围孕期应用益生菌对子代大鼠肠道免疫功能的影响研究
发布时间：2019-04-02 05:55
【摘要】： 目的 妊娠期合理的营养摄入能增加子代健康的机会,孕期营养对子代的发育和远期影响越来越成为人们关注的热门话题。本研究拟通过在围孕期给母鼠服用益生菌制剂金双歧,来进一步观察围孕期应用益生菌是否对子代大鼠肠道免疫功能成熟有促进作用。 方法 健康雄性大鼠6只,健康雌性大鼠30只随机分组(按雌:雄=5:1)合笼交配。分别于交配前7天(5只)、妊娠早期(第1天始,5只),妊娠中期(第8天始,5只),妊娠晚期(第15天,5只),分娩母乳期(5只)应用益生菌制剂金双歧(双歧杆菌、乳杆菌、嗜热链球菌三联活菌片)连续灌胃给药;对照组(不用益生菌,普通喂养5只)。在分娩后第1,2,3,7,14天,采取逼迫法取子代大鼠新鲜粪便,(每只母鼠随机抽取5只子鼠)接种到改良MRS培养基上,进行厌氧培养,观察结果,记录菌落数。同时在21天采用ELISA方法测定子代大鼠(每组随机抽取14只子鼠)血清中IL-4和IFN—r水平,与对照组进行比较。 结果 1.在分娩后48小时内试验组和对照组子鼠肠道菌群中均未见双歧杆菌定植,但72小时内均发现有双歧杆菌定植。 2.怀孕前或怀孕早期服用益生菌的母鼠所分娩的子鼠血清中IL-4和IFN—r浓度高于对照组子鼠(P＜0.05),仅母乳期应用益生菌几乎不影响子代IL—4和IFN—r水平(P＞0.05)。 结论 1.孕前、妊娠期和母乳期应用益生菌其子代肠道菌群的提前定植作用不明显。 2.围孕期应用益生菌可升高子代血清IL-4和IFN—r含量,随用药时间的延长总体上血清中IL-4和IFN—r浓度有升高趋势,但幅度不同,用药时间短的和未用药的血清中IL-4和IFN—r浓度差别不是很大。 3.孕妇在孕前和妊娠期服用益生菌制剂对新生儿生后肠道免疫功能成熟有一定的促进作用。
[Abstract]:Objective reasonable nutrition intake during pregnancy can increase the chances of offspring health, and the influence of nutrition during pregnancy on the development and long-term development of offspring has become a hot topic. The aim of this study was to observe whether probiotics could promote the maturation of intestinal immune function in pregnant rats by taking the bifidus of probiotics preparation during the period of gestation in order to further observe the effect of probiotics on the maturation of intestinal immune function in the offspring rats during peri-pregnancy. Methods six healthy male rats and 30 healthy female rats were randomly divided into two groups (male = 5:1). At 7 days before mating (5), early pregnancy (1 day, 5), middle pregnancy (8 days, 5), third trimester (15 days, 5), The probiotics preparation gold bifidobacterium (Bifidobacterium, Lactobacillus, Streptococcus thermophilus triple viable bacterial tablets) was administered continuously during the milk delivery period (n = 5). Control group (not probiotics, ordinary feeding 5). On the 1st, 2nd, 3rd, 7th, 14th day after delivery, the fresh feces of the offspring rats were taken by forced method and inoculated into the modified MRS medium at random. The results were observed and the number of colonies was recorded. At the same time, the levels of IL-4 and IFN-r in the serum of 14 offspring rats were measured by ELISA method on the 21st day and compared with those of the control group. Outcome 1. Bifidobacterium colonization was not found in the intestinal flora of the test group and control group within 48 hours after delivery, but bifidobacterium colonization was found within 72 hours. 2. The serum levels of IL-4 and IFN-r in prepregnant and early pregnant mother mice were higher than those in control group (P < 0.05). Probiotics only had no effect on the levels of IL-4 and IFN-r in their offspring (P > 0.05). Conclusion 1. Before pregnancy, preimplantation of probiotics during pregnancy and breast-feeding had no significant effect on the early colonization of the intestinal flora of their offspring. 2. Probiotics could increase the levels of serum IL-4 and IFN-r in offspring during perinatal period. The concentrations of IL-4 and IFN-r in serum tended to increase with the prolongation of the treatment time, but the range was different. The concentrations of IL-4 and IFN-r in the serum of patients with and without medication were not significantly different. 3. The use of probiotics in pregnant women before and during pregnancy can promote the maturation of intestinal immune function in newborn infants.
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R371;R715
本文编号：2452305
[Abstract]:Objective reasonable nutrition intake during pregnancy can increase the chances of offspring health, and the influence of nutrition during pregnancy on the development and long-term development of offspring has become a hot topic. The aim of this study was to observe whether probiotics could promote the maturation of intestinal immune function in pregnant rats by taking the bifidus of probiotics preparation during the period of gestation in order to further observe the effect of probiotics on the maturation of intestinal immune function in the offspring rats during peri-pregnancy. Methods six healthy male rats and 30 healthy female rats were randomly divided into two groups (male = 5:1). At 7 days before mating (5), early pregnancy (1 day, 5), middle pregnancy (8 days, 5), third trimester (15 days, 5), The probiotics preparation gold bifidobacterium (Bifidobacterium, Lactobacillus, Streptococcus thermophilus triple viable bacterial tablets) was administered continuously during the milk delivery period (n = 5). Control group (not probiotics, ordinary feeding 5). On the 1st, 2nd, 3rd, 7th, 14th day after delivery, the fresh feces of the offspring rats were taken by forced method and inoculated into the modified MRS medium at random. The results were observed and the number of colonies was recorded. At the same time, the levels of IL-4 and IFN-r in the serum of 14 offspring rats were measured by ELISA method on the 21st day and compared with those of the control group. Outcome 1. Bifidobacterium colonization was not found in the intestinal flora of the test group and control group within 48 hours after delivery, but bifidobacterium colonization was found within 72 hours. 2. The serum levels of IL-4 and IFN-r in prepregnant and early pregnant mother mice were higher than those in control group (P < 0.05). Probiotics only had no effect on the levels of IL-4 and IFN-r in their offspring (P > 0.05). Conclusion 1. Before pregnancy, preimplantation of probiotics during pregnancy and breast-feeding had no significant effect on the early colonization of the intestinal flora of their offspring. 2. Probiotics could increase the levels of serum IL-4 and IFN-r in offspring during perinatal period. The concentrations of IL-4 and IFN-r in serum tended to increase with the prolongation of the treatment time, but the range was different. The concentrations of IL-4 and IFN-r in the serum of patients with and without medication were not significantly different. 3. The use of probiotics in pregnant women before and during pregnancy can promote the maturation of intestinal immune function in newborn infants.
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R371;R715
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