针刀干预椎动脉型颈椎病模型兔的实验研究

发布时间：2019-03-08 13:33

【摘要】：目的:观察针刀干预椎动脉型颈椎病(cervical spondylosis of vertebral artery type,CSA)模型兔肌肉组织光镜下的形态学变化和血清一氧化氮(nitric oxide,NO)、血浆内皮素(endothelin,ET)含量的影响,探讨针刀干预椎动脉型颈椎病的部分机制。方法:选用雌性新西兰家兔25只,取5只作为空白对照组,剩余造模成功的15只随机分为模型组、电针组、针刀组,各5只。空白组、模型组不干预;电针组穴取"百会""风池""颈1~3夹脊",针刺得气后,连接华佗牌SDZ-Ⅱ型电子针灸仪,每次20 min,每天1次,10天为一疗程,疗程间休息1天,共2个疗程;针刀组在C_3~C_5棘突旁开1.5~2 cm处予针刀治疗,7天1次,共3次。观察各组家兔颈部肌肉组织形态学变化,应用硝酸还原酶法检测各组血清NO的含量,采用放射免疫法检测各组血浆ET的含量。结果:与电针组相比,光镜下观察针刀组干预后针切部位肌细胞轻度坏死,间质间未见明显的炎性细胞浸润,针切断裂部位趋向于正常骨骼肌及筋膜结构结缔组织修复。模型组血清NO含量低于空白对照组(P0.01),血浆ET含量高于空白对照组(P0.01);电针组与针刀组血清NO含量高于模型组(均P0.05),血浆ET含量低于模型组(均P0.05);针刀组家兔血浆ET含量明显低于电针组(P0.05)。结论:针刀干预通过促进炎性细胞消散,改善肌肉组织修复,提升血清NO含量,降低血浆ET含量,使椎动脉维持在一种正常的生理舒张状态,保证了脑组织的正常灌注,可能是针刀干预治疗CSA的部分作用机制。
[Abstract]:Objective: to observe the morphological changes of muscle tissue and the level of serum nitric oxide (nitric oxide,NO) and plasma endothelin (endothelin,ET) in rabbits with cervical spondylosis of vertebral artery type (cervical spondylosis of vertebral artery type,CSA) treated by acupuncture knife. To explore the mechanism of acupuncture-knife intervention in cervical spondylosis of vertebral artery type. Methods: fifteen female New Zealand rabbits were randomly divided into three groups: model group (n = 15), electro-acupuncture group (n = 5), acupuncture group (n = 5) and blank control group (n = 5). Blank group, model group, non-intervention; In the electro-acupuncture group, "Baihui", "Fengchi" and "neck 1-3 clip ridge" were taken. After acupuncture, Huatuo brand SDZ- 鈪，

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