重编程细胞的命运

发布时间：2019-02-22 12:04

【摘要】：背景:体细胞重新编程技术也称细胞重组技术,使已经完成分化的体细胞回到原始的全能性或多能性状态,并可以重新分化成与原来不一样的细胞。通过重编程技术可以获得患者特异性诱导多能干细胞和疾病特异性诱导多能干细胞,显著减少了免疫排斥反应。目的:探讨关于直接重编程到特定系的方法,总结参与重编程的分子机制。方法:以"重编程"为中文检索词,"reprogramming"为英文检索词,应用计算机检索维普(VIP)期刊全文数据库、万方全文数据库、中国知网全文数据库、PubM ed数据库、Springer数据库1958年1月至2015年4月有关细胞重编程技术的文献,排除与研究目的无关及重复性研究,保留40篇文献进一步分析。结果与结论:当前重编程的步骤效率很低,在特定群只有相对少量的细胞能进行重编程,重编程的完整性和程度也有待证实。直接重编程成体、定系的细胞从一种细胞到另一种细胞一直是发育生物学很难达到的目标。最近的研究证明分化的细胞强制表达特异转录因子能促进细胞分化。这些发现使再生医学领域有了重大进展,可以提供替代细胞治疗各种再生紊乱。目前,基本的分子机制需要进一步阐明,在直接重编程被应用于临床之前还有许多问题需要解决。
[Abstract]:Background: somatic cell reprogramming, also known as cell recombination, enables the differentiated somatic cells to return to the original totipotency or pluripotent state, and can be redifferentiated into different cells. The specific induced pluripotent stem cells and disease-specific pluripotent stem cells can be obtained by reprogramming technique, and the immune rejection can be significantly reduced. Aim: to explore the methods of direct reprogramming to specific systems and summarize the molecular mechanisms involved in reprogramming. Methods: using "reprogramming" as the Chinese key word and "reprogramming" as the English key word, the full text database of Weipu (VIP), Wanfang full-text database and, PubM ed database were searched by computer. From January 1958 to April 2015, Springer database related to cell reprogramming technology, excluding the research objectives and repeatability, and retaining 40 articles for further analysis. Results & conclusion: at present, the efficiency of reprogramming is very low, only a relatively small number of cells can be reprogrammed in a specific population, and the integrity and degree of reprogramming need to be proved. Direct reprogramming of adult cells from one cell to another has been a difficult goal in developmental biology. Recent studies have shown that forced expression of specific transcription factors in differentiated cells promotes cell differentiation. These findings have led to significant advances in regenerative medicine, providing alternative cells for the treatment of various regenerative disorders. At present, the basic molecular mechanism needs to be further clarified, and there are still many problems to be solved before direct reprogramming is applied in clinical practice.
【作者单位】：解放军成都军区昆明总医院细胞生物治疗中心干细胞与免疫细胞生物医药技术国家地方联合工程实验室云南省细胞治疗技术转化医学重点实验室;
【基金】：国家支撑计划(2014BAI01B01) 云南省科技计划项目(2013CA005)资助课题~~
【分类号】：R329.2

【共引文献】