重庆市霍乱弧菌的分子流行病学研究
发布时间：2019-02-19 09:15
【摘要】： 本课题以霍乱弧菌特异性的霍乱肠毒素A亚单位基因(ctxA)、毒素协同调节菌毛A基因(tcpA)和全DNA序列为目标,通过聚合酶链反应(PCR)技术、限制性内切酶技术、脉冲场凝胶电泳技术和BioNumerics V4.0软件UPGMA方法进行聚类分析,观察了来源于重庆市1992年至2006年的疫情和环境检测样本的94株霍乱弧菌核酸分子特征。 着重探索霍乱暴发疫情分离的菌株与菌株之间,疫情分离的菌株与海、水产品监测分离的霍乱弧菌之间的遗传相关性,推断疫情菌株的来源、变迁及分子指纹图谱变化规律,以期为预测疫情和制定防治措施提供依据。 研究结果显示: 1、94株霍乱弧菌样本(83株感染者样本,11株环境样本)中ctxA基因阳性检出92株(97.87%),2株水产品市场监测分离菌株为阴性;tcpA基因阳性检出91株(96.81%),2株水产品市场监测分离菌株和1株来源于病人的分离株为阴性。 2、94株霍乱弧菌被分为44种脉冲场凝胶电泳图谱,聚类分析将大部分菌株分为三类(小川型,稻叶型,O139群)。 3、2002年重庆市部分区县多点暴发霍乱疫情埃尔托稻叶型1d霍乱弧菌菌株基因同源性较高;1992-2006年重庆市埃尔托小川型和埃尔托稻叶型相似系数仅为75%;从甲鱼分离的O139群霍乱弧菌与同期流行的O139群霍乱弧菌优势菌株的脉冲场凝胶电泳图谱型别一致,并且也为产毒株。 由此可见,1992-2006年重庆市霍乱弧菌流行株存在紧密相关的核酸分子特征;经过长期的霍乱流行后埃尔托霍乱弧菌主要向小川型和稻叶型分化,两型分子特征差异较大;甲鱼中分离的O139群霍乱弧菌与霍乱疫情分离菌株之间高度同源,被污染的甲鱼可能是近年来食源性霍乱暴发的主要传染来源之一,提示:海、水产品的监测应当是霍乱防控的重点。
[Abstract]:The aim of this study was to co-regulate the (tcpA) and DNA sequences of the pili A gene of Vibrio cholerae specific enterotoxin A subunit gene (ctxA), toxin, and to use polymerase chain reaction (PCR) technique and restriction endonuclease technique. The nucleic acid molecular characteristics of 94 strains of Vibrio cholerae from 1992 to 2006 in Chongqing were observed by cluster analysis with pulsed field gel electrophoresis (PGE) technique and UPGMA method of BioNumerics V4.0 software. The genetic correlation between strains isolated from cholera outbreak and strains, between strains isolated from epidemic situation and sea, and between Vibrio cholerae isolated by aquatic product monitoring was explored, and the source, changes and variation rules of molecular fingerprints of the strains were inferred. In order to predict the epidemic situation and formulate prevention and control measures to provide the basis. The results showed that: 1 92 strains (97.87%) were positive for ctxA gene in 94 strains of Vibrio cholerae (83 infected strains, 11 environmental samples), and 2 strains were negative in aquatic products market monitoring. 91 strains (96.81%) were positive for tcpA gene, 2 strains were negative for aquatic products market monitoring and 1 strain was isolated from patients. Two hundred and ninety-four strains of Vibrio cholerae were divided into 44 kinds of pulsed field gel electrophoresis patterns, and most of them were classified into three groups (Ogawa type, rice leaf type, O139 group) by cluster analysis. (3) in 2002, there was a high homology among vibrio cholerae strains of Eltorice leaf type 1d in some districts and counties of Chongqing in 2002, and the similarity coefficient of leaf type between Erto Xiaochuan type and Elto leaf type in Chongqing from 1992 to 2006 was only 75%. The dominant strains of Vibrio cholerae O139 isolated from Turtle were identical to the dominant strains of Vibrio cholerae O139 in the same period, and were also virulent strains. It can be seen that the epidemic strains of Vibrio cholerae in Chongqing from 1992 to 2006 had closely related nucleic acid molecular characteristics, and after a long period of cholera epidemic, Vibrio cholerae mainly differentiated into Ogawa type and rice leaf type, and the molecular characteristics of the two types were quite different. The isolates of Vibrio cholerae O139 isolated from turtle have high homology with the isolates of cholera epidemic situation. The contaminated turtle may be one of the main infectious sources of foodborne cholera outbreaks in recent years. Surveillance of aquatic products should be the focus of cholera prevention and control.
【学位授予单位】：第三军医大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R378
本文编号：2426355
[Abstract]:The aim of this study was to co-regulate the (tcpA) and DNA sequences of the pili A gene of Vibrio cholerae specific enterotoxin A subunit gene (ctxA), toxin, and to use polymerase chain reaction (PCR) technique and restriction endonuclease technique. The nucleic acid molecular characteristics of 94 strains of Vibrio cholerae from 1992 to 2006 in Chongqing were observed by cluster analysis with pulsed field gel electrophoresis (PGE) technique and UPGMA method of BioNumerics V4.0 software. The genetic correlation between strains isolated from cholera outbreak and strains, between strains isolated from epidemic situation and sea, and between Vibrio cholerae isolated by aquatic product monitoring was explored, and the source, changes and variation rules of molecular fingerprints of the strains were inferred. In order to predict the epidemic situation and formulate prevention and control measures to provide the basis. The results showed that: 1 92 strains (97.87%) were positive for ctxA gene in 94 strains of Vibrio cholerae (83 infected strains, 11 environmental samples), and 2 strains were negative in aquatic products market monitoring. 91 strains (96.81%) were positive for tcpA gene, 2 strains were negative for aquatic products market monitoring and 1 strain was isolated from patients. Two hundred and ninety-four strains of Vibrio cholerae were divided into 44 kinds of pulsed field gel electrophoresis patterns, and most of them were classified into three groups (Ogawa type, rice leaf type, O139 group) by cluster analysis. (3) in 2002, there was a high homology among vibrio cholerae strains of Eltorice leaf type 1d in some districts and counties of Chongqing in 2002, and the similarity coefficient of leaf type between Erto Xiaochuan type and Elto leaf type in Chongqing from 1992 to 2006 was only 75%. The dominant strains of Vibrio cholerae O139 isolated from Turtle were identical to the dominant strains of Vibrio cholerae O139 in the same period, and were also virulent strains. It can be seen that the epidemic strains of Vibrio cholerae in Chongqing from 1992 to 2006 had closely related nucleic acid molecular characteristics, and after a long period of cholera epidemic, Vibrio cholerae mainly differentiated into Ogawa type and rice leaf type, and the molecular characteristics of the two types were quite different. The isolates of Vibrio cholerae O139 isolated from turtle have high homology with the isolates of cholera epidemic situation. The contaminated turtle may be one of the main infectious sources of foodborne cholera outbreaks in recent years. Surveillance of aquatic products should be the focus of cholera prevention and control.
【学位授予单位】：第三军医大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R378
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