维生素C增强衰老个体来源的骨髓间充质干细胞的增殖能力

发布时间：2019-03-29 13:35

【摘要】：目的观察维生素C(Vc)能否通过升高端粒酶活性促进衰老个体来源骨髓间充质干细胞(BMMSCs)的增殖能力。方法以骨特异性早衰小鼠(SAMP6)为实验组,不发生早衰的R品系小鼠(SAMR1)为对照组,采用显微CT扫描技术验证SAMP6小鼠的骨衰老表型。分离培养两组小鼠的BMMSCs,其中SAMP6小鼠来源的BMMSCs分别应用不同浓度的Vc进行处理。采用MTT法检测BMMSCs的增殖能力,绘制生长曲线,使用端粒酶试剂盒检测端粒酶活性,并用PCR和Western blot方法检测端粒酶逆转录酶(TERT)的表达水平。结果 SAMP6小鼠具有骨衰老表型,SAMP6小鼠来源的BMMSCs的增殖能力和端粒酶活性均低于SAMR1小鼠来源的BMMSCs,差异均有统计学意义(P0.05)。加入Vc后,在一定浓度范围内SAMP6小鼠来源的BMMSCs增殖能力随Vc浓度的升高而显著改善(P0.05),同时其端粒酶活性和TERT表达水平有所提高(P0.05),与增殖能力具有相关性。其中,Vc发挥促进作用的最适浓度为100μg/m L,在1000μg/m L时有抑制作用。结论维生素C能够增强衰老个体来源BMMSCs的增殖能力,且这种作用可能是通过升高端粒酶活性实现的。
[Abstract]:Objective to investigate whether vitamin C (Vc) can promote the proliferation of bone marrow mesenchymal stem cells (BMMSCs) from aging individuals by increasing telomerase activity. Methods using bone-specific premature aging mice (SAMP6) as experimental group and R strain mice (SAMR1) without premature senescence as control group, bone aging phenotype of SAMP6 mice was verified by microCT scanning technique. The BMMSCs, of two groups of mice were isolated and cultured. The BMMSCs derived from SAMP6 mice were treated with different concentrations of Vc. The proliferation ability of BMMSCs was detected by MTT method, the growth curve was drawn, telomerase activity was detected by telomerase assay kit, and the expression level of telomerase reverse transcriptase (TERT) was detected by PCR and Western blot methods. Results SAMP6 mice had bone senescence phenotype. The proliferation ability and telomerase activity of BMMSCs derived from SAMP6 mice were significantly lower than those of BMMSCs, from SAMR1 mice (P0.05). After adding Vc, the proliferation ability of BMMSCs derived from SAMP6 mice was significantly improved with the increase of Vc concentration (P0.05), and the telomerase activity and TERT expression level were increased (P0.05), which was correlated with the proliferation ability. The optimum concentration of Vc was 100 渭 g / mL and inhibited at 1000 渭 g / mL. Conclusion Vitamin C can enhance the proliferation of BMMSCs in senescent individuals, and this effect may be achieved by increasing telomerase activity.
【作者单位】：第四军医大学组织工程研发中心;第四军医大学军事口腔医学国家重点实验室;
【基金】：国家自然科学基金(31301062)~~
【分类号】：R329

【相似文献】