慢性饮茶型氟铝联合中毒动物模型建立及其对机体的相关影响
发布时间：2019-03-21 12:21
【摘要】：目的依牧民饮茶习惯建立大鼠慢性砖茶型氟铝联合中毒和低氟低铝砖茶模型,观察分析砖茶中氟铝对大鼠体重、学习记忆、神经系统、血液系统、骨骼系统的影响及各组织的氟铝含量,探讨砖茶型氟铝联合中毒的病理生理机制。为根治砖茶型氟铝联合中毒提供理论依据。方法离乳4周的雄性Wistar大鼠24只、雌性36只,随机分为6组。空白对照组(饮自来水)、单铝组(饮水含铝159mg/L)、单氟组(饮水含氟100mg/L)、氟铝联合组(饮水含铝159mg/L、含氟100mg/L)、砖茶组(饮砖茶水)、低氟普洱茶组(饮低氟普洱茶水)。各组皆以配制的饮品为饮水唯一来源,自由饮食,实验中每三个月测大鼠体重；实验最后两个月对其学习记忆行为评价；实验结束(一年)后处死,测其脑组织中生长抑素含量；观察脑、肝、脾、肾的微观结构；并测血常规及血清、肾、肝、骨、牙、脑中的氟、铝含量。实验所得数据以均数±标准差(X±S)表示,用SPSS13.0软件进行统计学分析处理,检验水准取α=0.05。结果铝可增加大鼠体重(p0.05)；氟铝联合比单铝的作用慢但持久且严重(p0.05)；低氟普洱茶能使一年龄大鼠体重下降(p0.05)。铝引起学习记忆功能障碍(p0.05)；氟铝联合比单铝的作用严重(p0.01)；砖茶亦引起学习记忆功能障碍(p0.05)。氟、铝都引起大鼠海马SS数量下降(p0.05)；氟铝联合比单铝作用严重(p0.01)；砖茶比单氟作用严重(p0.01)。高浓度氟能提高大鼠的MCV、PLT(p0.05);低氟普洱茶能降低大鼠HCT、MCV、MCHC(P0.05)。高浓度铝可促进氟吸收并在血清、骨、牙、脑、肝中蓄积；高浓度铝可蓄积在血清、骨、牙、脑中(p0.05),高浓度氟可抑制铝吸收。砖茶引起骨、牙、肝氟含量增加(p0.05)及牙、肝铝含量增加(p0.05)；低氟普洱茶引起肾、肝铝含量增加(p0.05)。氟铝联合和单铝组大鼠脑组织出现脑水肿；氟铝联合和单氟组肾出现蛋白尿；氟铝联合和砖茶组肝出现小面积的点状坏死灶。结论1、成功复制了砖茶型氟铝联合中毒大鼠动物模型。2、氟元素针对的靶器官是骨和牙,并能引起相应的损伤。铝元素针对的靶器官是骨、牙及脑,能引起生长发育障碍及学习记忆功能障碍。3、氟铝同时存在时,高浓度铝可促进氟吸收,高浓度氟可抑制铝吸收。4、选用低氟普洱茶代替普通砖茶,可在不影响砖茶口味和色泽的前提下,预防砖茶型氟铝联合中毒的发牛
[Abstract]:Objective to establish chronic brick tea poisoning and low fluoride and low aluminum brick tea models in rats according to herdsman's tea drinking habits, and to observe and analyze the effects of fluoride and aluminum in brick tea on rats' weight, learning and memory, nervous system and blood system. The effects of skeletal system and the content of fluoride and aluminum in tissues were studied to explore the pathophysiological mechanism of brick-tea fluoro-aluminum poisoning. It provides a theoretical basis for the radical cure of combined fluoro-aluminum poisoning in brick tea. Methods 24 male Wistar rats and 36 female rats were randomly divided into 6 groups. Blank control group (drinking tap water), single aluminum group (drinking water containing aluminum 159mg/L), single fluoride group (drinking water containing fluoride 100mg/L), fluoride and aluminum combination group (drinking water containing aluminum 159 mg / L, fluoride containing 100mg/L), brick tea group (drinking brick tea water), Low fluoride Pu'er tea group (drinking low fluorine Pu'er tea water). The rats' weight was measured every three months, and the learning and memory behaviors of rats were evaluated at the last two months of the experiment, and the rats in each group were treated with the drink as the only source of drinking water, and the rats were given a free diet at the last two months. The contents of somatostatin in brain tissue, microstructure of brain, liver, spleen and kidney, blood routine test, serum, kidney, liver, bone, tooth and brain were measured. The experimental data were expressed as mean 卤standard deviation (X 卤S) and analyzed by SPSS13.0 software. The level was 伪 = 0.05. Results Aluminum could increase the body weight of rats (p0.05), the effect of fluoride-aluminum combination was slower but longer and more serious than that of single aluminum (p0.05), and low-fluorine Pu-erh tea could decrease the weight of one-year-old rats (p0.05). Aluminum induced learning and memory dysfunction (p0.05), fluoride combined with aluminum had more effect than aluminum (p0.01), brick tea also caused learning and memory dysfunction (p0.05). Fluoride and aluminum could decrease the number of SS in hippocampus of rats (p0.05), the effect of fluoride combined with aluminum was more serious than that of single aluminum (p0.01), and the effect of brick tea was more serious than that of monofluoride (p0.01). High concentration of fluoride could increase the MCV,PLT of rats (p0.05), and low fluoride Pu'er tea could decrease the HCT,MCV,MCHC of rats (P0.05). High concentration of aluminum can promote fluoride absorption and accumulate in serum, bone, tooth, brain and liver; high concentration of aluminum can accumulate in serum, bone, tooth and brain (p0.05), and high concentration of fluoride can inhibit aluminum absorption. Brick tea increased fluoride content in bone, tooth and liver (p0.05) and aluminum content in tooth and liver (p0.05), and low fluoride Pu'er tea increased aluminum content in kidney and liver (p0.05). There were brain edema in brain tissue, proteinuria in kidney and small area of punctate necrosis of liver in fluoride-aluminum group and brick-tea group in the combination of fluoride-aluminum group and single-aluminum group, and the brain edema in brain tissue, proteinuria in the kidney of fluoride-aluminum combination group and single fluoride group. Conclusion 1. The animal model of brick tea-type fluoride-aluminum poisoning rats was successfully reproduced. 2. The target organs targeted by fluoride elements were bone and tooth and could cause corresponding damage. 2. The target organs targeted by aluminum elements are bone, tooth and brain, which can cause growth disorder and learning and memory dysfunction. 3. When fluoride and aluminum exist simultaneously, high concentration of aluminum can promote the absorption of fluoride, and high concentration of fluoride can inhibit the absorption of aluminum. Using low fluorine Pu'er tea instead of ordinary brick tea can prevent the poisoning of brick tea with fluorine and aluminum on the premise of not affecting the taste and color of brick tea.
