2种高尿酸血症及相关并发症动物模型的建立与比较研究

发布时间：2019-03-18 12:02

【摘要】：目的采用饮食诱导法分别建立鹌鹑及大鼠高尿酸血症模型,观察模型的发病过程,比较2种模型的病理特征。方法 20只雄性迪法克鹌鹑随机分为正常组鹌鹑、模型组鹌鹑,正常组给予普通鹌鹑饲料,模型组鹌鹑给予高嘌呤饲料;20只雄性SD大鼠随机分为正常组大鼠、模型组大鼠,正常组大鼠给予普通大鼠饲料,模型组大鼠给予高果糖饲料:检测各组动物的血清尿酸、三酰甘油、血糖水平及相关代谢酶活性。结果各组动物体质量呈自然增长趋势。模型组鹌鹑血清尿酸水平显著升高,造模后期血清三酰甘油水平显著升高(P0.05),血清黄嘌呤氧化酶活性显著升高(P0.05)。模型组大鼠血清三酰甘油水平显著升高,造模后期血清尿酸水平显著升高(P0.05)。结论高嘌呤及高果糖饲料可成功建立高尿酸血症并高甘油三酯血症动物模型。鹌鹑及大鼠高尿酸并高甘油三酯血症模型的病理表现及相关代谢酶变化不尽相同,可根据研究需要选择动物模型。
[Abstract]:Aim to establish the hyperuricemia model of quail and rat by diet induction method, observe the pathogenesis of the model and compare the pathological characteristics of the two models. Methods Twenty male diflac quails were randomly divided into normal group, model group, normal group and model group. The quails in the model group were fed with high purine diet. The quails in the model group were fed with high purine diet. Twenty male SD rats were randomly divided into normal group, model group, normal group and high fructose diet. Blood glucose level and related metabolic enzyme activity. Results the mass of animals and objects in each group showed a natural increasing trend. In the model group, the serum uric acid level was significantly increased, the serum triacylglycerol level was significantly increased (P0.05), and the serum xanthine oxidase activity was significantly increased in the model group (P0.05). The levels of serum triacylglycerol and uric acid in model group were significantly higher than those in model group (P0.05). Conclusion the animal model of hyperuricemia and hypertriglyceridemia can be successfully established with high purine and high fructose diet. The pathological manifestations and metabolic enzymes changes of quail and rat models of hyperuricemia and hypertriglyceridemia are different. Animal models can be selected according to the needs of the study.
【作者单位】：北京中医药大学中药学院;
【基金】：国家自然科学基金(81274153) 教育部高等学校博士学科点专项科研基金(20130013120001,20120013130002) 北京中医药大学“重点学科”开放课题(2013-ZDXKKF-19)
【分类号】：R589.7;R-332

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