived from young h MSCs can promote the expression of miR-10a, promote the migration and differentiation of h MSCs, reduce cell senescence and promote the functional regeneration of h MSCs.
【作者单位】： 广州医科大学附属第二医院广州心血管疾病研究所;
【基金】：国家自然科学基金资助项目(编号:81401156) 广州市医药卫生科技项目(编号:20141A011085)
【分类号】：R329
[Abstract]:Objective to investigate the effects of bioactive factors derived from (h MSCs) from bone marrow mesenchymal stem cells (BMSCs) on the function of MSCs in the elderly. Methods (CM). Was obtained from young and old MSCs conditioned medium under hypoxic serum-free culture condition. The old h MSCs was placed in the young h MSCs source CM, or the younger h MSCs was placed in the old h MSCs source CM. Transwell assay, oil red O staining, alizarin red staining, 尾 -galactosidase staining and qRT-PCR were used to detect the ability of bioactive factors in CM to migrate to h MSCs, differentiate into adipocytes and osteoblasts, and senescence. The effect of microRNA expression. Results the bioactive factor of CM derived from young h MSCs could promote the functional regeneration of MSCs, enhance the ability of migration and differentiation into adipocytes and osteoblasts, and reduce senescence. On the contrary, the young h MSCs was placed in the aged h MSCs source CM, the function of the young h MSCs was impaired, the migration ability was decreased, the differentiation ability to adipocytes and osteoblasts was decreased, and the senescence was increased. The bioactive factor of CM derived from young h MSCs could promote the expression of miR-10a in h MSCs, but the bioactive factor derived from CM from old h MSCs could inhibit its expression. Conclusion the bioactive factor derived from young h MSCs can promote the expression of miR-10a, promote the migration and differentiation of h MSCs, reduce cell senescence and promote the functional regeneration of h MSCs.
【作者单位】： 广州医科大学附属第二医院广州心血管疾病研究所;
【基金】：国家自然科学基金资助项目(编号:81401156) 广州市医药卫生科技项目(编号:20141A011085)
【分类号】：R329
【参考文献】
相关期刊论文 前2条
1 黎佼;陈敏生;杨伟健;张振辉;钟峗;刘世明;;年轻骨髓间充质干细胞可通过细胞融合改善年老骨髓间充质干细胞功能[J];中国病理生理杂志;2010年05期
2 黎佼;董s，
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