【学位授予单位】：内蒙古医学院
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R599;R-332
本文编号：2444928
[Abstract]:Objective to establish chronic brick tea poisoning and low fluoride and low aluminum brick tea models in rats according to herdsman's tea drinking habits, and to observe and analyze the effects of fluoride and aluminum in brick tea on rats' weight, learning and memory, nervous system and blood system. The effects of skeletal system and the content of fluoride and aluminum in tissues were studied to explore the pathophysiological mechanism of brick-tea fluoro-aluminum poisoning. It provides a theoretical basis for the radical cure of combined fluoro-aluminum poisoning in brick tea. Methods 24 male Wistar rats and 36 female rats were randomly divided into 6 groups. Blank control group (drinking tap water), single aluminum group (drinking water containing aluminum 159mg/L), single fluoride group (drinking water containing fluoride 100mg/L), fluoride and aluminum combination group (drinking water containing aluminum 159 mg / L, fluoride containing 100mg/L), brick tea group (drinking brick tea water), Low fluoride Pu'er tea group (drinking low fluorine Pu'er tea water). The rats' weight was measured every three months, and the learning and memory behaviors of rats were evaluated at the last two months of the experiment, and the rats in each group were treated with the drink as the only source of drinking water, and the rats were given a free diet at the last two months. The contents of somatostatin in brain tissue, microstructure of brain, liver, spleen and kidney, blood routine test, serum, kidney, liver, bone, tooth and brain were measured. The experimental data were expressed as mean 卤standard deviation (X 卤S) and analyzed by SPSS13.0 software. The level was 伪 = 0.05. Results Aluminum could increase the body weight of rats (p0.05), the effect of fluoride-aluminum combination was slower but longer and more serious than that of single aluminum (p0.05), and low-fluorine Pu-erh tea could decrease the weight of one-year-old rats (p0.05). Aluminum induced learning and memory dysfunction (p0.05), fluoride combined with aluminum had more effect than aluminum (p0.01), brick tea also caused learning and memory dysfunction (p0.05). Fluoride and aluminum could decrease the number of SS in hippocampus of rats (p0.05), the effect of fluoride combined with aluminum was more serious than that of single aluminum (p0.01), and the effect of brick tea was more serious than that of monofluoride (p0.01). High concentration of fluoride could increase the MCV,PLT of rats (p0.05), and low fluoride Pu'er tea could decrease the HCT,MCV,MCHC of rats (P0.05). High concentration of aluminum can promote fluoride absorption and accumulate in serum, bone, tooth, brain and liver; high concentration of aluminum can accumulate in serum, bone, tooth and brain (p0.05), and high concentration of fluoride can inhibit aluminum absorption. Brick tea increased fluoride content in bone, tooth and liver (p0.05) and aluminum content in tooth and liver (p0.05), and low fluoride Pu'er tea increased aluminum content in kidney and liver (p0.05). There were brain edema in brain tissue, proteinuria in kidney and small area of punctate necrosis of liver in fluoride-aluminum group and brick-tea group in the combination of fluoride-aluminum group and single-aluminum group, and the brain edema in brain tissue, proteinuria in the kidney of fluoride-aluminum combination group and single fluoride group. Conclusion 1. The animal model of brick tea-type fluoride-aluminum poisoning rats was successfully reproduced. 2. The target organs targeted by fluoride elements were bone and tooth and could cause corresponding damage. 2. The target organs targeted by aluminum elements are bone, tooth and brain, which can cause growth disorder and learning and memory dysfunction. 3. When fluoride and aluminum exist simultaneously, high concentration of aluminum can promote the absorption of fluoride, and high concentration of fluoride can inhibit the absorption of aluminum. Using low fluorine Pu'er tea instead of ordinary brick tea can prevent the poisoning of brick tea with fluorine and aluminum on the premise of not affecting the taste and color of brick tea.
【学位授予单位】：内蒙古医学院
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R599;R-332
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