链脲佐菌素诱导急性非糖尿病小鼠胰岛β细胞凋亡模型的建立

发布时间：2019-03-01 09:22

【摘要】：目的以非致糖尿病的小剂量链脲佐菌素(STZ)诱导BABL/C小鼠胰岛β细胞发生凋亡,建立小鼠急性损伤胰岛细胞凋亡的模型,明确其诱导胰岛β细胞凋亡的发生规律,为胰岛β细胞凋亡相关研究提供在体模型。方法选取BABL/C小鼠,分别予生理盐水(对照组)及60、80、100 mg/kg小剂量STZ一次性腹腔注射(实验组),观察时点分别为干预后6、12和24 h,动态观察指标为血糖、胰腺HE染色观察病理改变以及末端脱氧核苷酸转移酶介导的d UTP缺口标记技术(TUNEL)检测胰岛β细胞凋亡。结果在所有测试时点血糖均在6~11mmol/L范围内波动,未达到糖尿病模型范畴,TUNEL凋亡检测发现:与对照组相比,实验组各时点的胰岛β细胞凋亡率均明显升高(P0.05);各实验组均在12 h时点凋亡率达到最高峰,并且80 mg/kg组与60 mg/kg、100 mg/kg组相比明显升高(P0.05)。结论非致糖尿病的小剂量STZ可成功构建急性非糖尿病小鼠胰岛β细胞凋亡模型,凋亡高峰期在注射后12 h时点。
[Abstract]:Objective to induce apoptosis of islet 尾 cells in BABL/C mice by low dose streptozotocin (STZ) without diabetes, and to establish an acute injury model of apoptosis of islet cells in mice, and to clarify the regularity of apoptosis of 尾 cells induced by streptozotocin in mice. It provides an in vivo model for the study of islet 尾 cell apoptosis. Methods BABL/C mice were injected intraperitoneally with saline (control group) and low dose STZ (60,80100 mg/kg) respectively (experimental group). The observation time points were 6, 12 and 24 hours after intervention, and the dynamic indexes were blood glucose. The pathological changes of pancreatic islet were observed by HE staining, and the apoptosis of pancreatic 尾 cells was detected by (TUNEL), which was mediated by terminal deoxynucleotidyl transferase-mediated d-UTP Nick labeling technique. Results Blood glucose fluctuated in the range of 6~11mmol/L at all time points, and did not reach the category of diabetic model. TUNEL apoptosis detection showed that: compared with the control group, the apoptosis rate of islet 尾 cells in the experimental group increased significantly at each time point (P0.05); The apoptosis rate reached the peak at 12 h in each experimental group, and it was significantly higher in 80 mg/kg group than that in 60 mg/kg,100 mg/kg group (P0.05). Conclusion small dose of STZ without diabetes can successfully establish 尾-cell apoptosis model in acute non-diabetic mice, and the peak of apoptosis is at 12 h after injection.
【作者单位】：广西医科大学第一附属医院内分泌科;广西医科大学病理生理教研室;广西南宁市第一人民医院内分泌科;
【基金】：广西自然科学基金资助项目(编号:2013GXNSFAA019202)
【分类号】：R-332;R587.1

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