Ghrelin和Obestatin对大鼠空间学习记忆能力的影响

发布时间：2019-02-14 09:59

【摘要】： 背景资料Ghrelin是1999年KOJIMA等发现的生长素促分泌素受体1a(GHS-R1a)的内源性配体。大量实验表明,Ghrelin具有很强的刺激生长激素释放、促进摄食、体重的增加等诸多作用。由于Ghrelin在机体饥饿状态下主要由胃黏膜泌酸腺A细胞分泌,因此大量实验集中于Ghrelin对摄食的启动作用和能量平衡的调节作用的研究。最近的研究表明脑室和外周给予Ghrelin具有促进正常大鼠学习记忆的能力。Obestatin与Ghrein是来源于同一个基因的肽类激素,对它的报道目前有许多争议。ZHANG等指出大鼠腹腔和脑室内注射Obestatin均可以抑制摄食;但是有研究表明Ghrelin可借助特殊的转运系统通过血-脑屏障,而Obestatin却不能通过,并且它在血液中很快地被降解;HOLST等甚至认为Obestatin并非GPR39的配体;Gourcerol发现腹腔内注射Obestatin并不影响累大鼠食量。关于Obestatin对于学习记忆的影响,CARLINI通过跳台实验和十字迷宫实验指出Obestatin有增强记忆和促焦虑的作用。目标探讨Ghrelin和Obestatin对正常大鼠空间学习记忆能力的影响。方法大鼠随机分为Ghrelin高剂量、低剂量和等体积生理盐水组;Obestatin高剂量、低剂量和等体积生理盐水组。Ghrelin组双侧海马CA3区微量注射不同剂量Ghrelin和等量生理盐水;Obestatin组侧脑室微量注射不同剂量的Obestatin和等量生理盐水。应用水迷宫方法检测大鼠的空间学习记忆能力。结果水迷宫隐匿平台实验中Ghrelin 0.3 nmol组的潜伏期与等量生理盐水组相比明显缩短,撤平台实验中Ghrelin 0.3 nmol组在原象限游泳的时间和总路程的百分比与对照组相比明显增加。Obestatin各组在隐匿平台和撤平台实验中的结果都没有统计学差异。结论海马微量注射Ghrelin可明显提高大鼠的空间学习记忆能力,该作用与可能与Ghrelin激活海马部位有关;侧脑室注射Obestatin,在水迷宫实验中未能观察到大鼠空间学习记忆能力的提高。
[Abstract]:Background data Ghrelin is the endogenous ligand of auxin receptor 1a (GHS-R1a) found in KOJIMA et al. in 1999. A large number of experiments show that Ghrelin has a strong effect on stimulating growth hormone release, promoting food consumption and increasing body weight. Since Ghrelin is mainly secreted by the gastric mucosa and the cell A of the gastric mucosa, a large number of experiments have been focused on the effect of Ghrelin on food consumption and the regulation of energy balance. Recent studies have shown that the intraventricular and peripheral administration of Ghrelin has the ability to promote learning and memory in normal rats. Obestatin and Ghrelin are a peptide-like hormone derived from the same gene, and there are many disputes about it. ZHANG et al. indicate that both intraabdominal and intraventricular injection of Obestatin in rats can inhibit food consumption; however, studies have shown that Ghrelin can be passed through the blood-brain barrier by means of a special transport system, while Obstatin can not pass, and it is rapidly degraded in the blood; HOLST or the like is not even considered to be a ligand of the GPR39; Gourcertol found that intraabdominal injection of Obestatin did not affect the amount of food that was tired in rats. With regard to the effect of Obestatin on learning and memory, CARLINI indicated that Obestatin has the effects of enhancing memory and promoting anxiety through the experimental and cross-maze experiments. Objective To explore the ability of Ghrelin and Obestatin to study the spatial learning and memory of normal rats The rats were randomly divided into high-dose, low-dose and equal-volume saline group, and the high-dose, low-dose and equal-volume saline group. Microinjection of different doses of Ghrelin and equivalent physiological saline in the CA3 region of the two-side hippocampal CA3 region of the Ghrelin group, and the small injection of different doses of Obestatin in the lateral ventricle of the Obestatin group. and the blank of the rat is detected by using the water maze method. Results The incubation period of Ghrelin 0. 3 nmol in the water maze concealed platform experiment was significantly shorter than that of the equivalent saline group, and the percentage of the time and total distance of Ghrelin 0. 3 nmol in the original quadrant was the same as that of the normal saline group. Compared with the control group, the Obestatin groups were in the test of the hidden platform and the withdrawal platform. Results There was no statistical difference in the results. Conclusion The microinjection of Ghrelin in the hippocampus can significantly improve the spatial learning and memory ability of the rats, which may be related to the activation of the hippocampal part in Ghrelin, and the injection of Obestatin in the lateral ventricle can not be seen in the water maze test.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R33

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